Comparing genetic connectivity among Dungeness crab (Cancer magister) inhabiting Puget Sound and coastal Washington

Jackson, Tyler; O’Malley, Kathleen G.

doi:10.1007/s00227-017-3152-7

Cited by 11 publications

(7 citation statements)

References 56 publications

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“…Although Cape Flattery and Puget Sound itself have both been classified as biogeographic barriers due to a bifurcation in ocean currents (Valentine, 1966; Kelly and Palumbi, 2010), there are surprisingly few studies evaluating the genetic structure of species found both within Puget Sound and on the outer coast of Washington. Those that do focus on species with much longer dispersal times than O. lurida (Buonaccorsi et al, 2002; Cunningham et al, 2009; Iwamoto et al, 2015; Siegle et al, 2013; Jackson and O’Malley, 2017). To my knowledge, this is the first study in a marine mollusc to evaluate and identify significant population differentiation among Puget Sound populations and the outer coast.…”

Section: Discussionmentioning

confidence: 99%

Population structure, genetic connectivity, and adaptation in the Olympia oyster (Ostrea lurida) along the west coast of North America

Silliman

2018

Preprint

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6Effective management of threatened and exploited species requires an understanding of both the genetic connectivity among populations and local adaptation. The Olympia oyster (Ostrea lurida), patchily distributed from Baja California to the central coast of Canada, has a long history of population declines due to anthropogenic stressors. For such coastal marine species, population structure could follow a continuous isolation-by-distance model, contain regional blocks of genetic similarity separated by barriers to gene flow, or be consistent with a null model of no population structure. To distinguish between these hypotheses in O. lurida, 13,444 single-nucleotide polymorphisms (SNPs) were used to characterize rangewide population structure, genetic connectivity, and adaptive divergence. Samples were collected across the species range on the west coast of North America, from southern California to Vancouver Island. A conservative approach for detecting putative loci under selection identified 288 SNPs across 129 GBS loci, which were functionally annotated and analyzed separately from the remaining neutral loci. While strong population structure was observed on a regional scale in both neutral and outlier markers, neutral markers had greater power to detect fine-scale structure. Geographic regions of reduced gene flow aligned with known marine biogeographic barriers, such as Cape Mendocino, Monterey Bay, and the currents around Cape Flattery. The outlier loci identified as under putative selection included genes involved in developmental regulation, sensory information processing, energy metabolism, immune response, and muscle contraction. These loci are excellent candidates for future research and may provide targets for genetic monitoring programs. Beyond specific applications for restoration and management of the Olympia oyster, this study lends to the growing body of evidence for both population structure and adaptive differentiation across a range of marine species exhibiting the potential for panmixia. Computational notebooks are available to facilitate reproducibility and future open-sourced research on the population structure of O. lurida. 7 8

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Section: Discussionmentioning

confidence: 99%

Population structure, genetic connectivity, and adaptation in the Olympia oyster (Ostrea lurida) along the west coast of North America

Silliman

2018

Preprint

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“…However, cockles within Hood Canal showed slight evidence of divergence from other sub‐populations. Similarly, differentiation of Hood Canal populations from those in other Puget Sound basins has been observed among Dungeness crab (Jackson & O’Malley, 2017 ) and yelloweye rockfish (Andrews et al, 2018 ). Among all Puget Sound sub‐basins, water in Hood Canal is retained for the longest duration (Ebbesmeyer, 1984 ), making larval retention perhaps the most likely explanation for divergence of Hood Canal populations.…”

Section: Discussionmentioning

confidence: 73%

Population genomics of the basket cockle Clinocardium nuttallii in the southern Salish Sea: Assessing genetic risks of stock enhancement for a culturally important marine bivalve

Dimond

Crim

Unsell³

et al. 2022

Evolutionary Applications

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Coastal Indigenous communities that rely on subsistence harvests are uniquely vulnerable to declines in nearshore species. The basket cockle Clinocardium nuttallii is among the favored foods of Indigenous people along the northwest Pacific coast of North America, yet localized declines in their abundance have led to interest in stock enhancement efforts. We used a population genomics approach to examine potential risks associated with stock enhancement of C. nuttallii in the southern Salish Sea, a large inland estuary that includes Puget Sound. More than 8000 single nucleotide polymorphisms across 349 individuals at 12 locations were assembled de novo using restriction site‐associated DNA sequencing. Results indicated that C. nuttallii within the southern Salish Sea were distinct from those along the outer Pacific coast (FST = 0.021–0.025). Within the southern Salish Sea, C. nuttallii populations appear to be well‐connected despite numerous potential impediments to gene flow; Hood Canal, which experiences the lowest flushing rates of all Puget Sound sub‐basins, was a minor exception to this strong connectivity. We found evidence of isolation by distance within the southern Salish Sea, but the slope of this relationship was shallow, and FST values were low (FST = 0.001–0.004). Meanwhile, outlier analyses did not support the hypothesis that southern Salish Sea sub‐populations are locally adapted. Estimates of effective population size had no upper bound, suggesting potentially very high adaptive capacity in C. nuttallii, but also making it difficult to assess potential reductions in effective population size resulting from stock enhancement. We present several strategies to augment cockle populations for subsistence harvest that would limit risk to the genetic diversity of wild cockle populations.

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“…While we attempted to control for variation between individuals by collecting animals from the same location within a 2-month period, we were unable to control for prior environmental exposure or genetic background of the wild-caught animals we used. Where prior studies have found high genetic variation among individuals within one sampling site 47,48 , we suspect that both genetics and prior environmental exposure likely contributed to the high variation in metabolite abundances among individuals within treatment groups, which may have obscured the different treatment effects. Within-sample variation could be reduced by sampling from a specific tissue rather than using whole animals, however this may be challenging in early stage juvenile Dungeness crabs given their small size.…”

Section: Discussionmentioning

confidence: 91%

Uncovering mechanisms of global ocean change effects on the Dungeness crab (Cancer magister) through metabolomics analysis

Trigg

McElhany

Maher

et al. 2019

Preprint

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The Dungeness crab is an economically and ecologically important species distributed along the North American Pacific coast. To predict how Dungeness crab may physiologically respond to future global ocean change on a molecular level, we performed untargeted metabolomic approaches on individual Dungeness crab juveniles reared in treatments that mimicked current and projected future pH and dissolved oxygen conditions. We found 94 metabolites and 127 lipids responded in a condition-specific manner, with a greater number of known compounds more strongly responding to low oxygen than low pH exposure. Pathway analysis of these compounds revealed that juveniles may respond to low oxygen through evolutionarily conserved processes including downregulating glutathione biosynthesis and upregulating glycogen storage, and may respond to low pH by increasing ATP production. Most interestingly, we found that the response of juveniles to combined low pH and low oxygen exposure was most similar to the low oxygen exposure response, indicating low oxygen may drive the physiology of juvenile crabs more than pH. Our study elucidates metabolic dynamics that expand our overall understanding of how the species might respond to future ocean conditions and provides a comprehensive dataset that could be used in future ocean acidification response studies.

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Comparing genetic connectivity among Dungeness crab (Cancer magister) inhabiting Puget Sound and coastal Washington

Cited by 11 publications

References 56 publications

Population structure, genetic connectivity, and adaptation in the Olympia oyster (Ostrea lurida) along the west coast of North America

Population structure, genetic connectivity, and adaptation in the Olympia oyster (Ostrea lurida) along the west coast of North America

Population genomics of the basket cockle Clinocardium nuttallii in the southern Salish Sea: Assessing genetic risks of stock enhancement for a culturally important marine bivalve

Uncovering mechanisms of global ocean change effects on the Dungeness crab (Cancer magister) through metabolomics analysis

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