Comparing two metabolic profiling approaches (liquid chromatography and gas chromatography coupled to mass spectrometry) for extra-virgin olive oil phenolic compounds analysis: A botanical classification perspective

Bajoub, Aadil; Pacchiarotta, Tiziana; Hurtado‐Fernández, Elena; Olmo-García, Lucía; Garcı́a-Villalba, Rocío; Fernández‐Gutiérrez, Alberto; Mayboroda, Oleg A.

doi:10.1016/j.chroma.2015.10.059

Cited by 73 publications

(35 citation statements)

References 35 publications

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“…The use of separative techniques such as liquid (LC) or gas (GC) chromatography prior to MS detection is commonplace when analyzing complex plant derived samples [ 30 ]. Both LC/GC-MS based metabolic profiling approaches (primarily focused on the polar phenols fraction) have been used to study olive plant organs (leaves, stems, wood, roots) [ 31 , 32 , 33 ], olive fruits [ 34 , 35 ], and VOO [ 36 , 37 ]. Different coupling interfaces can be used depending on the physicochemical properties of the analytes under study [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

“…Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources are the most frequently chosen for LC-MS analyses. Both of them can offer complementary information; for instance, whereas ESI has been the most commonly used interface for phenols profiling [ 33 , 34 , 36 ], APCI has demonstrated some advantages for the detection of specific families of compounds such as tocopherols or sterols and has also proved to be suitable for phenolic compounds determination [ 39 , 40 , 41 ]. In GC-MS, electron impact (EI) is the most used ionization source because, at 70 eV, it produces a characteristic fragmentation pattern that enables identification of compounds by means of mass spectral library search.…”

Section: Introductionmentioning

confidence: 99%

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Unravelling the Distribution of Secondary Metabolites in Olea europaea L.: Exhaustive Characterization of Eight Olive-Tree Derived Matrices by Complementary Platforms (LC-ESI/APCI-MS and GC-APCI-MS)

et al. 2018

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In order to understand the distribution of the main secondary metabolites found in Olea europaea L., eight different samples (olive leaf, stem, seed, fruit skin and pulp, as well as virgin olive oil, olive oil obtained from stoned and dehydrated fruits and olive seed oil) coming from a Picudo cv. olive tree were analyzed. All the experimental conditions were selected so as to assure the maximum coverage of the metabolome of the samples under study within a single run. The use of LC and GC with high resolution MS (through different ionization sources, ESI and APCI) and the annotation strategies within MetaboScape 3.0 software allowed the identification of around 150 compounds in the profiles, showing great complementarity between the evaluated methodologies. The identified metabolites belonged to different chemical classes: triterpenic acids and dialcohols, tocopherols, sterols, free fatty acids, and several sub-types of phenolic compounds. The suitability of each platform and polarity (negative and positive) to determine each family of metabolites was evaluated in-depth, finding, for instance, that LC-ESI-MS (+) was the most efficient choice to ionize phenolic acids, secoiridoids, flavonoids and lignans and LC-APCI-MS was very appropriate for pentacyclic triterpenic acids (MS (−)) and sterols and tocopherols (MS (+)). Afterwards, a semi-quantitative comparison of the selected matrices was carried out, establishing their typical features (e.g., fruit skin was pointed out as the matrix with the highest relative amounts of phenolic acids, triterpenic compounds and hydroxylated fatty acids, and seed oil was distinctive for its high relative levels of acetoxypinoresinol and tocopherols).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Unravelling the Distribution of Secondary Metabolites in Olea europaea L.: Exhaustive Characterization of Eight Olive-Tree Derived Matrices by Complementary Platforms (LC-ESI/APCI-MS and GC-APCI-MS)

et al. 2018

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“…Calibration curves were constructed using external standards preparing solutions in solvent and considering seven concentration points (2,5,10,20,50,70, 100 μg/mL). Extraction and analysis were carried out according the Materials and Methods section.…”

Section: High-performance Liquid Chromatography Methods Optimization Amentioning

confidence: 99%

“…The healthy properties of extra-virgin olive oil (EVOO), such as anti-inflammatory, anticancer, and neuroprotective, are mainly due to the presence of phenolic compounds (PCs) within the food matrix [1]. An extensive literature concerning the identification and quantification of polyphenols in EVOO has been reported [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Application of deep eutectic solvents for the extraction of phenolic compounds from extra‐virgin olive oil

et al. 2020

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A HPLC-DAD/ESI-MS method has been developed and validated for the analysis of the most representative phenolic compounds in extra-virgin olive oil (EVOO) samples using a green extraction approach based on deep eutectic solvents (DESs) at room temperature. We examined ten DESs based on choline chloride and betaine in combination with different hydrogen bond donors comprising six alcohols, two organic acids, and one urea. Five phenolic compounds, belonging to the classes of secoiridoids and phenolic alcohols, were selected for the evaluation of extraction efficiency. A betaine-based DES with glycerol (molar ratio 1:2) was found to be the most effective for extracting phenolic compounds as compared to a conventional solvent. The optimization of the extraction method involved the study of the quantity of water to be added to the DES and evaluation of the sampleto-solvent ratio optimal condition. Thirty percent of water added to DES and sample to solvent ratio 1:1 (w/v) were selected as the best conditions. The chromatographic method was validated by studying LOD, LOQ, intraday and interday retention time precision, and linearity range. Recovery values obtained spiking seed oil sample aliquots with standard compounds at 5 and 100 μg/g concentration were in the range between 75.2% and 98.7%.

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“…The employed HPLC-PAD-MS methodology allowed the identification of 18 peaks ( Table 3). Six of them, 3,4-Dihydroxyphenylglycol (3,4-DHPG), Hydroxytyrosol characteristics, as well as bibliography data (Bajouba et al, 2016;Michel et al 2015;Jerman Klen, 2014). It should be mentioned that there was other peaks in the chromatogram with spectral characteristics typical for secoiridoids, but their identification and quantitative assessment was not possible because of the lack of reference substances.…”

Section: Identification and Distribution Of Phenolic And Secoiridoid mentioning

confidence: 99%

Olive mill wastewater as a potential source of antibacterial and anti-inflammatory compounds against the food-borne pathogen Campylobacter

Silván

Pinto-Bustillos

Vásquez-Ponce

et al. 2019

Innovative Food Science & Emerging Technologies

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Phenolics and secoiridoids from crude olive mill wastewater (OMW) were purified using a preparative hybrid separation process: pressure-driven cross-flow membrane micro-(MF) and ultrafiltration (UF) and solid phase extraction (SPE). Five fractions with different contents of these compounds were obtained and their antibacterial activity against several strains of Campylobacter was evaluated. The results showed that the fraction with the highest content of phenolic and secoiridoid compounds had a relevant antibacterial activity against all tested strains with a strain-dependent character. Pre-treatment of LPS-stimulated cells with this fraction decreased the production of nitric oxide (NO), showing its potential as anti-inflammatory agent. Finally, the effect of the same fraction on the viability of human intestinal epithelial cell lines was evaluated. In conclusion, OMW could be useful as a potential source of bioactive compounds against Campylobacter, increasing the valorisation of olive oil by-products.

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Comparing two metabolic profiling approaches (liquid chromatography and gas chromatography coupled to mass spectrometry) for extra-virgin olive oil phenolic compounds analysis: A botanical classification perspective

Cited by 73 publications

References 35 publications

Unravelling the Distribution of Secondary Metabolites in Olea europaea L.: Exhaustive Characterization of Eight Olive-Tree Derived Matrices by Complementary Platforms (LC-ESI/APCI-MS and GC-APCI-MS)

Unravelling the Distribution of Secondary Metabolites in Olea europaea L.: Exhaustive Characterization of Eight Olive-Tree Derived Matrices by Complementary Platforms (LC-ESI/APCI-MS and GC-APCI-MS)

Application of deep eutectic solvents for the extraction of phenolic compounds from extra‐virgin olive oil

Olive mill wastewater as a potential source of antibacterial and anti-inflammatory compounds against the food-borne pathogen Campylobacter

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