Comparision of iron-regulated outer membrane proteins (IrOMP) and iron-sufficient outer membrane proteins (IsOMP) of Pasteurella multocida strains of porcine origin

Abstract

An investigation was carried out to compare the effect of growth of P. multocida type A of pig origin in iron restricted and in iron sufficient media on the basis of their outer membrane protein extract. Pasteurella multocida serotype A was cultured in two ways. In one process only BHI medium was used and on the other process same medium was used which was supplemented with 2, 2'-Dipyridyl (ironrestricted medium). Both these cultures were used to obtain Outer Membrane Proteins (OMPs) by extraction of bacterial cells with 1% Sarcosyl. Separation of the OMPs in SDS-PAGE showed that OMPs were mixture of protein fraction with molecular weight ranging from 110 to 22.6 kDa in case of ironrestricted OMP and 47.3 to 29.9 kDa in case of iron sufficient OMP. The OMP with molecular masses 29.9 kDa grew in both iron-restricted as well as in iron sufficient medium.

Characterization of Pasteurella multocida and Riemerella anatipestifer of Ducks in Assam, India

“…The genomic DNA (gDNA) was extracted from the respective isolates by the snap chill method. The P. multocida-specific PCR (PM PCR) was conducted as described by Deka et al (2017). Similarly, the amplification of the R. anatipestifer-specific gene (RNAse Z gene) was done as reported by (Hazarika et al 2020).…”

“…The kmt1-positive isolates were subjected to molecular screening of capsular-specific gene(s) for capsular typing. The simplex capsular specific PCR (cap-PCR) was carried out, following the previously described protocol (Deka et al 2017) with capsular specific primers for the hyaD-hyaC (type A), bcbD (type B) and dcbF (type D) gene. The reaction was performed with previously mentioned cycling conditions, followed by electrophoresis in a 1.5% agarose gel at 80 volts for 1 hr.…”

“…The only isolate of R. anatipestifer recovered from a diseased duck Mbuthia et al (2008) reported the isolation of P. multocida from 25.9% of apparently healthy ducks. Based on the observation of the kmt1 as a marker gene, Deka et al (2017) opined that PM-PCR is a rapid, robust and highly specific confirmatory method for P. multocida, irrespective of serotypes. The capsular type A, followed by D and F have been established as the most prevalent capsular type of P. multocida in Indian duck.…”

“…The available data on the prevalence of virulence-associated gene(s) in P. multocida of avian origin reflected the consistent distribution of ompH and ompA in capsular types A and D of P. multocida. However, there may be extensive molecular mass heterogeneity in the OmpA and OmpH proteins (Deka et al 2017). Among the adhesion-associated genes, the fimA was identified in association with the pathogenic strains of P. multocida (Ewers et al 2006).…”