Comparing growth development of Myriophyllum spp. in laboratory and field experiments for ecotoxicological testing

Knauer, Katja; Mohr, Silvia; Feiler, Ute

doi:10.1007/s11356-008-0008-1

Cited by 31 publications

(22 citation statements)

References 31 publications

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“…The apparently higher oxygen saturation (e.g., at the 10 mg/L herbicide concentration) could be caused by algae being resistant to sulfonylurea herbicides [36]. Although macrophyte growth is highly dependent on such environmental parameters as nutrient availability and light intensity, and thus varies among test systems and study approaches, the growth of the total shoot length observed in the present study was in the range of laboratory studies and pond microcosm and mesocosm experiments reviewed by Knauer et al [37].…”

Section: Macrophyte Response To Iofensulfuron-sodiummentioning

confidence: 49%

Response and recovery of the macrophytes Elodea canadensis and Myriophyllum spicatum following a pulse exposure to the herbicide iofensulfuron‐sodium in outdoor stream mesocosms

Wieczorek

Bakanov

Lagadic

et al. 2016

Enviro Toxic and Chemistry

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Interest in stream mesocosms has recently revived for higher tier aquatic macrophyte risk assessment of plant protection products mainly because 1) the highest predicted environmental concentrations for the assessment of effects are frequently derived from stream scenarios, and 2) they allow an effect assessment using stream-typical pulse exposures. Therefore, the present stream mesocosm study used an herbicide pulse exposure and evaluated the responses of Elodea canadensis and Myriophyllum spicatum. Macrophytes were exposed for 24 h to 1 μg/L, 3 μg/L, 10 μg/L, and 30 μg/L of the herbicide iofensulfuron-sodium with a subsequent recovery period of 42 d. Biological endpoints were growth rates of the main, side, and total shoot length, the shoot number, the maximum root length, and the dry weight. The total shoot length was identified as the most sensitive endpoint; the growth rate of the total shoot length was inhibited by up to 66% and 45% in M. spicatum and E. canadensis, respectively. The lowest no observed effect concentrations (NOECs) were observed at day 7 and/or day 14 after herbicide treatment and were 1 μg/L for M. spicatum and 3 μg/L for E. canadensis. The no-observed-ecologically-adverse-effect concentrations (NOEAECs) were 10 μg/L and 30 μg/L for M. spicatum and E. canadensis, respectively. Such or similar mesocosm designs are useful to simulate typical stream exposures and estimate herbicide effects on aquatic macrophytes in stream systems. Environ Toxicol Chem 2017;36:1090-1100. © 2016 SETAC.

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Section: Macrophyte Response To Iofensulfuron-sodiummentioning

confidence: 49%

Response and recovery of the macrophytes Elodea canadensis and Myriophyllum spicatum following a pulse exposure to the herbicide iofensulfuron‐sodium in outdoor stream mesocosms

Wieczorek

Bakanov

Lagadic

et al. 2016

Enviro Toxic and Chemistry

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“…This test was able to indicate phytotoxic effects arising from the investigated sediments . The new Myriophyllum test has already been used in the sediment risk assessment of Lake Skadar , and for comparative studies of simple laboratory bioassays to complex outdoor mesocosm studies with M. aquaticum , M. spicatum , and M. verticillatum . Moreover, it was applied as part of a sediment contact test battery to investigate various unpolluted and polluted sediments as a tool to assess freshwater sediment .…”

Section: Introductionmentioning

confidence: 99%

Inter‐laboratory trial of a standardized sediment contact test with the aquatic plant Myriophyllum aquaticum (ISO 16191)

Feiler

Ratte²,

Arts

et al. 2014

Enviro Toxic and Chemistry

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A whole-sediment toxicity test with Myriophyllum aquaticum has been developed by the German Federal Institute of Hydrology and standardized within the International Organization for Standardization (ISO; ISO 16191). An international ring-test was performed to evaluate the precision of the test method. Four sediments (artificial, natural) were tested. Test duration was 10 d, and test endpoint was inhibition of growth rate (r) based on fresh weight data. Eighteen of 21 laboratories met the validity criterion of r ≥ 0.09 d(-1) in the control. Results from 4 tests that did not conform to test-performance criteria were excluded from statistical evaluation. The inter-laboratory variability of growth rates (20.6%-25.0%) and inhibition (26.6%-39.9%) was comparable with the variability of other standardized bioassays. The mean test-internal variability of the controls was low (7% [control], 9.7% [solvent control]), yielding a high discriminatory power of the given test design (median minimum detectable differences [MDD] 13% to 15%). To ensure these MDDs, an additional validity criterion of CV ≤ 15% of the growth rate in the controls was recommended. As a positive control, 90 mg 3,5-dichlorophenol/kg sediment dry mass was tested. The range of the expected growth inhibition was proposed to be 35 ± 15%. The ring test results demonstrated the reliability of the ISO 16191 toxicity test and its suitability as a tool to assess the toxicity of sediment and dredged material.

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“…Both Myriophyllum species are dicotyledonous, rooted macrophytes, which will be a meaningful compliment to the monocotyledonous, free-floating macrophyte Lemna. These species show high growth rates and a high potential of vegetative reproduction via scions and are relatively easy to handle in the laboratory [13,14], which are important qualifications for macrophyte biotests.…”

mentioning

confidence: 99%

Impact of heterophylly on the sensitivity of Myriophyllum aquaticum biotests

Ebke¹,

Felten

Dören³

2013

Environ Sci Eur

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Background: Parrot feather (Myriophyllum aquaticum) is an amphibious macrophyte with an inducible heterophylly. M. aquaticum plants adapted to the emersed state have leaves with a distinct cuticle and water repellent properties. In contrast M. aquaticum plants adapted to the submerged state have leaves typical for submerged hydrophytes with a strongly reduced cuticle. The aim of the study was to evaluate if this heterophylly of M. aquaticum affects the results of macrophyte biotests. Therefore, the two model substances atrazine and 2,4-dichlorophenoxyacetic acid (2,4-D) were tested each with three M. aquaticum biotests, the only modified parameter being the adaptation time to submergence (0, 7, 28 days). Results: Root length was the most sensitive growth endpoint regarding the test substances atrazine and 2,4-D. Biotests with plants adapted to the submerged state show three times more sensitive results (EC50 7d 142.2 μg/L, EC50 28d 154.5 μg/L) than biotests with plants without an adaptation phase (EC50 0d 458.8 μg/L) in case of atrazine and five times more sensitive results (EC50 28d 46.9 μg/L, EC50 0d 246.3 μg/L) in case of 2,4-D. Apart from the differences in sensitivity, the differently adapted M. aquaticum plants show a completely different growth behavior. The growth rates based on shoot length were nearly ten times higher in the biotests with not adapted M. aquaticum plants than in the biotests with plants 28 days adapted to submergence. Additional measurements of the quantum yield of PSII could demonstrate that rapid growth in length is not based on photosynthetic carbon assimilation. Conclusions: The heterophylly of M. aquaticum affects significantly the sensitivity of aquatic macrophyte biotests and should be taken into account in the development of a standardized test design. The 2,4-D results show the importance of an additional macrophyte biotest to the Lemna test, where the no observed effect concentration (NOEC) value is more than 30 times lower (7 μg/L) than the NOEC value of Lemna in the literature (270 μg/L). Furthermore, the growth rate endpoint in macrophyte biotests should not be misinterpreted. Rapid shoot elongation of amphibious macrophytes, which become submerged, is mainly caused by ethylene-triggered endogenous processes that are not connected to photosynthetic carbon assimilation and appear to be part of a stress reaction to avoid adverse environmental conditions.

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Comparing growth development of Myriophyllum spp. in laboratory and field experiments for ecotoxicological testing

Cited by 31 publications

References 31 publications

Response and recovery of the macrophytes Elodea canadensis and Myriophyllum spicatum following a pulse exposure to the herbicide iofensulfuron‐sodium in outdoor stream mesocosms

Response and recovery of the macrophytes Elodea canadensis and Myriophyllum spicatum following a pulse exposure to the herbicide iofensulfuron‐sodium in outdoor stream mesocosms

Inter‐laboratory trial of a standardized sediment contact test with the aquatic plant Myriophyllum aquaticum (ISO 16191)

Impact of heterophylly on the sensitivity of Myriophyllum aquaticum biotests

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