Comparing the level of bystander effect in a couple of tumor and normal cell lines

Soleymanifard, Shokouhozaman; Bahreyni, Mohammad T Toossi

doi:10.4103/0971-6203.94745

Cited by 10 publications

(11 citation statements)

References 14 publications

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“…As in previous studies,[89] we found RIBE in QU-DB and MRC5 cells at doses of 0.5, 2 and 4Gy, the same doses were applied in this study. However, the same study should be performed with very low dose to find whether RIBE signal can be produced, and propagated by bystander cells at environmentally relevant low doses, similar to the present study.…”

Section: Discussionsupporting

confidence: 77%

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MRC5 and QU-DB bystander cells can produce bystander factors and induce radiation bystander effect

et al. 2014

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Radiation damages initiated by radiation-induced bystander effect (RIBE) are not limited to the first or immediate neighbors of the irradiated cells, but the effects have been observed in the cells far from the irradiation site. It has been postulated that bystander cells, by producing bystander factors, are actively involved in the propagation of bystander effect in the regions beyond the initial irradiated site. Current study was planned to test the hypothesis. MRC5 and QU-DB cell lines were irradiated, and successive medium transfer technique was performed to induce bystander effects in two bystander cell groups. Conditioned medium extracted from the target cells was transferred to the bystander cells (first bystander cells). After one hour, conditioned medium was substituted by fresh medium. Two hours later, the fresh medium was transferred to a second group of non-irradiated cells (second bystander cells). Micronucleated cells (MC) were counted to quantify damages induced in the first and second bystander cell groups. Radiation effect was observed in the second bystander cells as well as in the first ones. Statistical analyses revealed that the number of MC in second bystander subgroups was significantly more than the corresponding value observed in control groups, but in most cases it was equal to the number of MC observed in the first bystander cells. MRC5 and QU-DB bystander cells can produce and release bystander signals in the culture medium and affect non-irradiated cells. Therefore, they may contribute to the RIBE propagation.

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Section: Discussionsupporting

confidence: 77%

“…The current study was designed to find out if the experimental results would confirm the proposed hypothesis (second order of RIBE). For this purpose, two cell lines that were able to produce bystander signals and response to them[ 8 ] were chosen. Then successive medium transfer technique was performed to induce RIBE in bystander cells.…”

Section: Introductionmentioning

confidence: 99%

MRC5 and QU-DB bystander cells can produce bystander factors and induce radiation bystander effect

et al. 2014

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“…[10] Therefore, it was concluded that if fractionated irradiation results in the occurrence of RIBE following each fraction, it may also enhance the RIBE level when compared with an equal single dose irradiation. In other words, it is expected to observe a higher RIBE level in 4 Gy FBC (2 × 2 Gy) than in 4 Gy SBC.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the bystander effect in MRC5 cells after acute and fractionated irradiation in vitro

Soleymanifard¹,

Toossi²,

Samani³

et al. 2014

J Med Phys

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Radiation-induced bystander effect (RIBE) has been defined as radiation responses observed in nonirradiated cells. It has been the focus of investigators worldwide due to the deleterious effects it induces in nonirradiated cells. The present study was performed to investigate whether acute or fractionated irradiation will evoke a differential bystander response in MRC5 cells. A normal human cell line (MRC5), and a human lung tumor cell line (QU-DB) were exposed to 0, 1, 2, and 4Gy of single acute or fractionated irradiation of equal fractions with a gap of 6 h. The MRC5 cells were supplemented with the media of irradiated cells and their micronucleus frequency was determined. The micronucleus frequency after single and fractionated irradiation did not vary significantly in the MRC5 cells conditioned with autologous or QU-DB cell-irradiated media, except for 4Gy where the frequency of micronucleated cells was lower in those MRC5 cells cultured in the media of QU-DB-exposed with a single dose of 4Gy. Our study demonstrates that the radiation-induced bystander effect was almost similar after single acute and fractionated exposure in MRC5 cells.

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“…Additionally, different sensitivity of organs leads to different carcinogenesis risks years after exposure [38]. Different effects induced by the bystander effect in different cell types have been observed in in-vitro studies [39-41]. In-vivo studies which have confirmed the expression of carcinogenesis markers in non-targeted tissues is deemed to be different.…”

Section: Introductionmentioning

confidence: 99%

Radiation-induced Non-targeted Effect and Carcinogenesis; Implications in Clinical Radiotherapy

et al. 2018

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Bystander or non-targeted effect is known to be an interesting phenomenon in radiobiology. The genetic consequences of bystander effect on non-irradiated cells have shown that this phenomenon can be considered as one of the most important factors involved in secondary cancer after exposure to ionizing radiation. Every year, millions of people around the world undergo radiotherapy in order to cure different types of cancers. The most crucial aim of radiotherapy is to improve treatment efficiency by reducing early and late effects of exposure to clinical doses of radiation. Secondary cancer induction resulted from exposure to high doses of radiation during treatment can reduce the effectiveness of this modality for cancer treatment. The perception of carcinogenesis risk of bystander effects and factors involved in this phenomenon might help reduce secondary cancer incidence years after radiotherapy. Different modalities such as radiation LET, dose and dose rate, fractionation, types of tissue, gender of patients, etc. may be involved in carcinogenesis risk of bystander effects. Therefore, selecting an appropriate treatment modality may improve cost-effectiveness of radiation therapy as well as the quality of life in survived patients. In this review, we first focus on the carcinogenesis evidence of non-targeted effects in radiotherapy and then review physical and biological factors that may influence the risk of secondary cancer induced by this phenomenon.

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Comparing the level of bystander effect in a couple of tumor and normal cell lines

Cited by 10 publications

References 14 publications

MRC5 and QU-DB bystander cells can produce bystander factors and induce radiation bystander effect

MRC5 and QU-DB bystander cells can produce bystander factors and induce radiation bystander effect

Investigation of the bystander effect in MRC5 cells after acute and fractionated irradiation in vitro

Radiation-induced Non-targeted Effect and Carcinogenesis; Implications in Clinical Radiotherapy

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