Comparative Virulence Assessment of Different Nematophagous Fungi and Chemicals against Northern Root-Knot Nematodes, Meloidogyne hapla, on Carrots

Hussain, Manzoor; Maňasová, Marie; Zouhar, Miloslav; Ryšánek, P.

doi:10.17582/journal.pjz/2020.52.1.199.206

Cited by 7 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…on tomato [15]. Moreover, microbial agents for the control of plant-parasitic nematodes is also a potential method; such as bacteria [16,17], fungi [18,19] and actinomycetes [20], which are nematophagous or antagonistic for root-knot nematodes. Specifically, Arthrobotrys irregularis, Pochonia chlamydosporium, Paecilomyces lilacinus, Myrothecium verrucaria, bacteria Pasteuria usgae, Bacillus firmus, Burkholderia cepacia, Pseudomonas fluorescens, and Streptomyces avermitilis [21,22] have been commercially used in many countries for the control of plant-parasitic nematodes.…”

Section: Introductionmentioning

confidence: 99%

Nematicidal Evaluation and Active Compounds Isolation of Aspergillus japonicus ZW1 against Root-Knot Nematodes Meloidogyne incognita

Wang

et al. 2020

Agronomy

View full text Add to dashboard Cite

The root-knot nematode is one of the most damaging plant-parasitic nematodes worldwide, and the ecofriendly alternative approach of biological control has been used to suppress nematode populations. Here the nematicidal activity of Aspergillus japonicus ZW1 fermentation filtrate against Meloidogyne incognita was evaluated in vitro and in greenhouse, and the effects of A. japonicus ZW1 fermentation filtrate on seed germination and the active compound of A. japonicus ZW1 fermentation filtrate were determined. The 2-week fermentation filtrate (2-WF) of A. japonicus ZW1 exhibited markedly inhibitory effects on egg hatching, and 5% 2-WF showed potential nematicidal activities on second-stage juveniles (J2s); the mortality of J2s was 100% after 24 h exposure. The internal contents of nematodes were degraded and remarkable protruded wrinkles were present on the body surface of J2s. The nematicidal activity of the fermentation was stable after boiling and was not affected by storage time. A germination assay revealed that 2-WF did not have a negative effect on the viability and germination of corn, wheat, rice, cowpeas, cucumbers, soybeans, or tomato seeds. The pot-grown study confirmed that a 20% fermentation broth solution significantly reduced root galls and egg numbers on tomatoes, and decreased galls and eggs by 47.3% and 51.8% respectively, over Czapek medium and water controls. The active compound from the A. japonicus ZW1 fermentation filtrate was isolated and identified as 1,5-Dimethyl Citrate hydrochloride ester on the basis of nuclear magnetic resonance (NMR) and LC-MS (liquid chromatograph-mass spectrometer) techniques. Thus, fermentation of A. japonicus ZW1 could be considered a potential new biological nematicide for the control of M. incognita.

show abstract

Section: Introductionmentioning

confidence: 99%

Nematicidal Evaluation and Active Compounds Isolation of Aspergillus japonicus ZW1 against Root-Knot Nematodes Meloidogyne incognita

Wang

et al. 2020

Agronomy

View full text Add to dashboard Cite

show abstract

“…Our results are also consistent with previous work on the predatory activity of A. oligosporus controlling second instar juveniles of Meloidogyne spp. This fungus specializes in the formation of capture nets and demonstrates the ability to attract nematodes [42][43][44][45] and the production of toxins that paralyze the movement of juveniles 46 .…”

Section: Discussionmentioning

confidence: 99%

Assessing the predatory activity of Arthrobotrys oligosporus strain C-2197 as biocontrol of the root-knot nematode Meloidogyne spp.

Quevedo

Vera-Morales

Espinoza-Lozano

et al. 2021

View full text Add to dashboard Cite

The root-knot nematode, Meloidogyne spp., is an endoparasite that infects plants' root system and causes yield losses in several important crops. Meloidogyne is one of the most devastating pests, so searching for effective biological agents is needed to mitigate its damage. In this study, the predatory activity of Arthrobotrys oligosporus Fresen strain C-2197, obtained from a tropical dry forest of Ecuador, was evaluated as a biocontrol alternative for root-knot caused by Meloidogyne spp. Our results showed that A. oligosporus C-2197 has predatory activity against juvenile nematodes, 72.31%, and 79% efficacy, for in vitro and greenhouse conditions. Besides, the studied strain showed growth-promoting activity, increasing leaf and root area of inoculated plants. Growth promoting activity was also observed in field tests. The present study validates the potential use of A. oligosporus as a biocontrol of Meloidogyne spp. in tomato production systems under greenhouse. It also presents useful information on the use of different cultivation media and substrates for massive A. oligosporus spore concentrates.

show abstract

“…Fungal plant-parasitic nematodes can invade the roots of plants and enter host tissues, thereby limiting the effectiveness of chemical pesticides in controlling nematodes. Moreover, the potential toxicity of chemical pesticides poses a substantial threat to human health and environmental safety ( Hussain et al, 2020 ). Thus, there is an urgent need for an environmentally friendly and effective method to prevent and control nematodes.…”

Section: Introductionmentioning

confidence: 99%

Function discovery of a non-ribosomal peptide synthetase-like encoding gene in the nematode-trapping fungus Arthrobotrys oligospora

Liu

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

In this study, the function of a non-ribosomal peptide synthetase-like (NRPS-like) encoding gene AOL_s00188g306 (g306) was investigated to reveal the association between NRPS and nematocidal activity in the nematode-trapping fungus Arthrobotrys oligospora. Sequence analysis indicated that the encoded product of g306 is an adenylation domain of non-ribosomal peptide synthetases and extended short-chain dehydrogenase/reductase domain-containing proteins, and displays a wide substrate spectrum. The Δg306 mutants were more sensitive to chemical stressors than the wild type. Disruption of g306 impeded the nematocidal efficiency of A. oligospora. Metabolomics analysis showed that secondary metabolite biosynthesis and lipid metabolism were altered in the mutants. The phenotypic changes in the mutants can be attributed to the down-regulation of various metabolites, including fatty acyls, prenol lipids, steroidsand steroid derivative, and amino acid derivatives, identified in the present study. This study investigated the association between the non-ribosomal polypeptide-encoding gene g306 and nematicidal activity in A. oligospora, providing a reference for resolving the predation mechanism of nematode-trapping fungus.

show abstract

Comparative Virulence Assessment of Different Nematophagous Fungi and Chemicals against Northern Root-Knot Nematodes, Meloidogyne hapla, on Carrots

Cited by 7 publications

References 22 publications

Nematicidal Evaluation and Active Compounds Isolation of Aspergillus japonicus ZW1 against Root-Knot Nematodes Meloidogyne incognita

Nematicidal Evaluation and Active Compounds Isolation of Aspergillus japonicus ZW1 against Root-Knot Nematodes Meloidogyne incognita

Assessing the predatory activity of Arthrobotrys oligosporus strain C-2197 as biocontrol of the root-knot nematode Meloidogyne spp.

Function discovery of a non-ribosomal peptide synthetase-like encoding gene in the nematode-trapping fungus Arthrobotrys oligospora

Contact Info

Product

Resources

About