Comparative ultrastructural analysis of the in vitro microspore embryoids and in vivo zygotic embryos of wheat as a basis for understanding of cytophysiological aspects of their development

Seldimirova, O.A.; Круглова, Н.Н.; Titova, G. E.; Batygina, T. B.

doi:10.1134/s1062360417030109

Cited by 13 publications

(8 citation statements)

References 30 publications

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“…The hydrolysis of triglycerides (TAGs) that are stored in lipid bodies generates free fatty acids that are later oxidized to produce acetyl‐CoA. Acetyl‐CoA is metabolized via the glyoxylate cycle, which results in the production of sucrose in the cytosol (Bornman et al ), which can be subsequently stored as starch, an energy source (Seldimirova and Kruglova , Seldimirova et al ). Pinto et al (), on the other hand, highlight the possible influence of the sucrose present in the culture medium on the production of starch in systems in vitro.…”

Section: Discussionmentioning

confidence: 99%

Histology, histochemistry and ultrastructure of pre‐embryogenic cells determined for direct somatic embryogenesis in the palm tree Syagrus oleracea

Silva‐Cardoso

Meira

Gomes³

et al. 2019

Physiologia Plantarum

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Somatic embryogenesis in palm trees is, in general, a slow and highly complex process, with a predominance of the indirect route and, consequently, a lack of knowledge about the direct route. We present new knowledge related to the morphological, histochemical and ultrastructural aspects of the transition from somatic to embryogenic cells and direct formation of somatic embryos from mature zygotic embryos of Syagrus oleracea, a palm tree. The results support the general concept that 2,4‐dichlorophenoxyacetic acid plays a critical role for the formation of somatic embryos of direct and multicellular origin. Seven days in medium with auxin were enough for the identification of embryogenic cells. These cells had a set of characteristics corresponding to totipotent stem cells. At 14 days on induction medium, nodular formations were observed in the distal region of inoculated embryos, which evolved into globular somatic embryos. At 120 days on induction medium, the quality of the somatic embryos was compromised. The dynamics of the mobilization of reserve compounds was also demonstrated, with emphasis on starch and protein as energy sources required for the embryogenic process. This study shows for the first time the anatomical and ultrastructural events involved in direct somatic embryogenesis in a palm tree and incites the scientific community to return to the discussion of classical concepts related to direct somatic embryogenesis, especially regarding the characteristics and location of determined pre‐embryogenic cells.

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Section: Discussionmentioning

confidence: 99%

Histology, histochemistry and ultrastructure of pre‐embryogenic cells determined for direct somatic embryogenesis in the palm tree Syagrus oleracea

Silva‐Cardoso

Meira

Gomes³

et al. 2019

Physiologia Plantarum

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“…В ходе созревания зиготы злаков происходит существенная ее реорганизация: изменяется объем; увеличивается число органоидов; образуются полисомы и мРНК; возрастает количество белка, РНК, ДНК; изменяется метаболизм углеводов, который проявляется в образовании непрерывной клеточной стенки и соответственной активности диктиосом; исчезают плазмодесмы из оболочки, благодаря чему зигота оказывается изолированной от зародышевого мешка и окружающих материнских тканей; создается гетероциклическая система ядра [28].…”

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“…На примере пшеницы выявлено [28], что клетки зародышей злаков в фазе бластомеризации (и далее в начале фазы органогенеза), как и зигота, характеризуются высокой метаболической активностью, необходимой для синтеза конституционных веществ в ходе серии делений, приводящих к увеличению числа клеток. Это подтверждается наличием в клетках зародышей хорошо развитых митохондрий и пластид; в цитоплазме клеток зародышей визуально возрастает количество и длина цистерн ЭПР; агранулярный ЭПР располагается, как правило, между липидными каплями, и, по-видимому, участвует в их синтезе; пролиферация агранулярного ЭПР связана с синтезом материалов, необходимых для построения клеточных стенок.…”

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Periodizations of Cereal Zygotic Embryogenesis in Planta and It’s Use in Biotechnological Research in Vitro

Zinatullina¹

2022

Proceedings of the RAS Ufa Scientific Centre

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In modern biotechnological studies of commercially valuable cereals, promising areas of great interest are the embryo culture and callus culture in vitro, associated with the use of zygotic embryos of different ages that form full-fledged regenerated plants. These biotechnologies are largely based on the use of the morphogenetic potential of embryonic cells, mainly immature ones. At the same time, the progress of such biotechnologies is limited by the lack of a single unified periodization of cereal zygotic embryogenesis in planta. The difficulty of creating a single periodization is due to both the specifics of the course of cereal embryogenesis according to a special Graminad-type and the morphology of the mature embryo, which is characterized by unique organs – the scutellum, coleoptile, epiblast, mesocotylt, coleorhiza. The article provides a brief review of the works devoted to the identification of the structural features of cereal embryos in the dynamics of development in natural conditions from the zygote to the mature structure. Special attention is paid to the comparative analysis of the proposed periodizations of zygotic embryogenesis in planta, especially those used in biotechnological studies in vitro of members of this family. It is emphasized that biotechnological and other experimental studies using cereal embryos should be based on the data of classical descriptive plant embryology.

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“…Donor tillers should be harvested when the developmental stages of the microspores in anthers (uninucleate vacuolated microspores) are at the narrow range, viz, at early, mid, or late uninucleate stages, in order to ensure an effective anther culture technique and induce in vitro wheat anther culture androgenesis. In anther-and isolated microspore culture of wheat, the microspore embryogenic process was induced and tracked to examine the initial cell division and embryo formation of microspores (Indrianto et al, 2001;Datta, 2005;Dwivedi et al, 2015;Seldimirova et al, 2017;Niazian & Shariatpanahi, 2020). Depending on previous publications, most researchers isolated anthers that contain microspores at mid-to-late uninucleate stages (Soriano et al, 2007(Soriano et al, , 2008Broughton, 2008Broughton, , 2011Redha & Suleman, 2011;Rubtsova et al, 2013;Castillo et al, 2015;Weigt et al, 2016Weigt et al, , 2019Weigt et al, , 2020Lazaridou et al, 2016;Broughton et al, 2020;Orlowska et al, 2020).…”

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confidence: 99%

In vitro anther culture as efficiently applied technique for doubled haploid production of wheat (Triticum aestivum L.)

Zuhair¹,

Lantos²,

Pauk³

2021

Ratar i povrt

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In vitro anther culture is the most commonly-used technology for doubled haploid production in wheat (Triticum aestivum L.) and various cereals. The technology application allows the genetic attainment of homozygous pure lines from heterozygous breeding material in a single generation, and has become a rapid alternative to the conventional breeding methods. This technology also contributes to more accurately assess QTL × environmental interactions and has been applied in: genetic researches for marker-trait association study, genomics and as a target for transformation, genetic engineering, gene mapping, and mapping of quantitative trait loci (QTLs). This paper reviews the most important factors that limit the efficiency of winter wheat (Triticum aestivum L.) anther culture, such as genotype dependency and albinism, in addition to other affecting factors including the collection time of tillers and physiological growth environments of plants. Factors that improve the efficiency of wheat anther culture in mitigating both genotypic dependency and albinism have been included in this review, too, for example, genetic improvements by following a strategy to realize this purpose through using responsive breeding material for crossing, application of convenient stress pre-treatments (cold pre-treatments, heat shock treatment, colchicine, hormones, and chemicals) to induce androgenesis, and improvement of the composition of anther culture media and culture conditions. We have also highlighted the methods of chromosome doubling for the haploid wheat produced by anther culture androgenesis, such as spontaneous chromosome doubling or use of colchicine and anti-mitotic chemicals at an early stage of anther culture or as root immersion treatment.

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Comparative ultrastructural analysis of the in vitro microspore embryoids and in vivo zygotic embryos of wheat as a basis for understanding of cytophysiological aspects of their development

Cited by 13 publications

References 30 publications

Histology, histochemistry and ultrastructure of pre‐embryogenic cells determined for direct somatic embryogenesis in the palm tree Syagrus oleracea

Histology, histochemistry and ultrastructure of pre‐embryogenic cells determined for direct somatic embryogenesis in the palm tree Syagrus oleracea

Periodizations of Cereal Zygotic Embryogenesis in Planta and It’s Use in Biotechnological Research in Vitro

In vitro anther culture as efficiently applied technique for doubled haploid production of wheat (Triticum aestivum L.)

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