2008
DOI: 10.1002/jmv.21298
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Comparing the antibody responses against recombinant hemagglutinin proteins of avian influenza A (H5N1) virus expressed in insect cells and bacteria

Abstract: The hemagglutinin (HA) of influenza A virus plays an essential role in mediating the entry of the virus into host cells. Here, recombinant full-length HA5 protein from a H5N1 isolate (A/ chicken/hatay/2004(H5N1)) was expressed and purified from the baculovirus-insect cell system. As expected, full-length HA5 elicits strong neutralizing antibodies, as evaluated in microneutralization tests using HA5 pseudotyped lentiviral particles. In addition, two fragments of HA5 were expressed in bacteria and the N-terminal… Show more

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Cited by 37 publications
(41 citation statements)
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References 24 publications
(25 reference statements)
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“…1). Previous reports on the production of recombinant HA in mammalian cells, insect cells, or bacterial systems did not provide information on the presence and function of oligomers versus monomeric forms of HA (1,14,17,19,20,25,27). More recent publications emphasized the importance of high-MW oligomers for optimal immunogenicity of influenza virus recombinant HA proteins.…”
Section: Discussionmentioning
confidence: 99%
“…1). Previous reports on the production of recombinant HA in mammalian cells, insect cells, or bacterial systems did not provide information on the presence and function of oligomers versus monomeric forms of HA (1,14,17,19,20,25,27). More recent publications emphasized the importance of high-MW oligomers for optimal immunogenicity of influenza virus recombinant HA proteins.…”
Section: Discussionmentioning
confidence: 99%
“…Several single component influenza H5 virus vaccines have been developed using rHA proteins produced in mammalian, insect and bacterial expression systems. Early test results of these candidate vaccines in animals [27, 28] and in humans are encouraging; however, as shown by Treanor et al [29] even the highest dose (90 μg) of baculovirus expressed H5 rHA induced a putative protective antibody titer in only 52% of human subjects. Such a vaccine, although well tolerated, is sub-optimal against a pandemic because of the high dosage required and modest immunogenicity.…”
Section: Discussionmentioning
confidence: 99%
“…Such a vaccine, although well tolerated, is sub-optimal against a pandemic because of the high dosage required and modest immunogenicity. Shen et al [27] compared the neutralizing antibody response against the N-terminal rHA ectodomain portion (truncated before the HA1/HA2 polybasic cleavage site) produced in bacteria with the full-length rHA of H5N1 virus produced in insect cells. The results of this study indicate that the nonglycosylated HA produced in bacteria elicited neutralizing titers only four times lower than the glycosylated protein expressed in insect cells.…”
Section: Discussionmentioning
confidence: 99%
“…Vaccines utilizing the expression of recombinant HA in insect, yeast, plant, and mammalian cells are under development and/or in clinical trials (13,23,40,47,51). The main challenge to the recombinant technology is to ensure that the HA products resemble the native virion-associated trimeric spike proteins and can elicit robust immune responses targeting protective conformational epitopes in the globular domain of HA (39,44).…”
mentioning
confidence: 99%