2017
DOI: 10.7717/peerj.3142
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Abstract: BackgroundThe monocled cobra (Naja kaouthia) is a medically important venomous snake in Southeast Asia. Its venom has been shown to vary geographically in relation to venom composition and neurotoxic activity, indicating vast diversity of the toxin genes within the species. To investigate the polygenic trait of the venom and its locale-specific variation, we profiled and compared the venom gland transcriptomes of N. kaouthia from Malaysia (NK-M) and Thailand (NK-T) applying next-generation sequencing (NGS) tec… Show more

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Cited by 57 publications
(45 citation statements)
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References 110 publications
(180 reference statements)
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“…50 amino acids) peptides with four conserved disulfide bonds. Sequences coding for waprins were first identified in snakes [ 51 ], then in the frog Ceratophrys calcarata [ 52 ], and recently in scorpions [ 8 , 9 ]. Nawaprin was isolated from the venom of the cobra Naja nigricollis [ 53 ], confirming that waprins are indeed expressed.…”
Section: Resultsmentioning
confidence: 99%
“…50 amino acids) peptides with four conserved disulfide bonds. Sequences coding for waprins were first identified in snakes [ 51 ], then in the frog Ceratophrys calcarata [ 52 ], and recently in scorpions [ 8 , 9 ]. Nawaprin was isolated from the venom of the cobra Naja nigricollis [ 53 ], confirming that waprins are indeed expressed.…”
Section: Resultsmentioning
confidence: 99%
“…Mass spectra was acquired using mass hunter acquisition software (Agilent, USA) in a MS/MS mode with a MS scan range of 200-3000 m/z and MS/MS scan range of 50-3200 m/z. Data were extracted with MH+ mass range between 50 and 3200 Da and processed with Agilent Spectrum Mill MS Proteomics Workbench software packages version B.04.00 against merged database incorporating both non-redundant NCBI databases of Serpentes (taxid: 8570) and in-house transcripts database as previously described [26,27]. Carbamidomethylation was specified as a fixed modification and oxidized methionine as a variable modification.…”
Section: In-solution Tryptic Digestion and Protein Identification By mentioning
confidence: 99%
“…The differences between the two species of Russell’s vipers are, in fact, not limited to their morphology and molecular phylogenies. Differences in the envenoming effects of the Russell’s viper have been reported, attributable to the plasticity of snake venom as an adaptive polygenic trait of venomous snakes 5 , 6 . The observed variations of the envenomation, however, did not conform to the phylogenetics and systematics 7 9 .…”
Section: Introductionmentioning
confidence: 99%