Comparative transfection studies of human ovarian carcinoma cellsin vitro,ex vivo andin vivo with poly(2-(dimethylamino)ethyl methacrylate)-based polyplexes

Wetering, Petra van de; Schuurmans-Nieuwenbroek, N.M.E.; Hennink, Wim E.; Storm, Gert

doi:10.1002/(sici)1521-2254(199905/06)1:3<156::aid-jgm29>3.0.co;2-o

Cited by 56 publications

(39 citation statements)

References 21 publications

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“…4 Improving the in vivo performance of P[DMAEMA] polyplexes requires modifications that direct the polyplex to the tissue or region in which the target cells reside and optimize intracellular delivery steps that facilitate gene expression. In particular, the need to reduce the interaction with blood components and the inability of condensed DNA to efficiently dissociate from the polycation during cytoplasmic transport have been identified as major limitations of P[DMAEMA] polyplexes for in vivo application.…”

Section: P[dmaema] Polyplexes Have Demonstrated Potential To Transfecmentioning

confidence: 99%

Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA

Moselhy

Vira²,

Liu³

et al. 2009

IJN

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Thermoresponsive cationic nanogel (TCNG) networks based on N-isopropylacrylamide (NIPAM), 2-(dimethylamino)ethyl methacrylate (DMAEMA), and quaternary alkyl ammonium halide salts of DMAEMA (DMAEMAQ) were synthesized by dispersion polymerization technique. The thermoresponsive properties of TCNGs and TCNG-salmon sperm DNA (sasDNA) polyplexes were characterized in aqueous media of various pH and ionic strength. The distinct phase-transition properties of P[NIPAM/DMAEMA]/sasDNA and P[NIPAM/ DMAEMAQ]/sasDNA polyplexes were attributed to the condensing capability of polycations and to differences in the spatial distribution of structural charges in quaternized and nonquaternized networks. The findings demonstrate that stable TCNGs can be prepared with controllable responsive properties determined by the nature of the cationic charge incorporated and may have potential as vehicles for DNA delivery.

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Section: P[dmaema] Polyplexes Have Demonstrated Potential To Transfecmentioning

confidence: 99%

Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA

Moselhy

Vira²,

Liu³

et al. 2009

IJN

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“…Although some previous studies have shown that PAMAM dendrimer could facilitate local gene expression in certain organs, such as eyes, lungs, or tumors, with a certain degree of success (1,29,30),in vivo gene delivery of PAMAM dendrimers is still in its initial stages, and its limited efficacy is always associated with its unacceptable in vivo toxicity (31). Furthermore, the utility of these polymers as the vehicles for the most common intramuscular gene delivery has not been explored.…”

Section: Introductionmentioning

confidence: 99%

PEG-conjugated PAMAM Dendrimers Mediate Efficient Intramuscular Gene Expression

Gao

Tang

et al. 2009

AAPS J

152

103

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Abstract. Generations 5 and 6 (G5 and G6) poly(amidoamine) (PAMAM) dendrimers have been shown to be highly efficient nonviral carriers in in vitro gene delivery. However, their high toxicity and unsatisfied in vivo efficacy limit their applications. In this study, to improve their characteristics as gene delivery carriers, polyethylene glycol (PEG, molecular weight 5,000) was conjugated to G5 and G6 PAMAM dendrimers (PEG-PAMAM) at three different molar ratios of 4%, 8%, and 15% (PEG to surface amine per PAMAM dendrimer molecular). Compared with unconjugated PAMAM dendrimers, PEG conjugation significantly decreased the in vitro and in vivo cytotoxicities and hemolysis of G5 and G6 dendrimers, especially at higher PEG molar ratios. Among all of the PEG-PAMAM dendrimers, 8% PEG-conjugated G5 and G6 dendrimers (G5-8% PEG, G6-8% PEG) resulted in the most efficient muscular gene expression when polyplexes were injected intramuscularly to the quadriceps of neonatal mice. Consistent with the in vivo results, these two 8% PEG-conjugated PAMAM dendrimers could also mediate the highest in vitro transfection in 293A cells. Therefore, G5-8% PEG and G6-8% PEG possess a great potential for gene delivery both in vivo and in vitro.

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“…To address the first challenge, Saravolac et al 5 incorporated amphiphilic polyethylene glycol (PEG) into cationic lipids to shield the charges of lipoplex and to provide a means of steric protection. Others have tried various types of polymers, such poly(L-lysine), [6][7] poly(ethyleneimine), [8][9] poly(methacrylate) 10 and polyamidoamine dendrimers. 11,12 To cope with this problem, we have recently described methods for the polymerization of a novel cationic acrylamide lipid and reconstitution of the resultant poly(cationic lipid) (PCL) to yield stable cationic vesicles.…”

Section: Introductionmentioning

confidence: 99%

Poly(cationic lipid)-mediated in vivo gene delivery to mouse liver

et al. 2003

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We have previously demonstrated that liposomes generated from poly(cationic lipid) (PCL) and cholesterol (Chol) have low cytotoxicity, are serum resistant, and display a transfection efficiency in vitro similar to commercially available cationic liposomes. Our in vivo experiments demonstrated that PCL-Chol liposomes bound much less avidly to serum proteins than did liposomes composed of 1,2-bis(dioleoyloxy)-3-(trimethylamonio)propane (DOTAP)-Chol or DO-TAP-L-a dioleoyl phosphatidylethanolamine (DOPE). Injection of the lipoplexes (PCL-Chol+DNA) through the portal vein after partial hepatectomy (PH) led to much higher reporter gene expression (luciferase) in the liver than did naked DNA injection. Marked green fluorescent protein expression was visualized in almost all hepatocytes in the liver of mice receiving lipoplex injection, even in the absence of PH. Subcutaneous injection of thyroid hormone triiodothyromine (T 3 ) significantly promoted hepatocyte regeneration and markedly enhanced PCL-Chol-mediated gene transfer in mouse liver when the lipoplex was administrated through either portal or tail vein. With T 3 pretreatment, PCL-Chol exerted a better gene transfer efficacy in mouse liver than DOTAP-Chol or DOTAP-DOPE. Two injections of lipoplexes through an indwelling catheter in the portal vein extended the transgene expression at a high level when T 3 injection was repeated. In conclusion, our findings demonstrate that the polymerized cationic liposomes are very stable in the blood and are effective agents for in vivo gene delivery, and that thyroid hormone administration offers a noninvasive approach to enhance liposome-mediated liver gene delivery.

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Comparative transfection studies of human ovarian carcinoma cellsin vitro,ex vivo andin vivo with poly(2-(dimethylamino)ethyl methacrylate)-based polyplexes

Cited by 56 publications

References 21 publications

Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA

Characterization of complexation of poly (N-isopropylacrylamide-co-2-(dimethylamino) ethyl methacrylate) thermoresponsive cationic nanogels with salmon sperm DNA

PEG-conjugated PAMAM Dendrimers Mediate Efficient Intramuscular Gene Expression

Poly(cationic lipid)-mediated in vivo gene delivery to mouse liver

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