Comparing the genetic diversity of ORF30 of Australian isolates of 3 equid alphaherpesviruses

Cuxson, Jennifer L; Hartley, Carol A.; Ficorilli, Nino; Symes, S.J.; Devlin, Joanne M.; Gilkerson, James R.

doi:10.1016/j.vetmic.2013.12.007

Cited by 20 publications

(25 citation statements)

References 29 publications

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“…A single amino acid substitution from asparagine (N) to aspartic acid (D) at position 752 has been associated with increased neurovirulence [10, 12]. However, this relationship is not observed for all EHV-1 field viruses, as is exemplified by the fact that in a recent Australian study, four out of five archival EHV-1 isolates from EHM cases belonged to the ORF30 N 752 genotype [13]. Hence, it is likely that the viral markers of virulence are more complex than this single amino acid substitution [5, 14].…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of equid herpesvirus type 1 from cases of abortion in Poland

et al. 2017

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Equid herpesvirus type 1 (EHV-1) is a common viral infection associated with varied clinical outcomes including respiratory disease, abortion and neurological disease. We have characterized EHV-1 sequences (n = 38) obtained from cases of equine abortion in Poland between 1999 and 2016, based on sequencing of PCR products from open reading frames (ORF) 30 and 68 of the EHV-1 genome. The majority (81.6%) of sequences were not classified into any of the previously described groups based on the ORF68 sequence. The remaining sequences belonged to ORF68 group III (7.9%) or IV (10.5%). A haplotype network analysis did not show any obvious structure within networks of local Polish sequences, nor within a global network of 215 EHV-1 sequences when these networks were coloured based on the geographical origin of viruses or date of detection. Our data suggest that ORF68 does not provide a reliable molecular marker for epidemiological studies of EHV-1, at least in a global sense. Its usefulness to aid local investigations of individual outbreaks remains to be established. All but two Polish EHV-1 sequences belonged to the ORF30 N752 genotype. The two ORF30 D752 viruses were obtained from abortion cases in 2009 and 2010. Hence, abortion cases that occurred in Poland between 1999 and 2016 were caused predominantly by EHV-1 with the ORF30 N752 genotype, with no indication of an increase in the prevalence of the ORF30 D752 variant.Electronic supplementary materialThe online version of this article (doi:10.1007/s00705-017-3376-3) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Genetic characterization of equid herpesvirus type 1 from cases of abortion in Poland

et al. 2017

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“…The prevalence of neuropathogenic strains in abortion outbreaks varies between 1.5% and 25.8%. The percentage prevalence was highest (25.8%) in France (Pronost et al, 2010) followed by 19.4% in U.S.A (Smith et al, 2010), 10.6% in Germany (Fritsche & Borchers, 2011), 7% in Argentina (Vissani et al, 2009), 3.1% in Poland (Stasiak et al, 2015), 2.7% in Japan (Tsujimura et al, 2011) and 1.5% in Australia (Cuxson et al, 2014).…”

Section: Equine Herpesvirus Myeloencephalopathymentioning

confidence: 94%

“…outbreaks of EHV1 infection by neuropathogenic strains of EHV1. The incidence of neuropathogenic genotype from cases of neurological illness reported from different countries varies between 20% and 86% (Perkins et al, 2009;Vissani et al, 2009;Pronost et al, 2010;Fritsche & Borchers, 2011;Cuxson et al, 2014). The prevalence of neuropathogenic strains in abortion outbreaks varies between 1.5% and 25.8%.…”

Section: Equine Herpesvirus Myeloencephalopathymentioning

confidence: 99%

“…Europian countries viz., France (Pronost et al, 2010;van Galen et al, 2015), Germany (Fritsche & Borchers, 2011;Damiani et al, 2014), Belgium (van der Meulen et al, 2003;Gryspeerdt et al, 2011), Poland (Stasiak et al, 2015), Netherlands (Goehring et al, 2006) and Croatia (Barbic et al, 2012); North American countries viz., Canada (Burgess et al, 2012) and U.S.A (Nugent et al, 2006;Henninger et al, 2007;Perkins et al, 2009;Smith et al, 2010;Pusterla et al, 2012); South American countries viz., Brazil (Mori et al, 2011) and Argentina (Vissani et al, 2009); Asian countries viz., Turkey (Yilmaz et al, 2012); Japan (Tsujimura et al, 2011) and India (Unpublished data); Islands viz ., Australia (Cuxson et al, 2014) and Newzealand (McFadden et al, 2016); African countries viz., Ethiopia (Negussie et al, 2015) experienced…”

Section: Prevalence Of Neuropathogenictymentioning

confidence: 99%

“…At the same time, there has been rise in incidence of EHM in many parts of world viz., Europe, North America, South America, Africa and Oceania (Perkins et al, 2009;Vissani et al, 2009;Pronost et al, 2010;Smith et al, 2010;Fritsche & Borchers 2011;Tsujimura et al, 2011, Cuxson et al, 2014Negussie et al, 2015;McFadden et al, 2016). The neuropathogenic strains of EHV1 (causing EHM) have also been reported from Asian countries such as Japan (Tsujimura et al, 2011) and India (Unpublished data).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Emergence of equine herpes virus 1 myeloencephalopathy: A brief review

Gulati¹,

Anagha²,

Riyesh³

et al. 2016

JEBAS

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Genetic characterization of equine herpesvirus 1 isolates from abortion outbreaks in India

et al. 2016

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Equine herpesvirus 1 (EHV1) is a common pathogen of horses that causes upper respiratory tract disease, abortion, neonatal death and neurological disease. The neurological form of disease is called equine herpesvirus myeloencephalopathy (EHM). During the past decade, the incidence of EHM has been on the rise in Europe, North America, Australia and Asia. Some EHV1 isolates causing EHM exhibit a single-nucleotide polymorphism (SNP) in the DNA polymerase gene (ORF30) at position 2254 (A to G). Further, based on polymorphism in the ORF68, EHV1 isolates have been classified into different groups. The aim of the present study was to estimate the genetic diversity of EHV1 and to determine the prevalence of the neuropathogenic genotype of EHV1 in India. Out of 133 clinical specimens from abortion cases in northern India, 56 were positive for EHV1 infection. Analysis of the A/G SNP by real-time PCR and sequence analysis revealed that 54 of 56 samples (96.43 %) were of the non-neuropathogenic genotype (A), while two (3.57 %) had the neuropathogenic marker (G). Sequence analysis of the polymorphic region of ORF68 of EHV1 isolates (n = 9) from India indicated that the Delhi/1998, Tohana-2/2013, Hisar-2/2014 and Hisar-15/1990 isolates belonged to group 4, while the Jind/1996, Rajasthan/1998, Delhi-3/2007 and Tohana-5/1996 isolates clustered within group 5. One isolate (Hisar-7/1990) exhibited SNPs at positions C and C, forming a separate group. Here, we report for the first time the detection of neuropathogenic genotypes of EHV1 in India and show that Indian EHV1 isolates cluster within groups 4 and 5.

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Comparing the genetic diversity of ORF30 of Australian isolates of 3 equid alphaherpesviruses

Cited by 20 publications

References 29 publications

Genetic characterization of equid herpesvirus type 1 from cases of abortion in Poland

Genetic characterization of equid herpesvirus type 1 from cases of abortion in Poland

Emergence of equine herpes virus 1 myeloencephalopathy: A brief review

Genetic characterization of equine herpesvirus 1 isolates from abortion outbreaks in India

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