Comparing label free electrochemical impedimetric and capacitive biosensing architectures

Disposable integrated bismuth citrate-modified screen-printed immunosensor for ultrasensitive quantum dot-based electrochemical assay of C-reactive protein in human serum

“…Moreover, the LOD of the proposed voltammetric assay was lower than a fluoroimmunoassay employing QD labels [6]. In addition, the developed immunosensor presents about one to three orders of magnitude lower LOD than the majority of other electrochemical CRP assays at the expense of longer time of analysis [8][9][10][12][13][14]. Only two electrochemical CRP assays exhibit lower LODs [7,11] but these applications are based on an immunoassay step on microbeads and a M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 14 subsequent amperometric detection step at a spatially separated gold electrode; the microbeads are transferred to the transducer by means of flow [7] or a magnetic field [11].…”

“…However, these approaches require costly instrumentation and their sensitivity is low often result in a lengthy turnaround time. Surface plasmon resonance [3,4], piezoelectric [5], fluorescence [6], and electrochemical techniques [7][8][9][10][11][12][13][14] have also been developed during the past few years for the quantification of CRP. Among these techniques, electrochemical immunoassays have M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 3 received much attention thanks to their high sensitivity, flexibility and low−cost.…”

“…Among these techniques, electrochemical immunoassays have M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 3 received much attention thanks to their high sensitivity, flexibility and low−cost. The majority of the existing electrochemical CRP assays involves capacitive or impedimetric immunosensors, which, in spite of their analytical simplicity (label−free formats), suffer from poor reproducibility and, thus, their use in routine analysis is restricted [12][13][14].…”

An impedimetric biosensor to test neat serum for dengue diagnosis

“…It has been reported that the use of EIS in faradaic impedance measurements of classical antigen-antibody binding events shows good reproducibility and sensitivity [13][14][15]. In this paper, we developed an impedimetric immunosensor based on a modified anti-NS1 gold electrode that is able to detect the NS1 dengue biomarker at interesting clinically relevant levels in buffer as well as in neat serum.…”

Glycoprofiling as a novel tool in serological assays of systemic sclerosis: A comparative study with three bioanalytical methods

“…One of the most sensitive biosensing detection platform is electrochemical impedance spectroscopy (EIS), offering robust, extremely sensitive method for monitoring of biorecognition events in a label-free mode of operation [19,[26][27][28]. This method is based on the application of small amplitude alternating voltage (perturbation) to the interfacial layer on the electrode, measuring the double layer capacitance (C DL ) and charge transfer resistance (R CT ), respectively [29][30][31]. Moreover, recently we developed reliable EIS assay for detection of glycoproteins directly in human serum with low level of non-specific binding [28].…”