Comparing Trio-Modified Papanicolaou Staining Methods for Assessing Liquid-Based Cytology Samples

Odigie, Efosa Bolaji; Achukwu, Peter Uwadiegwu

doi:10.5099/aj170300119

Cited by 2 publications

(7 citation statements)

References 9 publications

(31 reference statements)

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“…From the cost of the reagents to the decreased turnaround time and quality of the slides, Rapid PAP has shown maximum advantages compared to conventional PAP staining. In addition, the PAP staining procedures have been modified to save reagent consumption and minimise processing time while improving the staining quality of the slides [6].…”

Section: Discussionmentioning

confidence: 99%

Cytomorphologic Analysis of Wet and Spray Fixation Methods in Oral Exfoliative Cytology

Hulimane

Mulki

2020

ADUM

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For a long time, oral exfoliative cytology (OEC) has been implemented as an effective preliminary diagnostic tool for pathological lesions and various methods for fixation of the cytology specimens have been studied. The present study was undertaken to compare the efficacy between the wet and spray type of fixation methods for Papanicolaou (PAP) stained oral cytosmears. The study comprised of 45 healthy subjects in the age group of 20-25 yrs. For each subject, two smears were collected from the buccal mucosa and subjected to wet and spray fixation methods respectively. Both the smears were stained using a commercial Rapid Pap Kit. Smears were observed microscopically and evaluated for cytomorphological features involving uniformity of staining, cellular morphology, nuclear morphology, cellular staining, nuclear staining and presence of impurities. Comparisons were made between the two methods of fixation and statistically analysed using McNemar nonparametric test. Cells were evenly distributed in wet-fixed smears (n=38, 95%) compared to spray fixed smears (n=19, 47.5%). Wet-fixed smears showed lesser impurities (n=13, 32.5%) than spray fixed smears (n=27, 67.5%). However, other parameters such as cytological and nuclear morphology, staining of cytoplasm and nucleus were found to be not significant when compared between the two methods of fixation (p<0.05). The study shows that wet-fixed smears have better cellular distribution and relatively fewer impurities when compared to the spray fixed smears. The method of wet-fixed smears may be used as an alternative to spray fixed smears. A larger sample size may be required for further validation.

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Section: Discussionmentioning

confidence: 99%

Cytomorphologic Analysis of Wet and Spray Fixation Methods in Oral Exfoliative Cytology

Hulimane

Mulki

2020

ADUM

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“…Hence, n= 4*0.165*0.835/0.0025 amounting to (n=220). However, 113 participants from the street sex worker's business were recruited from the 4 LGAs in Benin City. The reason for the short fall was partly due to the nature of the street sex worker's business as they are always out for monetary gains.…”

Section: Sample Size Determinationmentioning

confidence: 99%

“…"A simple random sampling was undertaken among street sex workers in Benin City, Nigeria following an established method adopted by researchers from similar studies in the locality" [11,13,3]. "The SSWs were surveyed using a structured questionnaire, which was completed on a voluntary basis and conducted in vernacular (Pidgin English) where necessary" [4]. "The interview segment offered predominantly multiple-choice…”

Section: Data Collectionmentioning

confidence: 99%

“…A street FSW is a female prostitute, who stands out at different locations for advertising sexuality for patronage. Resident FSWs, in turn, are women prostitutes that are resident in a particular identifiable brothel or hotel strictly for commercial sex [3,4]. According to Ladipo et al [5] "a brothel is a residential 'quarters' for sex workers habitation, where sex services are obtained or sold.…”

Section: Introductionmentioning

confidence: 99%

“…Before this research, empirical data on cervicitis screening of the street sex workers in Benin City remains completely unavailable while most available studies deal majorly on the global perspectives thereby leaving out the regional and sub-regional areas relatively unexplored. Benin City in Nigeria is one of such area where street sex work is prominent and yet, no effort so far by researchers to looking into this neglected aspect of the social deviancy by the female sex workers [4]. Therefore, there is a need for the screenings of the street sex workers who are occupationally exposed to cervical lesions, which may become cancerous if unawares.…”

Section: Introductionmentioning

confidence: 99%

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Liquid-based Cytology Profile of Street Sex Workers in Benin City, Edo State, Nigeria

Chiedu,

Ogie,

Ifeoma

et al. 2024

Int. STD Res. Rev.

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Aim: To investigate street sex workers in Benin City Edo state Nigeria using liquid-based cytology method. Study Design: Analytical cross-sectional study. Place and Duration of Study: Department of Medical Laboratory Science, Histopathology Sub-Department, University of Benin, Benin City and the Histopathology Laboratory, University of Benin Teaching Hospital (UBTH)., between December 2016 and May 2017. Methodology: This study was conducted in the local government areas that make up the Benin metropolitan City in Edo State. A simple random sampling method was used to recruit the 113 participants, after which the questionnaire was used as the data gathering tool. The liquid-based cytology method was used for sampling following the cervix examination by a gynecological nurse. Data were analyzed using Frequency tables, and factor analysis using Minitab version 17.0 and the statistical significance was set at p < 0.05. Results: From a total of 113 participants within the street sex workers ring in Benin City, acute cervicitis was predominant 7/113(6.2%) closely followed by the chronic 4/113(3.5%). The prevalence of SSWs living with cervicitis in Benin City 11/113(9.7%) revealed that Teenage SSWs were 5/113 (4.4%) and Migrant SSWs 11/113 (9.7%) and were affected by age and duration of sexual practices (p= 0.583; p= 0.204). The prevalence of HPV sera-positivity revealed that 3/113(2.7%) is infected and is largely distributed amongst the age group 21-30years. A hundred percent (100%) of the SSWs agreed to having heard about condoms and have been using it; while regular condom usage was revealed in the HPV sera-negative group amounting to 101/110 (92%) and 2/3(66.7%) amongst the HPV sera-positive group. Conclusion: In conclusion, cervicitis is the only cervical lesion observed in the street sex workers with HPV infection predominantly affecting the age group (21-30years). Also, teenage prostitution is on the increase and HPV infection amongst the street sex workers in Benin City, Nigeria.

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Comparing Trio-Modified Papanicolaou Staining Methods for Assessing Liquid-Based Cytology Samples

Cited by 2 publications

References 9 publications

Cytomorphologic Analysis of Wet and Spray Fixation Methods in Oral Exfoliative Cytology

Cytomorphologic Analysis of Wet and Spray Fixation Methods in Oral Exfoliative Cytology

Liquid-based Cytology Profile of Street Sex Workers in Benin City, Edo State, Nigeria

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