Comparing reactive oxygen species and DNA fragmentation in semen samples of unexplained infertile and healthy fertile men

Zandieh, Zahra; Vatannejad, Akram; Doosti, Mahmood; Zabihzadeh, Sara; Haddadi, Mohammad; Bajelan, L; Rashidi, Batool Hossein; Amanpour, Saeid

doi:10.1007/s11845-017-1708-7

Cited by 43 publications

(38 citation statements)

References 22 publications

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“…Oxidative stress mediated DNA damage can be either directly because of oxidation of DNA base pairs, breaks in the DNA, and chromatin decondensation, or indirectly as a result of activating caspases and endonucleases. A strong correlation exists between high levels of ROS and SDF in infertile men [49,50]. Ultimately, SDF has an adverse effect on the fertilizing ability of spermatozoa.…”

Section: Discussionmentioning

confidence: 98%

Efficacy of Antioxidant Supplementation on Conventional and Advanced Sperm Function Tests in Patients with Idiopathic Male Infertility

et al. 2020

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Antioxidants are used in the empirical treatment of infertile men. The aim of this study was to evaluate the effects of antioxidant therapy on conventional semen parameters and advanced sperm function tests in men seeking fertility treatment. A total of 148 infertile men of unknown etiology were divided into idiopathic (n = 119) and unexplained male infertility (UMI; n = 29). All participants were treated with the antioxidant supplement 'FH PRO for Men' for a period of three months. Compared with pretreatment results, there was a significant improvement in conventional semen parameters including sperm concentration, total and progressive motility and normal morphology, and seminal oxidation reduction potential (ORP), and sperm DNA fragmentation (SDF) in idiopathic infertile men. The changes were more prominent in idiopathic infertile men positive for ORP and SDF. UMI patients showed an improvement in progressive motility, ORP, and SDF after antioxidant treatment. Statistical analysis revealed that the efficacy of FH PRO for Men was significant in idiopathic male infertility compared with UMI. Treatment of idiopathic male infertility patients with the FH PRO for Men antioxidant regimen for three months resulted in a significant improvement in conventional semen parameters and sperm function. Therefore, FH PRO for Men offers promise for the medical treatment of idiopathic male infertility.

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Section: Discussionmentioning

confidence: 98%

Efficacy of Antioxidant Supplementation on Conventional and Advanced Sperm Function Tests in Patients with Idiopathic Male Infertility

et al. 2020

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“…Some studies praise the SCSA for their prognostic value (Evenson, ; Evenson et al, ) while others do the same for the SCD (Comhaire, Messiaen, & Decleer, ; Fernández, Cajigal, López‐Fernández, & Gosálvez, ; Pregl Breznik, Kovačič, & Vlaisavljević, ; Zheng et al, ). Caution has been recommended when considering prognosis after IVF or ICSI using solely DNA fragmentation (Anifandis et al, ; Cissen et al, ; Erenpreiss, Spano, Erenpreisa, Bungum, & Giwercman, ; Zhang et al, ) particularly when colloid gradients are used since other methods for sperm selection are more valuable, as swim‐up (Zandieh et al, ).…”

Section: Discussionmentioning

confidence: 99%

Diagnostics of DNA fragmentation in human spermatozoa: Are sperm chromatin structure analysis and sperm chromatin dispersion tests (SCD‐HaloSpermG2 ^® ) comparable?

et al. 2019

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Men affected with idiopathic infertility often display basic spermiogramme values similar to fertile individuals, questioning the diagnostic impact of the World Health Organization (WHO) thresholds used. This study explored sperm DNA fragmentation in single ejaculates from 14 fertile donors and 42 patients with idiopathic infertility providing semen for assisted reproductive techniques in a university fertility clinic. Each ejaculate was simultaneously studied for sperm DNA fragmentation by the flow cytometer‐based sperm chromatin structure analysis (SCSA) and the new light‐microscopy‐based sperm chromatin dispersion assay (SCD‐HaloSpermG2®), before and after sperm selection for in vitro fertilisation with a colloid discontinuous gradient. The WHO semen variables did not differ between groups, but DNA fragmentation after SCSA (DFI) or SCD (SDF) was significantly (p < 0.05) higher in patients (DFI: 40.2% ± 3.0 vs. SDF: 40.3% ± 1.4) than in fertile donors (DFI: 17.1% ± 2.1 vs. SDF: 20.9% ± 2.5). Sperm selection led to lower proportions of DNA‐fragmented spermatozoa (DFI: 11.9 ± 1.7 vs. SCD: 10.0 ± 0.9, p < 0.05). The techniques output correlated highly and significantly (r2 = 0.82). DNA fragmentation is confirmed as a relevant variable for scrutinising patients with idiopathic infertility, beyond the evidently insufficient WHO semen analyses. Since both techniques yielded similar results, the reduced necessity of complex equipment when running SCD ought to be considered for a clinical setting.

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“…Чрезмерное количество активных форм кислорода и снижение антиоксидантной способности в процессе старения могут вызывать апоптоз или окислительное повреждение ДНК [18]. Выявлена положительная корреляция между уровнем H 2 O 2 и уровнем фрагментации ДНК сперматозоидов у пациентов с бесплодием [19,20].…”

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Sperm DNA fragmentation in men of different age

Хаят

Брагина

Arifulin

et al. 2019

Androl. genit. hir.

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The study objective is to analyze the content of spermatozoa with single and double-stranded DNA breaks in different age groups.Materials and methods. The level of DNA fragmentation was studied in 300 ejaculate samples obtained from 266 sub- or infertile men. The group 1 included 150 samples obtained from 131 patients under the age of 45 (21–44 years), the group 2 included 150 samples obtained from 135 patients above the age of 45 (45–68 years). Mean ages were 34.8 ± 3.9 and 48.6 ± 3.1 years, respectively. The number of sperm with fragmented DNA was evaluated using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method on ejaculate smears. The number of spermatozoa with >15 % of fragmented DNA was considered elevated. Standard semen analysis was performed in 117 and 97 men from the groups 1 and 2, respectively.Results. The number of sperm with fragmented DNA varied in ejaculated samples from 1.5 to 64.5 %. Mean number of sperm with DNA breaks in the group 1 (12.0 ± 6.0 %) was significantly lower than in the group 2 (16.1 ± 8.3 %, p <0.05). Mean sperm count in the ejaculate of the group 1 (267.0 ± 198.7 million) was significantly higher than in the group 2 (201.0 ± 162.9 million, p = 0.02).Conclusion. We revealed that in men over the age of 45 years, the percentage of spermatozoa with DNA fragmentation is higher than in men under 45 years of age, it may indirectly indicate an increased level of reactive oxygen species in the seminal plasma in older patients.

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Comparing reactive oxygen species and DNA fragmentation in semen samples of unexplained infertile and healthy fertile men

Abstract: The higher levels of intracellular ROS and DNA fragmentation in the semen samples of unexplained infertile couples and their causes might be considered as an important factor related to diagnosis and treatment of the unexplained infertile couples.

Cited by 43 publications

References 22 publications

Efficacy of Antioxidant Supplementation on Conventional and Advanced Sperm Function Tests in Patients with Idiopathic Male Infertility

Efficacy of Antioxidant Supplementation on Conventional and Advanced Sperm Function Tests in Patients with Idiopathic Male Infertility

Diagnostics of DNA fragmentation in human spermatozoa: Are sperm chromatin structure analysis and sperm chromatin dispersion tests (SCD‐HaloSpermG2 ^® ) comparable?

Sperm DNA fragmentation in men of different age

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Comparing reactive oxygen species and DNA fragmentation in semen samples of unexplained infertile and healthy fertile men

Abstract: The higher levels of intracellular ROS and DNA fragmentation in the semen samples of unexplained infertile couples and their causes might be considered as an important factor related to diagnosis and treatment of the unexplained infertile couples.

Cited by 43 publications

References 22 publications

Efficacy of Antioxidant Supplementation on Conventional and Advanced Sperm Function Tests in Patients with Idiopathic Male Infertility

Efficacy of Antioxidant Supplementation on Conventional and Advanced Sperm Function Tests in Patients with Idiopathic Male Infertility

Diagnostics of DNA fragmentation in human spermatozoa: Are sperm chromatin structure analysis and sperm chromatin dispersion tests (SCD‐HaloSpermG2 ® ) comparable?

Sperm DNA fragmentation in men of different age

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Diagnostics of DNA fragmentation in human spermatozoa: Are sperm chromatin structure analysis and sperm chromatin dispersion tests (SCD‐HaloSpermG2 ^® ) comparable?