Comparing ethylene glycol with glycerol for cryopreservation of canine semen in egg-yolk TRIS extenders

Martins‐Bessa, Ana; Rocha, António; Mayenco-Aguirre, A.

doi:10.1016/j.theriogenology.2006.06.004

Cited by 30 publications

(22 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results clearly demonstrated that the use of rapid cooling at a rate of 2.25 C/min prior to freezing provides the same post-thaw sperm quality, in terms of motility and viability, as the values obtained with the use of a control rate of 0.2 C/min. The results for the sperm quality after thawing, in terms of the motility and viability in all of the experimental groups were relatively high, adequate for artificial insemination and comparable with those reported recently in other studies performed in frozen-thawed canine semen using the Uppsala method [25,26,27]. In addition, we did not observe differences among the groups in the quality of the movement parameters VCL, LIN and ALH, which have been described as indicators of a hyperactivated motility pattern and a pre-capacitation status [28].…”

Section: Discussionsupporting

confidence: 88%

Effects of Rapid Cooling Prior to Freezing on the Quality of Canine Cryopreserved Spermatozoa

et al. 2014

View full text Add to dashboard Cite

The aim of this study was to evaluate the effects of rapid cooling prior to freezing on frozen-thawed canine sperm quality. In experiment 1, centrifuged ejaculates from 6 dogs were pooled, split into 4 aliquots and cryopreserved by the Uppsala procedure using different cooling rates (control, cooling speed 18 C/90 min and average cooling rate 0.2 C/min; rapid, cooling speed 18 C/8 min and average cooling rate 2.25 C/min) in combination with 2 glycerol addition protocols (fractionated or unfractionated). In experiment 2, centrifuged ejaculates from 4 dogs were processed individually using the same cooling rates described in experiment 1 in combination with an unfractionated glycerol addition protocol. Each of the experiments was replicated 5 times. Sperm quality was evaluated after 30 and 150 min of post-thawing incubation at 38 C. Total motility (TM), progressive motility (PM) and quality of movement parameters were assessed using a computerized system, and sperm viability (spermatozoa with intact plasma and acrosome membranes) was assessed using flow cytometry (H-42/PI/FITC-PNA). Values for TM, PM, viable spermatozoa and the quality of movement parameters after thawing were not significantly affected by the cooling rate. The interaction between the cooling rate and the added glycerol protocol was not significant. There were significant differences among the males (P<0.01) in the sperm quality parameters evaluated after thawing. The interaction between the males and the cooling rate was not significant. In conclusion, canine spermatozoa can be cryopreserved using the Uppsala method at an average cooling rate of 2.25 C/min prior to freezing together with addition of fractionated or unfractionated glycerol.

show abstract

Section: Discussionsupporting

confidence: 88%

Effects of Rapid Cooling Prior to Freezing on the Quality of Canine Cryopreserved Spermatozoa

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The declining percentage of most rapid and progressive (subpopulation P 1 ) sperms in the study is seemingly acceptable. Although it has been known that a substantial number (50%) of sperm are damaged during cryopreservation [14]. In this study, the results of an average of 35.17% of the most rapid and progressive sperms subpopulation at post-thaw is higher than that reported by Muiño et al [9], who reported only 25.3% kept in cattle bull semen.…”

Section: Discussioncontrasting

confidence: 60%

Sperm subpopulations, buffalo semen, sperm kinematics, cryopreservation, computer assisted sperm analyzer.

Maylem¹,

Leoveras²,

Venturina³

et al. 2017

JAST-A

View full text Add to dashboard Cite

Abstract:This study aimed to identify and characterize the sperm subpopulations existing in water buffalo semen using a computer assisted sperm analyzer (CASA), as well as assess the effects of cryopreservation on the sperm subpopulation structure and evaluate bull variability. The semen of eight Bulgarian Murrah bulls was collected by four times in an interval of one week each. The semen was cryopreserved following a standard protocol and sperm kinematics was assessed. Clustering methods were applied to individual sperms, forming two significantly different (P < 0.05) subpopulations (P 1 and P 2 ). Subpopulation P 1 represents those spermatozoa that moved most rapidly and progressively (46.29%), and subpopulation P 2 includes spermatozoa with relatively low velocity or poorly motile but with high progressiveness (53.41%). There was a decline on the population of P 1 sperms from fresh (52.52%), pre-freeze (45.73%) to post-thaw (35.17%) stages and significant difference on the sperm kinematics between P 1 and P 2 . A significant decline in the values of distance, velocity and amplitude of lateral head (ALH) parameters were observed at post-thaw stage, while an increase was observed on trajectory and beat cross frequency (BCF) kinematics. Values of sperm kinematics were also significantly different (P < 0.05) among all bulls. The frequency distribution of spermatozoa on both subpopulations P 1 and P 2 was quite similar for all bulls in pre-freeze and post-thaw stages, but with significant (P < 0.05) variability on fresh stage. Bulls with the highest maintained frequency of P 1 sperms are denoted as good freezer bulls. In sum, kinematic characterization of water buffalo sperm and clustering into subpopulation enable to identify bulls that are more resistant to cryopreservation and production of quality semen for genetic propagation.

show abstract

“…Ivanova- Kitcheva et al (1997) added glycerol-containing extender directly to semen and equilibrated semen samples for 180 minutes. Glycerol is the most popular cryoprotectant for the cryopreservation of canine semen, but it exerts a toxic effect on spermatozoa, in particular at higher temperatures (Martins-Bessa et al 2006). In most semen cryopreservation protocols, glycerol is added at 32-37 o C or at room temperature (Peñ a et al 1998, Rota et al 1998, Silva et al 2006).…”

Section: Discussionmentioning

confidence: 99%

The effect of two packaging systems on the post-thaw characteristics of canine sperm

Strzeżek¹,

Polakiewicz²,

Kordan³

2015

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The aim of this study was to compare the effect of different packaging systems on some parameters of cryopreserved canine spermatozoa. The experimental material consisted of the sperm-rich fractions of ejaculates collected from four Beagle dogs. Semen samples for cryopreservation were stored in 0.25 ml plastic straws and two aluminum tubes with a total volume of 5.0 ml. Semen was frozen in static nitrogen vapor for 10 minutes (0.25 ml straws) or 15 and 20 minutes (aluminum tubes). Post-thaw assessments involved the determination of sperm motility parameters using a computer assisted sperm analyzer (CASA), sperm plasma membrane integrity (SPMI), mitochondrial membrane potential (MMP) and acrosome integrity (normal apical ridge, NAR). Regardless of the packaging system applied, no significant differences in total sperm motility (TMOT) or selected kinematic parameters were observed after freezing-thawing. However, spermatozoa frozen in 0.25 mL straws were characterized by improved functionality, in particular mitochondrial function, after thawing. The results indicate that large quantities of canine semen can be frozen in aluminum tubes. Further studies are required, however, to evaluate different freezing and thawing rates of aluminum tubes.

show abstract

Comparing ethylene glycol with glycerol for cryopreservation of canine semen in egg-yolk TRIS extenders

Cited by 30 publications

References 38 publications

Effects of Rapid Cooling Prior to Freezing on the Quality of Canine Cryopreserved Spermatozoa

Effects of Rapid Cooling Prior to Freezing on the Quality of Canine Cryopreserved Spermatozoa

Sperm subpopulations, buffalo semen, sperm kinematics, cryopreservation, computer assisted sperm analyzer.

The effect of two packaging systems on the post-thaw characteristics of canine sperm

Contact Info

Product

Resources

About