Comparing Techniques for Quantifying Haloxyfop Absorption and Translocation in Johnsongrass (Sorghum halepense)

Abstract

In comparing laboratory techniques, absorption and translocation of14C-haloxyfop was not affected by droplet size or treatment area. Treatment with haloxyfop prior to application of14C-haloxyfop resulted in 27% less translocation of radiolabel from the treated leaf and 43% less acropetal translocation compared with plants receiving only the radiolabeled herbicide. Leaf washing with distilled water, distilled water plus haloxyfop, distilled water plus surfactant, distilled water plus haloxyfop plus surfactant, 100% ethanol, 100% methanol, or methanol:water (1:1 v/v) removed similar amounts of radiolabel, while chloroform removed more. Considering some of the differences found with these techniques, pretreatment with nonlabeled herbicide should be routine practice and water used as the standard solvent.