Comparing the Histological Structure of the Fat Body and Malpighian Tubules in Different Phases of Honeybees, Apis mellifera jemenatica (Hymenoptera: Apidae)

Aljedani, Dalal Musleh

doi:10.3923/je.2018.114.124

Cited by 8 publications

(8 citation statements)

References 25 publications

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“…This occurrence was observed in 11/20 larvae that also showed changes in the PFB. However, it was seen that transition to the pupal stage in Apis mellifera induces degeneration of the Malpighian tubules [53], which was not recorded in larvae exposed to TiO2 nanoparticles.…”

Section: Discussionmentioning

confidence: 90%

Histopathology of Chironomus riparius (Diptera, Chironomidae) exposed to metal oxide nanoparticles

Stojanović

Milošević

Vitorovic

et al. 2021

ARCH BIOL SCI BELGRA

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As the production of metal-based nanomaterials increases, it is inevitable that nano-scale products and byproducts will enter the aquatic environment. In terms of global production, the most abundant nano-oxides are TiO2, CeO2 and Fe3O4 nanoparticles. Chironomus riparius is commonly used for ecotoxicological assessment and defining its histopathological biomarkers that showcase the toxic effect of tested nanoparticles should lead to a better understanding of the consequences of nanomaterial accumulation in aquatic ecosystems. In this study, a histological description of the digestive and excretory systems as well as the fat body structure of C. riparius larvae is provided. In addition, potential histological biomarkers of nano-oxide toxicity were determined based on the obtained histopathological alterations in organs. Vacuolization was observed in epithelial cells of midgut region I that were treated with nano-Fe3O4 as well as in Malpighian tubules treated with nano-Fe3O4 and nano-CeO2. Larvae exposed to nano-TiO2 showed alterations in the fat body and midgut region II tissue architecture. Additionally, shortening of the intestinal brush border was determined in groups exposed to nano-Fe3O4. These results reveal the high sensitivity of these organs, which can be used as biomarkers in histopathological assessment and therefore lead to further improvement of existing methodology in ecotoxicological studies.

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Section: Discussionmentioning

confidence: 90%

Histopathology of Chironomus riparius (Diptera, Chironomidae) exposed to metal oxide nanoparticles

Stojanović

Milošević

Vitorovic

et al. 2021

ARCH BIOL SCI BELGRA

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“…Крім того, це орган біосинтетичної та метаболічної активності (Arrese & Soulages, 2010). Як у передімагінальний період, так і в дорослій фазах жирове тіло бере участь у накопиченні токсичних матеріалів, таких як урати (Dalal & Aljedani, 2018). Забезпечення енергією вважається основним напрямком ліпідного обміну, Особливо гостро ці процеси відбуваються у періоди стресових ситуацій, наприклад, відсутності корму (Keller et al, 2005).…”

Section: вступunclassified

Features of fat body development in the body of honey bees

Kovalskyi,

Zhmur

2024

SMLNUVMBA

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The fat body is considered an organ that contains a conglomerate of proteins and fats and acts similar to the liver of mammals. It is loose tissue that is involved in some metabolic functions. One of these functions is to store and release energy in response to energy needs. The article presents data on the development of trophocytes of the fat body of the honey bee and factors that affect the process of lipolysis and lipogenesis. These processes are related to the technological methods of keeping bee colonies. The purpose of the work was to study patterns and factors that affect the growth and development of the structural elements of the fat body. For this, honeycombs with sealed brood were taken from the nest for incubation. After leaving the cells, the selected bees were placed in the nest of the bee colony. Marked bees were selected every 3 days. Histological preparations of various parts of the fat body were studied up to 21 days inclusive. According to research, trophocytes differ from enocytes in their larger size. These rounded cells have a distinct membrane and granular cytoplasm. In the cytoplasm, mostly oval-shaped vacuoles were found, which are filled with energetic and plastic substances, while others are empty. The age-related dynamics of the length of fat body trophocytes was studied. It was found that at the exit from the cell, they are the smallest and amount to an average of 50.12 ± 3.18 μm. With an increase in the activity of enzymes of the intestinal tract, an increase in the morphometric indicators of fat cells is observed. The maximum indicators of the length of trophocytes of the pericardial zone of the fat body were found in 15 daily bees. Measurement of the length of fat cells during this period shows that compared to diurnal bees, trophocytes are larger by 34.6 % (Р < 0.001). After 14 days, a gradual regression of the morphometric indicators of trophocytes is noted. The article analyzes the role of the fat body in the life of the honey bee.

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“…У процесі еволюції бджоли стали будувати комірки стільника тково з воску (Woyke et al, 2012;Kovalskyi et al, 2021). Будівництво стільників і потенційні можливості бджіл із восковиділення пов'язані з фізіологічним станом восковидільних залоз (Dalal & Aljedani, 2018;Strachecka et al, 2021). Віск є другим важливим продуктом після меду, який отримують від медоносних бджіл.…”

Section: вступunclassified

Improvement of honey bees for intensification of wax production

Kovalskyi,

Kovalska,

Druzhbiak

et al. 2023

SMLNUVMBA

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Obtaining the maximum amount of wax from honey bees is the basis of beekeeping technological processes. However, the intensification of its production leads to partial exhaustion of the honey bee's body. Therefore, today the question of researching factors that will contribute to their body's recovery is relevant. The article provides data on the influence of the strength of colonies, the type, and the amount of feed on the body's functional state of honey bees when obtaining wax. The obtained research results indicate that wax secretion is proportionally dependent on the presence in nature and the intensity of carbohydrate (nectar, sugar syrup) and protein (pollen) food entering the nest. As daily nectar intake increases for every 250 g, the wax productivity of a 700 g family increases by an average of 43.18 g (from 35.62 to 54.14 g). When receiving pollen in the amount of 500–550 g, wax secretion is 190–210 g. In the complete absence of honey collection, wax secretion in bees stops. It was established that a large amount of honeycombed honey practically does not affect the bees' wax secretion. At the same level of food supply, with an increase in the strength of the family, its total wax secretion increases due to an increase in the number of wax-producing workers. From colonies weighing 1.5 and 4.5 kg, 667.05 and 1484.55 g of wax were obtained, respectively. At the same time, the individual wax-secreting activity, which is calculated per unit mass of workers, decreases from 444.7 g to 329.9 g of wax/1 kg of bees. This is due to a decrease in the nutrition of wax-producing individuals, as the number of bees increases with the same intensity of carbohydrate food entering the nest. It was also established that the amount of wax released simultaneously is proportional to the number of young bees in the colonies.

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Comparing the Histological Structure of the Fat Body and Malpighian Tubules in Different Phases of Honeybees, Apis mellifera jemenatica (Hymenoptera: Apidae)

Cited by 8 publications

References 25 publications

Histopathology of Chironomus riparius (Diptera, Chironomidae) exposed to metal oxide nanoparticles

Histopathology of Chironomus riparius (Diptera, Chironomidae) exposed to metal oxide nanoparticles

Features of fat body development in the body of honey bees

Improvement of honey bees for intensification of wax production

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