Comparing the Effects of Benzyladenine and &lt;i&gt;meta&lt;/i&gt;-Topolin on Sweet Basil (&lt;i&gt;Ocimum  basilicum&lt;/i&gt;) Micropropagation

Kőszeghi, Szidónia; Bereczki, Csaba; Balog, Adalbert; Benedek, Klára

doi:10.15835/nsb649464

Cited by 8 publications

(5 citation statements)

References 19 publications

(33 reference statements)

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“…For two species of the Prunus genus, cultures grown on medium with a hydroxylated benzylaminopurine derivative had twice as much fresh weight than those on a medium supplemented with BAP [27]. Although the effect of m-T on the propagation of sage species has not been described, it was found to stimulate the proliferation of two other species of Lamiaceae, Ocimum basilicum [28] and Dracocephalum forrestii, [29] and improve the morphology of the shoot culture. Of three concentrations of m-T used in our study, 1 mg/L was found to be most effective.…”

Section: Discussionmentioning

confidence: 96%

The Stimulatory Effect of Purine-Type Cytokinins on Proliferation and Polyphenolic Compound Accumulation in Shoot Culture of Salvia viridis

et al. 2020

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The present study demonstrates hormonal control of Salvia viridis growth and development using four different purine-type cytokinins at different concentrations. The addition of cytokinins significantly increased biomass of cultures, proliferation rate, and, interestingly, secondary metabolite production. The best response in terms of multiplication ratio was recorded on Murashige and Skoog medium supplemented with 0.5 mg/L BPA (N-benzylotetrahydropyranyl adenine), while the greatest biomass accumulation was achieved when supplemented with 1 mg/L m-T (meta-topoline). Quantitative UPLC-DAD analysis of the hydromethanolic extract from S. viridis culture revealed the presence of 12 polyphenols: seven phenolic acids and five phenylethanoids. The highest total content of polyphenolic compounds was found in shoots cultivated on medium with 2 mg/L BPA (18.66 mg/g DW): almost twice that of control shoots. The medium was also the most optimal for the biosynthesis of rosmarinic acid, the predominant phenolic acid. However, the greater phenylethanoid accumulation was stimulated by 1 mg/L m-T: the metabolite content was above three times higher than that found in shoots grown on the control medium (8.03 mg/g DW vs. 2.37 mg/g DW). Hence, it was demonstrated that phytohormones are capable of influencing not only vital physiological processes, but therapeutic potential of plants as well. Therefore, the cytokinin-based sage cultures may be also considered as the alternative sources of bioactive compounds.

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Section: Discussionmentioning

confidence: 96%

The Stimulatory Effect of Purine-Type Cytokinins on Proliferation and Polyphenolic Compound Accumulation in Shoot Culture of Salvia viridis

et al. 2020

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“…BA is the most commonly used cytokinin for micropropagation, but plant taxa differ in their preference towards specific cytokinins. Recently, meta-Topolin (mT) has become a more common cytokinin in the in vitro plant production (Werbrouck et al 1996;Gentile et al 2014;Koszeghi et al 2014). In certain species gibberellic acid (GA 3 ) may also induce the formation of axillary shoots (George et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Optimizing micropropagation conditions for a recalcitrant ninebark (Physocarpus opulifolius L. maxim.) cultivar

Jagiełło-Kubiec

Nowakowska

Ilczuk

et al. 2021

In Vitro Cell.Dev.Biol.-Plant

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Ninebark is a very popular ornamental shrub. Micropropagation is an efficient method for mass production of uniform plant material. This study was designed to develop and optimize conditions at all phases of ninebark micropropagation. For the multiplication stage, the Murashige and Skoog (MS) medium at full concentration and pH 5.8 was chosen as the basal medium. Sorbitol proved a more effective carbohydrate source than fructose, with no adverse effects on shoot vitrification or the medium itself. The best shoot production, both in number and length, was on the medium enriched with 2 and 3 mg·L−1 zeatin. High numbers of shoots were also obtained in treatments with 1 mg·L−1 6-benzyladenine (BA) or 2 mg·L−1 meta-Topolin (mT) in the basal medium. BA was the most cost-effective cytokinin. There was a positive effect of the gibberellic acid on proliferation: the highest shoot number per explant was produced in the presence of 1 mg·L−1 GA3. No effect of the culture age (up to 20 subcultures) on the percentage of regenerating explants was evident, and the highest numbers of shoots were obtained between passages 10 and 17. For rooting, the MS medium at half strength was used. The best rooting was at 1 mg·L−1 IBA. Spraying the in vitro rooted cuttings with abscisic acid (ABA) favored plant acclimation to the ex vitro conditions. Exvitro rooting, including the treatments with IBA and ABA, shortened the production time by approximately one third.

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“…The addition of 8.2 µM mT had a positive effect-the percentage of regenerated plants was higher than of those growing with 8.2 µM BA [36]. Kőszeghi et al [13] compared the effects of the mT and benzyladenine on shoot regeneration in common basil (Olicimum basilicum). Out of the five variants, each with a different concentration of mT in the MS medium, that using 1 mg•L −1 produced the highest number of shoots.…”

Section: Discussionmentioning

confidence: 99%

“…Growth regulators commonly used in in vitro plant propagation are cytokinins. They are applied during the proliferation stage to produce as many axillary buds, and hence microcuttings, as possible [13]. Cytokinins play an important role in regulating different aspects of plant growth and development: they stimulate cell divisions, delay aging of leaves and flower development, define defensive mechanisms helping the plant to adapt to different stressors [14].…”

Section: Introductionmentioning

confidence: 99%

“…It has been shown that topolins increase shoot proliferation, maintain the hormonal/histogenic stability, and improve rooting effectiveness [19]. Using mT instead of benzyladenine (BA) usually generates plants of higher quality, such as with better coloring [20], and reduces the risk of various types of deformations that do occurring during in vitro propagation [13], Kőszeghi et al [13] who used mT in the propagation of basil (Ocimum basilicum L.) and produced fewer deformed plants than with BA.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the Effect of Meta-Topolin and Benzyladenine during Daphne mezereum L. Micropropagation

Nowakowska

Pacholczak

2020

Agronomy

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Meta-Topolin (mT) is one of the lesser-known and only recently discovered cytokinins that can be used as an alternative to the commonly used benzyladenine (BA). The experiment aimed to compare the effects of BA and mT on the regeneration from explants of Daphne mezereum, on the levels of several chemical components, the catalase activity, and to assess genetic diversity of microcuttings. The Murashige and Skoog medium (MS) and the woody plant medium (WPM) were used, enhanced with auxin and 1 mg·L−1 mT or 1 mg·L−1 BA. The results suggest that mT has a more positive effect on shoot regeneration. It also positively affected the levels of various compounds which are generally taken as indicative of micro cutting conditions. The levels of chlorophyll and carbohydrates were the highest in microcuttings from the WPM with 0.1 mg·L−1 NAA. Total soluble proteins and amino acids were the highest in plants from the MS medium with 1 mg·L−1 mT. Increased levels of hydrogen peroxide and catalase activity were observed on media with 1 mg·L−1 BA. Screening with 30 randomly amplified polymorphic DNAs (RAPD) and 20 inter-simple sequence repeat (ISSR) primers demonstrated a considerable level of genetic variation among regenerating plants. This variation is attributed to the condition of the starting material (seed propagated); there were no indications that the two cytokinins tested contributed to increased somaclonal variation.

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Comparing the Effects of Benzyladenine and <i>meta</i>-Topolin on Sweet Basil (<i>Ocimum basilicum</i>) Micropropagation

Cited by 8 publications

References 19 publications

The Stimulatory Effect of Purine-Type Cytokinins on Proliferation and Polyphenolic Compound Accumulation in Shoot Culture of Salvia viridis

The Stimulatory Effect of Purine-Type Cytokinins on Proliferation and Polyphenolic Compound Accumulation in Shoot Culture of Salvia viridis

Optimizing micropropagation conditions for a recalcitrant ninebark (Physocarpus opulifolius L. maxim.) cultivar

Comparison of the Effect of Meta-Topolin and Benzyladenine during Daphne mezereum L. Micropropagation

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