Comparative transcriptomics reveals RhoE as a novel regulator of actin dynamics in bone-resorbing osteoclasts

Georgess, Dan; Mazzorana, Marlène; Terrado, José; Delprat, Christine; Chamot, Christophe; Guasch, Rosa M.; Pérez‐Roger, Ignacio; Jurdic, Pierre; Machuca-Gayet, Irma

doi:10.1091/mbc.e13-07-0363

Cited by 41 publications

(37 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…normal situation, the OC migrates at an average instantaneous velocity of 0.8 µm/s and with a track persistence value of 0.45 (0 being a constant rotation of the cell around itself and 1 being a perfectly straight line) 97 (Fig. 3).…”

Section: Podosome Patterning In Osteoclastsmentioning

confidence: 99%

Podosome organization drives osteoclast-mediated bone resorption

Georgess

Machuca-Gayet

Blangy

et al. 2014

Cell Adhesion & Migration

Self Cite

154

166

View full text Add to dashboard Cite

Section: Podosome Patterning In Osteoclastsmentioning

confidence: 99%

Podosome organization drives osteoclast-mediated bone resorption

Georgess

Machuca-Gayet

Blangy

et al. 2014

Cell Adhesion & Migration

Self Cite

154

166

View full text Add to dashboard Cite

“…[53][54][55][56][57][58] were also involved in invadosome formation or function, and they can contribute to control invadosome organization and dynamics; but their requirement is not as universal as Cdc42 one's is. Moreover, Cdc42 is the only RhoGTPase to act as an invadosome inducer.…”

Section: Cdc42 Is a Universal Regulator Of Invadosomesmentioning

confidence: 99%

Cdc42 and Tks5

Martino

Paysan

Gest

et al. 2014

Cell Adhesion & Migration

View full text Add to dashboard Cite

“…For example, the Corning® Osteo Assay Surface has been modified with a synthetic crystalline calcium carbonate coating in order to better resemble the surface of bone for assessing the performance of osteoclasts and osteoblasts 48 . Parallel to the development of xeno-free ECM mimics has been the introduction of serum-free (not necessarily protein-or peptide-free -although these also exist) media systems 49 .…”

Section: Development Of Tissue Culture Materialsmentioning

confidence: 99%

The Importance and Clinical Relevance of Surfaces in Tissue Culture

Hickman

Boocock

Pockley

et al. 2016

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Cell and tissue culture has evolved from the use of simple glassware for the propagation of cells and tissues into a comprehensive platform for interrogating complex biological systems, directing cell fate and deriving products with clinical and therapeutic value.However, despite significant advances, current in vitro culture approaches remain limited in their capacity to model the clinical/biological complexities of disease, in part at least due to the deficiencies of existing culture materials. The challenge is therefore to identify innovative materials-based solutions that have greater control over cells in vitro, while better representing biological systems in vivo. Such platforms would be suitable for biomarker discovery and tissue engineering applications. This review examines the development of tissue culture materials, advances in our understanding of cell-surface interactions and the application of this knowledge towards the development of new approaches for better examining biological events.3 he ability to culture cells and tissues in vitro is a fundamental aspect of modern science. Established early in the twentieth century, notably through the work of Harrison R.G. of John Hopkins University 1 , the ability to culture cells and tissues has markedly improved during the intervening period. The field has progressed from an ability to maintain and culture tissue for extended periods, through the discovery and establishment of immortal cell lines, to today, where tissue engineering is making considerable progress in the production of artificial tissues and organs in vitro [2][3][4] . Key to these successes have been advances in the culture surfaces on which cells and tissues are grown.This review summarizes progress in the development of tissue culture materials, highlights current requirements and existing limitations for in vitro culture, and examines their relevance to clinical questions and our current understanding of tissue culture materials design. Although long-established, current culture materials may not always be appropriate for modelling in vivo conditions, and innovative strategies are therefore required in order to overcome existing limitations. Current Issues with Tissue Culture:Numerous articles have highlighted the drawbacks and limitations of current in vitro culture systems 5,6 . Concerns revolve around deficiencies in the culture systems and the tissue they generate. Although the latter can be linked to the quality of the initial cellular material, contamination and/or poor maintenance of historical cell lines 6,7 , it can also result from deficiencies in the culture systems i.e. not all cell populations are amenable to in vitro culture.Problems are compounded once tissue enters in vitro culture, as derived populations are expected to maintain their in vivo relevance. However, cells naturally adapt to the local environment and T 4 prolonged culture of immortalized cell lines results in a progressive divergence from the parental population 6,8,9 . Although loss or gain of ab...

show abstract

Comparative transcriptomics reveals RhoE as a novel regulator of actin dynamics in bone-resorbing osteoclasts

Cited by 41 publications

References 67 publications

Podosome organization drives osteoclast-mediated bone resorption

Podosome organization drives osteoclast-mediated bone resorption

Cdc42 and Tks5

The Importance and Clinical Relevance of Surfaces in Tissue Culture

Contact Info

Product

Resources

About