Comparing Tissue PO2 Measurements by Recessed Microelectrode and Phosphorescence Quenching

Buerk, Donald G.; Tsai, Amy G.; Intaglietta, Marcos; Johnson, Paul C.

doi:10.1007/978-1-4615-4863-8_44

Cited by 16 publications

(9 citation statements)

References 31 publications

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“…This has been done for the cat eye (see Shonat et al 14 for comparison with Linsenmeier 2 ), for skeletal muscle, 25 and for tumors growing in window chambers. 26 In each case, oxygen electrodes were inserted into the tissue, whereas the phosphor was in the blood serum.…”

Section: Discussionmentioning

confidence: 99%

Oxygen distribution and vascular injury in the mouse eye measured by phosphorescence-lifetime imaging

Wilson¹,

Vinogradov²,

Grosul³

et al. 2005

Appl. Opt.

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Maps of the oxygen distribution in the retina of the mouse eye were obtained by phosphorescencelifetime imaging. Phosphor dissolved in the blood was excited by modulated light and phosphorescence imaged through microscope optics with an intensified-CCD camera. Phosphorescence lifetimes and oxygen pressures were calculated for each pixel of the images. The resolution was sufficient to permit the detection of anomalies that result in reduced oxygen pressures in individual retinal capillaries. High-resolution maps of oxygen distribution in the retina can provide greater understanding of the role of oxygen and vascular function in diseases of the eye.

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Section: Discussionmentioning

confidence: 99%

Oxygen distribution and vascular injury in the mouse eye measured by phosphorescence-lifetime imaging

Wilson¹,

Vinogradov²,

Grosul³

et al. 2005

Appl. Opt.

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“…The disadvantage of phosphorescence-lifetime imaging is similar to retinal oximetry in that it is a measurement of oxygen levels within the retinal vasculature rather than the retinal tissue itself. It must be noted however, that a number of studies have compared PO 2 measurements between the vasculature (using phosphorescence-lifetime) and a variety of tissues including the retina (using O 2 microelectrodes) and found comparable results, with only slight decreases in PO2 within the tissue [95,108]. The second disadvantage to phosphorescence-lifetime imaging is primarily due to the lack of evidence in animal models of DR.…”

Section: Phosphorescence-lifetime Imagingmentioning

confidence: 98%

Imaging of Hypoxia in Retinal Vascular Disease

Feenstra¹,

Drawnel²,

Jayagopal³

2018

Early Events in Diabetic Retinopathy and Intervention Strategies

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Retinal tissue hypoxia is a key mediator in the pathogenesis of many leading causes of irreversible vision loss, including diabetic retinopathy. Retinal hypoxia in diabetic retinopathy has been shown to drive the production of pro-inflammatory cytokines and pro-angiogenic growth factors. Together, these factors contribute to disease progression by causing unregulated growth of new blood vessels, increased vascular permeability and cell death within the retina. Studies have shown that retinal hypoxia precedes many of the pathologic events that occur during the progression of diabetic retinopathy such as angiopathy, microaneurysms, and capillary dropout. Therefore, early detection of hypoxia in the retinas of diabetic patients could help clinicians identify problems in patients before irreversible damage has occurred. Currently, oxygen sensitive electrodes remain the gold standard for direct measurement of oxygen tension within the retinal tissue; however the procedure is highly invasive and is therefore limited in its applicability towards preclinical models. Less invasive techniques such as retinal oximetry, phosphorescence-lifetime imaging, and hypoxia-sensitive fluorescent probes have shown promising diagnostic value in facilitating detection of oxygen imbalance correlated with neurovascular dysfunction in DR patients. This review highlights the current progress and potential of these minimally invasive hypoxia-imaging techniques in diabetic retinopathy.

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“…Some more recent studies are making use of the quenching effect of oxygen on the emission of phosphorescent dyes [17,[24][25][26][27], using, for example, fiber-optic optodes (Oxylite) in analogy to the aforementioned needle electrodes. These instruments provide an invasive and locally limited assessment of tissue oxygenation down to a certain depth.…”

Section: Introductionmentioning

confidence: 99%

Non-invasive imaging of tissue PO2 in malignant melanoma of the skin

et al. 2006

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In various tumor systems, decreased PO2 values have been demonstrated by various methods. This study addresses the question of whether tumor hypoxia can be found in cutaneous melanoma using lifetime imaging of non-invasive sensors showing phosphorescence quenched by oxygen. Twenty-three cases of cutaneous malignant melanoma (average tumor thickness 1.25 mm, range 0.5-8 mm) were examined using the SkinCam lifetime imaging system for the assessment of cutaneous PO2 levels within the tumors and in adjacent clinically normal skin. For comparison, 30 non-melanoma skin tumors were evaluated. In 15 exploitable melanoma cases, the average hypoxic difference of the lesion compared with the surrounding skin was -10 mmHg, typically associated with an inhomogeneous distribution. Only 10% of the non-melanoma lesions showed a similar hypoxia (false positives). The SkinCam equipment uses a non-invasive imaging method and provides further diagnostic hints in the assessment of benign and malignant skin tumors.

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Comparing Tissue PO2 Measurements by Recessed Microelectrode and Phosphorescence Quenching

Cited by 16 publications

References 31 publications

Oxygen distribution and vascular injury in the mouse eye measured by phosphorescence-lifetime imaging

Oxygen distribution and vascular injury in the mouse eye measured by phosphorescence-lifetime imaging

Imaging of Hypoxia in Retinal Vascular Disease

Non-invasive imaging of tissue PO2 in malignant melanoma of the skin

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