Comparing and combining MRE, T1ρ, SWI, IVIM, and DCE‐MRI for the staging of liver fibrosis in rabbits: Assessment of a predictive model based on multiparametric MRI

Liqiu, Zou; Jiang, Jie; Zhang, Hao; Zhong, Weimin; Xiao, Min; Xin, Shunbao; Wang, Yang; Xing, Wei

doi:10.1002/mrm.29126

Cited by 10 publications

(2 citation statements)

References 35 publications

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“…Sedation of rabbits reduced heart rate and breathing frequency while physiological short-term effects should have averaged out during the 8 min MRE examination time. Overall, the coefficient of variation of liver stiffness was 6% which is lower than in other MRE studies in healthy rabbit livers (14%–42%, [ Zou et al, 2016 ; Zou et al, 2020 ; Zou et al, 2022 ]), suggesting a minor effect of physiological fluctuations on the measured mechanical properties.…”

Section: Discussioncontrasting

confidence: 63%

On the relationship between metabolic capacities and in vivo viscoelastic properties of the liver

Shahryari

Keller

Meierhofer

et al. 2023

Front. Bioeng. Biotechnol.

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The liver is the central metabolic organ. It constantly adapts its metabolic capacity to current physiological requirements. However, the relationship between tissue structure and hepatic function is incompletely understood; this results in a lack of diagnostic markers in medical imaging that can provide information about the liver’s metabolic capacity. Therefore, using normal rabbit livers, we combined magnetic resonance elastography (MRE) with proteomics-based kinetic modeling of central liver metabolism to investigate the potential role of MRE for predicting the liver’s metabolic function in vivo. Nineteen New Zealand white rabbits were investigated by multifrequency MRE and positron emission tomography (PET). This yielded maps of shear wave speed (SWS), penetration rate (PR) and standardized uptake value (SUV). Proteomic analysis was performed after the scans. Hepatic metabolic functions were assessed on the basis of the HEPATOKIN1 model in combination with a model of hepatic lipid-droplet metabolism using liquid chromatography–mass spectrometry. Our results showed marked differences between individual livers in both metabolic functions and stiffness properties, though not in SUV. When livers were divided into ‘stiff’ and ‘soft’ subgroups (cutoff SWS = 1.6 m/s), stiff livers showed a lower capacity for triacylglycerol storage, while at the same time showing an increased capacity for gluconeogenesis and cholesterol synthesis. Furthermore, SWS was correlated with gluconeogenesis and PR with urea production and glutamine exchange. In conclusion, our study indicates a close relationship between the viscoelastic properties of the liver and metabolic function. This could be used in future studies to predict non-invasively the functional reserve capacity of the liver in patients.

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Section: Discussioncontrasting

confidence: 63%

On the relationship between metabolic capacities and in vivo viscoelastic properties of the liver

Shahryari

Keller

Meierhofer

et al. 2023

Front. Bioeng. Biotechnol.

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“…19 T1rho mapping reflects the interactions between motion-restricted water molecules and local macromolecular environment; 20 hence, it is utilized to study macromolecular composition and proton due to its sensitivity to low-frequency motional and static processes. 21 Recently, T1rho mapping has been applied to detect liver fibrosis, 22 diffuse myocardial fibrosis, 23 and renal allograft fibrosis, 24,25 suggesting that T1rho mapping is a non-invasive diagnostic tool for fibrosis detection.…”

Section: Impact Statementmentioning

confidence: 99%

Novel small-molecule compound VCP979 attenuates renal fibrosis in male rats with unilateral ureteral obstruction

Min

Chen

Wang

et al. 2023

Exp Biol Med (Maywood)

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Renal fibrosis is a hallmark of chronic kidney disease, while efficient therapy against renal fibrosis is still lacking. In this study, we investigated the role of a novel small-molecule compound VCP979 on renal fibrosis and inflammation in a rat model of unilateral ureteral obstruction (UUO). One week after the UUO surgery, rats were administered VCP979 by gavage for one week, and after treatment, magnetic resonance imaging of T1rho mapping and histopathological analysis were performed to evaluate renal fibrosis in vivo and ex vivo. This study showed that treatment with VCP979 effectively reduced renal fibrosis, extracellular matrix accumulation, and alleviated epithelial–mesenchymal transition in UUO rats, as well as improved renal function. In vivo T1rho mapping displayed increased T1rho values in the UUO rats, which was decreased after VCP979 treatment, and a positive correlation was detected between the T1rho values and the percentage of fibrotic area. Moreover, the administration of VCP979 also ameliorated the inflammatory cytokines expression and the infiltration of macrophages in renal tissues. Mechanistically, VCP979 treatment inhibited the activation of p38 mitogen–activated protein kinase, nuclear factor-kappa B, and transforming growth factor-β1/Smads signaling pathways. These results indicated that VCP979 could be an effective therapeutic agent for alleviating renal fibrosis and inflammation in the rat model of UUO via its antifibrotic and anti-inflammatory effects.

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Evaluation of Aortic Hemodynamics Using Four‐Dimensional Flow of Magnetic Resonance Imaging in Rabbits with Liver Fibrosis

Li,

Lin

et al. 2024

Magnetic Resonance Imaging

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BackgroundLiver fibrosis (LF) precipitates systemic hemodynamic alterations, however, its impact on the aorta remaining undefined.PurposeTo assess aorta hemodynamics changes during LF development in a rabbit model.Study TypeProspective, experimental.Animal ModelThirty 7‐month‐old male rabbits underwent bile duct ligation (BDL) to induce LF.Field Strength/SequenceBiweekly four‐dimensional (4D) flow imaging incorporating a 3D gradient‐echo at 3.0 T scanner for 14 weeks post‐BDL.AssessmentHistopathological exams for 2–5 rabbits were performed at each time point, following each MRI scan. LF was graded using the Metavir scale by a pathologist. 4D flow was analyzed by two radiologists using dedicated postprocessing software. They recorded 4D flow parameters at four aorta sections (aortic sinus, before and after bifurcation of aortic arch, and descending aorta).Statistical TestsThe linear mixed model; Bonferroni correction; Pearson correlation coefficient (r); receiver operating characteristic (ROC) curve; Delong test. The level of significance was set at P < 0.05.ResultsFollowing BDL, the wall shear stress (WSS) (0.23–0.32 Pa), energy loss (EL) (0.27–1.55 mW) of aorta significantly increased at the second week for each plane, peaking at the sixth week (WSS: 0.35–0.49 Pa, EL: 0.57–2.0 mW). So did the relative pressure difference (RPD) (second week: 1.67 ± 1.63 mmHg, sixth week: 2.43 ± 0.63 mmHg) in plane 2. Notably, the RPD in plane 2 at the second week displayed the highest area under ROC curve of 0.998 (specificity: 1, sensitivity: 0.967). LF were found at the second, fourth, and sixth week after BDL, with grade F2, F3, and F4, respectively. The RPD in plane 2 was most strongly correlated with the severity of LF (r = 0.86).Data ConclusionsThe occurrence of LF could increase WSS, EL, and RPD of aorta as early as the second week following BDL.Level of Evidence1Technical EfficacyStage 2

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Comparing and combining MRE, T1ρ, SWI, IVIM, and DCE‐MRI for the staging of liver fibrosis in rabbits: Assessment of a predictive model based on multiparametric MRI

Cited by 10 publications

References 35 publications

On the relationship between metabolic capacities and in vivo viscoelastic properties of the liver

On the relationship between metabolic capacities and in vivo viscoelastic properties of the liver

Novel small-molecule compound VCP979 attenuates renal fibrosis in male rats with unilateral ureteral obstruction

Evaluation of Aortic Hemodynamics Using Four‐Dimensional Flow of Magnetic Resonance Imaging in Rabbits with Liver Fibrosis

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