Comparative transcriptomics of two pathogenic pinewood nematodes yields insights into parasitic adaptation to life on pine hosts

Xia, Yan; Cheng, Xinyue; Wang, Yunsheng; Luo, Ji; Mao, Zhenchuan; Ferris, V. R.; Xie, Bingyan

doi:10.1016/j.gene.2012.05.041

Cited by 34 publications

(41 citation statements)

References 49 publications

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“…A general comparison of transcriptomic profiles did not reveal notorious differences between these species and even when searching for specific groups of proteins, putatively related to nematodes pathogenicity, only small changes were detected. This was mainly in accordance with previous studies using comparative analysis of B. xylophilus and B. mucronatus transcriptomic data which results indicate that the two species have developed similar molecular mechanisms to adapt to life on pine hosts1314. Even though, the use of this combined database in the secretome’s differential analysis allows a higher number of identified protein comparing to those obtained using an annotated B. xylophilus protein database derived from genome BioProject PRJEA6443719 and thus, constitute an important resource for future genomic and proteomic projects on Bursaphelenchus species.…”

Section: Discussionsupporting

confidence: 92%

“…In the last few years, progress has been made in understanding the nature of proteins used by the PWN to successfully invade and feed on trees. Much of this progress has been supported by large-scale expressed sequence tag (EST) transcriptomic projects undertaken for B. xylophilus 101112 and also using comparative analyses for B. xylophilus and the closest related species, B. mucronatus 1314. Bursaphelenchus mucronatus is distributed throughout the Northern Hemisphere and is a prevalent species in Central and North Europe15.…”

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confidence: 99%

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Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysis

Cardoso

Anjo

Fonseca

et al. 2016

Sci Rep

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The pinewood nematode, Bursaphelenchus xylophilus, recognized as a worldwide major forest pest, is a migratory endoparasitic nematode with capacity to feed on pine tissues and also on fungi colonizing the trees. Bursaphelenchus mucronatus, the closest related species, differs from B. xylophilus on its pathogenicity, making this nematode a good candidate for comparative analyses. Secretome profiles of B. xylophilus and B. mucronatus were obtained and proteomic differences were evaluated by quantitative SWATH-MS. From the 681 proteins initially identified, 422 were quantified and compared between B. xylophilus and B. mucronatus secretomes and from these, 243 proteins were found differentially regulated: 158 and 85 proteins were increased in B. xylophilus and B. mucronatus secretomes, respectively. While increased proteins in B. xylophilus secretome revealed a strong enrichment in proteins with peptidase activity, the increased proteins in B. mucronatus secretome were mainly related to oxidative stress responses. The changes in peptidases were evaluated at the transcription level by RT-qPCR, revealing a correlation between the mRNA levels of four cysteine peptidases with secretion levels. The analysis presented expands our knowledge about molecular basis of B. xylophilus and B. mucronatus hosts interaction and supports the hypothesis of a key role of secreted peptidases in B. xylophilus pathogenicity.

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Section: Discussionsupporting

confidence: 92%

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confidence: 99%

Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysis

Cardoso

Anjo

Fonseca

et al. 2016

Sci Rep

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“…Analysis of xenobiotic metabolisms in PWN from the genome49 and the transcriptome10 published in NCBI showed that pathways of detoxification metabolism in the nematode were obviously different from those in the symbiotic bacteria, and two pathways are complementary. As previously reported, cytochrome P450 enzymes are rich in the nematode and xenobiotics degradation by cytochrome P450 (map00980, map00982) are the main pathways1049. However, a few enzymes were involved in benzoate degradation and its related pathways.…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, PWNs have to cope with a rather toxic condition within the pine trees. Our recent study of the PWN transcriptomes showed that the main metabolic pathway for xenobiotics detoxification in PWN is cytochrome P450 metabolism and PWN does not have a functional pathway that contains all enzymes needed for terpenoids degradation10, suggesting that PWNs may utilize alternative mechanisms for terpenoids degradation.…”

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confidence: 99%

Metagenomic analysis of the pinewood nematode microbiome reveals a symbiotic relationship critical for xenobiotics degradation

Cheng

Tian

Wang

et al. 2013

Sci Rep

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Our recent research revealed that pinewood nematode (PWN) possesses few genes encoding enzymes for degrading α-pinene, which is the main compound in pine resin. In this study, we examined the role of PWN microbiome in xenobiotics detoxification by metagenomic and bacteria culture analyses. Functional annotation of metagenomes illustrated that benzoate degradation and its related metabolisms may provide the main metabolic pathways for xenobiotics detoxification in the microbiome, which is obviously different from that in PWN that uses cytochrome P450 metabolism as the main pathway for detoxification. The metabolic pathway of degrading α-pinene is complete in microbiome, but incomplete in PWN genome. Experimental analysis demonstrated that most of tested cultivable bacteria can not only survive the stress of 0.4% α-pinene, but also utilize α-pinene as carbon source for their growth. Our results indicate that PWN and its microbiome have established a potentially mutualistic symbiotic relationship with complementary pathways in detoxification metabolism.

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“…From there it was introduced into Japan, then spread to neighboring East Asian countries, such as China andKorea in 1982 and, respectively, and it was found in Portugal in 1999 (Mota et al, 1999;Mota and Vieira, 2008) and in Spain in 2008 (Abelleira et al, 2011). PWN is the causal agent of pine wilt disease (PWD) in pine trees (Linit, 1988;Yan et al, 2012). PWD typically occurs in mature pine trees 20 or more years old.…”

Section: Introductionmentioning

confidence: 99%

Expressed sequence tags (EST) analysis of a normalized full-length cDNA library from the pinewood nematode (Bursaphelenchus xylophilus)

Ha¹,

Kang²,

Hye³

et al. 2014

Afr. J. Biotechnol.

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The pinewood nematode (Bursaphelenchus xylophilus) infects pine trees and causes pine wilt disease. To clarify the functions and subcellular localization of B. xylophilus genes/proteins transcribed and predicted from mixed stages (egg, J1, J2, J3 J4 and adult), we prepared a normalized full-length B. xylophilus cDNA library and analyzed expressed sequence tags (ESTs) using the Pendant-Pro sequence analysis suite. Most cDNAs inserted into the library ranged from 0.9 to 1.8 kb (average 1.5 kb). The 1,902 ESTs from B. xylophilus consisted of 286 clusters and 1,273 singletons. EST sizes ranged from 9 to 743 bp with a mean of 336 bp. The predicted protein length from B. xylophilus ESTs revealed that most proteins ranged from 50 to 149 amino acids. Enzyme nomenclature (EC) numbers were classified into 133 (8.5%) of 1,559 contigs using UniProt database hits by the EC numbers method. Transmembrane regions of 1559 clusters were predicted using the TMpred algorithm. The 1,559 contigs with transmembrane regions were annotated; 481 (30.8%) contigs were assigned 'above one' domain and 1,078 (69.1%) were assigned 'none.' Additionally, taxonomy was classified for 672 (43.1%) of 1,559 contigs. Of the 1,559 contigs, 685 (43.9%) were assigned gene ontology terms using the gomerger method of contigs, including singletons. Thirty-one (31) contigs of predicted proteins grouped by BLASTP identity values had significant homology to genes expressed in subcellular structures (for example, mitochondrion, plasma membrane, endoplasmic reticulum, nucleus and golgi). B. xylophilus ESTs provide the foundation for research information on related plant parasite nematodes and contribute to finding an important novel parasite control strategy.

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Comparative transcriptomics of two pathogenic pinewood nematodes yields insights into parasitic adaptation to life on pine hosts

Cited by 34 publications

References 49 publications

Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysis

Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysis

Metagenomic analysis of the pinewood nematode microbiome reveals a symbiotic relationship critical for xenobiotics degradation

Expressed sequence tags (EST) analysis of a normalized full-length cDNA library from the pinewood nematode (Bursaphelenchus xylophilus)

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