Comparatively Evaluating the Role of Herb Pairs Containing Angelicae Sinensis Radix in Xin‐Sheng‐Hua Granule by Withdrawal Analysis

Pang, Haijun; Xu, Ding‐Qiao; Tang, Yuping; Zhou, Guisheng; Xu, Hao; Jin, Yi; Zhu, Zhenhua; Shi, Xu-Qin; Yue, Shi‐Jun; Chen, Yanyan; Shi, Haiyang; Duan, Jin‐Ao

doi:10.1155/2020/9456350

Cited by 6 publications

(3 citation statements)

References 30 publications

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“…However, QYD and SXD, the separate components of QYSXD, were not as effective in regulating the above cytokines as the QYSX decoction. Similar studies have also found that the effect of QYSXD is better than that of the separate decoction components [40].…”

Section: Discussionsupporting

confidence: 63%

Quyu Shengxin Decoction Alleviates DSS-Induced Ulcerative Colitis in Mice by Suppressing RIP1/RIP3/NLRP3 Signalling

Wu¹,

Yang

Han

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Purpose. To study the therapeutic effect of Quyu (QY) Shengxin (SX) decoction (QYSXD) in mice with dextran sulfate sodium- (DSS-) induced ulcerative colitis and to investigate the effects of QYSXD on the regulation of the receptor-interacting protein kinase 1 (RIP1)/receptor-interacting protein kinase 3 (RIP3)/nucleotide-binding oligomerization domain-like receptor family pyrin domain protein 3 (NLRP3) signaling pathway. Method. Thirty-six mice were randomly divided into the following 6 groups: the experimental group (QYSX group), the model group (DSS group), the positive control group (5-aminosalicylic acid (5-ASA) group), the control group, the first component group (QY group), and the second component group (SX group). Each group included 6 mice. Ulcerative colitis (UC) was induced in the mice by providing 3.5% DSS in drinking water. The mice were weighed every day to evaluate the disease activity index (DAI). After 7 days, the mice were sacrificed, and colonic tissues were obtained for colon length measurement. The morphological changes in the colon and the pathological scores of the mice in each group were observed by hematoxylin-eosin (HE) staining. The messenger ribonucleic acid (mRNA) and protein expression levels of RIP1, RIP3, dynamin-related protein 1 (Drp1), NLRP3, cysteinyl aspartate specific proteinase-1 (caspase-1), interleukin (IL)-1β, and IL-18 in the colon tissues of the mice in each group were detected and compared by real-time quantitative reverse transcription PCR (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA). The levels of RIP1, RIP3, NLRP3, IL-1β, and IL-8 in the colonic mucosa were detected by ELISA. Western blotting was used to compare the protein expression of Drp1, caspase-1, mitochondrial fission protein 1 (FIS1), and mitophagy-associated protein light chain 3a/b (LC3a/b) among groups. The levels of reactive oxygen species (ROS) in the colonic mucosal cells were compared by immunofluorescence. Results. Compared with those in the DSS group, the mice with DSS-induced colitis in the QYSX group exhibited clearly higher body weights ( P < 0.05 ) and DAI scores ( P < 0.05 ). The colon lengths of the mice in the QYSX group were longer than those in the DSS group ( P < 0.05 ), and the pathological score of the QYSX group was lower than that of the DSS group ( P < 0.05 ). The RIP1, RIP3, Drp1, IL-1β, IL-18, and caspase-1 mRNA levels in the QYSX, 5-ASA, SX, and QY groups were significantly lower than those in the DSS group ( P < 0.05 ), but there were no differences between the QYSX group and the 5-ASA group. The levels of RIP1, RIP3, NLRP3, IL-1β, and IL-18 in the QYSX group were lower than those in the DSS group ( P < 0.01 ). The levels of Drp1, caspase-1, FIS1, and LC3a/b in the QYSX group and the 5-ASA group were lower than those in the DSS group ( P < 0.05 ). The levels of ROS in the colonic mucosal cells in the QYSX, 5-ASA, and QY groups were lower than those in the DSS group ( P < 0.05 ). Conclusion. QYSXD has certain therapeutic effects on DSS-induced colitis in mice and may be as effective as 5-ASA. QY and SX decoctions also have certain effects on colitis; however, these decoctions are not as beneficial as QYSXD. QYSXD may ameliorate colitis by inhibiting the expression of RIP1/RIP3/NLRP3 pathway-related proteins and reversing mitochondrial dysfunction to control inflammation.

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Section: Discussionsupporting

confidence: 63%

Quyu Shengxin Decoction Alleviates DSS-Induced Ulcerative Colitis in Mice by Suppressing RIP1/RIP3/NLRP3 Signalling

Wu¹,

Yang

Han

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…The combination of the two herbs appeared as early as in a classic traditional Chinese medicine “Yimu Wan” created in ancient China ( Jia et al, 2017 ). “Shenghua Decoction” is a classic prescription for the treatment of postpartum hemorrhage, which is recorded in the famous works “ Fu Qingzhu Nv Ke .” On this basis, Xinshenghua granule is derived, which is also a commonly used drug in gynecology, and Pang et al found that danggui-yimucao herb pair (DY) made outstanding contributions to Xinshenghua granule ( Cheng et al, 2020a ; Pang et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Danggui-Yimucao Herb Pair Can Protect Mice From the Immune Imbalance Caused by Medical Abortion and Stabilize the Level of Serum Metabolites

Yue

Bai

et al. 2021

Front. Pharmacol.

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Unintended pregnancy is a situation that every woman may encounter, and medical abortion is the first choice for women, but abortion often brings many sequelae. Angelica sinensis Radix (Danggui) and Leonuri Herba (Yimucao) are widely used in the treatment of gynecological diseases, which can regulate menstrual disorders, amenorrhea, dysmenorrhea, and promote blood circulation and remove blood stasis, but the mechanism for the treatment of abortion is not clear. We determined the ability of Danggui and Yimucao herb pair (DY) to regulate the Th1/Th2 paradigm by detecting the level of progesterone in the serum and the expression of T-bet and GATA-3 in the spleen and uterus. Then, we detected the level of metabolites in the serum and enriched multiple metabolic pathways. The arachidonic acid pathway can directly regulate the differentiation of Th1/Th2 cells. This may be one of the potential mechanisms of DY in the treatment of abortion.

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“…"Shenghua Decoction" is a classic prescription for the treatment of postpartum hemorrhage, which is recorded in the famous works "Fu Qingzhu Nv Ke." On this basis, Xinshenghua granule is derived, which is also a commonly used drug in gynecology, and Pang et al found that danggui-yimucao herb pair (DY) made outstanding contributions to Xinshenghua granule (Cheng et al, 2020a;Pang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Comparatively Evaluating the Role of Herb Pairs Containing Angelicae Sinensis Radix in Xin‐Sheng‐Hua Granule by Withdrawal Analysis

Cited by 6 publications

References 30 publications

Quyu Shengxin Decoction Alleviates DSS-Induced Ulcerative Colitis in Mice by Suppressing RIP1/RIP3/NLRP3 Signalling

Quyu Shengxin Decoction Alleviates DSS-Induced Ulcerative Colitis in Mice by Suppressing RIP1/RIP3/NLRP3 Signalling

Danggui-Yimucao Herb Pair Can Protect Mice From the Immune Imbalance Caused by Medical Abortion and Stabilize the Level of Serum Metabolites

Table2.XLSX

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