Comparative transcriptomics analysis of Streptococcus mutans with disruption of LuxS/AI-2 quorum sensing and recovery of methyl cycle

Yuan, Kai; Hou, Lili; Jin, Qiaoqiao; Niu, Chenguang; Mao, Mengying; Wang, Ruirui; Huang, Zhengwei

doi:10.1016/j.archoralbio.2021.105137

Cited by 15 publications

(10 citation statements)

References 50 publications

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“…At 36 h, the QS of V. parahaemolyticus was OpaR-dependent, consistent with a high-density bacterial environment. At the same time, it interacted with the ABC transport system, similar to the conclusion drawn by Yuan et al that the LuxS/AI-2 QS system mainly affected the transport, conversion and metabolism of ABC transporter proteins and carbohydrates (Yuan et al, 2021). Li et al proposed that transcriptomic profiles of Escherichia coli (E. coli) treated with heavy ion (HI) illustrated that HI might perform membrane damage through regulating material transport, inhibited LuxS/AI-2 system, finally impeded biofilm formation (Li et al, 2021).…”

Section: Discussionsupporting

confidence: 81%

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Comparative Transcriptome Analysis Reveals Regulatory Factors Involved in Vibrio Parahaemolyticus Biofilm Formation

Wang

et al. 2022

Front. Cell. Infect. Microbiol.

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Vibrio parahaemolyticus biofilm poses a serious threat to food safety and human health. However, there is limited knowledge of transcriptional regulatory mechanism during the biofilm formation of this organism. Hence, the RNA sequencing technique was employed to compare the differences in transcriptome profiles between planktonic and biofilm state of V. parahaemolyticus ATCC33847 in this study. Collections of mRNA from planktonic and biofilm cells cultured at 25°C for 36 h were sequenced by studying their biological characteristics. The results showed that there were significant differences in the expression levels of 956 genes in biofilms compared with planktonic cells. These differences suggested that two-component regulatory system (TCS) and quorum sensing (QS) regulated V. parahaemolyticus biofilm formation by affecting important factors such as flagellar motility, Extracellular polymeric substance (EPS) secretion, tripartite ATP-independent (TRAP) transport system and ATP binding cassette (ABC) transport system. The present work in transcriptomics serves as a basis for future studies examining the complex network systems that regulate bacterial biofilm formation.

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Section: Discussionsupporting

confidence: 81%

“…At the same time, it interacted with the ABC transport system, similar to the conclusion drawn by Yuan et al. that the LuxS/AI-2 QS system mainly affected the transport, conversion and metabolism of ABC transporter proteins and carbohydrates ( Yuan et al., 2021 ). Li et al.…”

Section: Discussionsupporting

confidence: 75%

Comparative Transcriptome Analysis Reveals Regulatory Factors Involved in Vibrio Parahaemolyticus Biofilm Formation

Wang

et al. 2022

Front. Cell. Infect. Microbiol.

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“…As previously proven, the biofilm formation in S. mutans is regulated by a QS signaling system that moderates the expression of virulence factors in a cell density-dependent manner [ 2 , 57 – 59 ]. Therefore, inhibition of this system results in the reduction of S. mutans biofilm development and can consequently decrease the cariogenic process.…”

Section: Discussionmentioning

confidence: 99%

Quorum quenching of Streptococcus mutans via the nano-quercetin-based antimicrobial photodynamic therapy as a potential target for cariogenic biofilm

et al. 2022

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Background Quorum sensing (QS) system can regulate the expression of virulence factors and biofilm formation in Streptococcus mutans. Antimicrobial photodynamic therapy (aPDT) inhibits quorum quenching (QQ), and can be used to prevent microbial biofilm. We thereby aimed to evaluate the anti-biofilm potency and anti-metabolic activity of nano-quercetin (N-QCT)-mediated aPDT against S. mutans. Also, in silico evaluation of the inhibitory effect of N-QCT on the competence-stimulating peptide (CSP) of S. mutans was performed to elucidate the impact of aPDT on various QS-regulated genes. Methods Cytotoxicity and intracellular reactive oxygen species (ROS) generation were assessed following synthesis and confirmation of N-QCT. Subsequently, the minimum biofilm inhibitory concentration (MBIC) of N-QCT against S. mutans and anti-biofilm effects of aPDT were assessed using colorimetric assay and plate counting. Molecular modeling and docking analysis were performed to confirm the connection of QCT to CSP. The metabolic activity of S. mutans and the expression level of various genes involved in QS were evaluated by flow cytometry and reverse transcription quantitative real-time PCR, respectively. Results Successful synthesis of non-toxic N-QCT was confirmed through several characterization tests. The MBIC value of N-QCT against S. mutans was 128 μg/mL. Similar to the crystal violet staining, the results log10 CFU/mL showed a significant degradation of preformed biofilms in the group treated with aPDT compared to the control group (P < 0.05). Following aPDT, metabolic activity of S. mutans also decreased by 85.7% (1/2 × MBIC of N-QCT) and 77.3% (1/4 × MBIC of N-QCT), as compared to the control values (P < 0.05). In silico analysis showed that the QCT molecule was located in the site formed by polypeptide helices of CSP. The relative expression levels of the virulence genes were significantly decreased in the presence of N-QCT-mediated aPDT (P < 0.05). Conclusions The combination of N-QCT with blue laser as a QQ-strategy leads to maximum ROS generation, disrupts the microbial biofilm of S. mutans, reduces metabolic activity, and downregulates the expression of genes involved in the QS pathway by targeting genes of the QS signaling system of S. mutans.

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“…SEM was performed according to the protocol described by Yuan et al. (2021) to observe morphologic changes of tested strains.…”

Section: Methodsmentioning

confidence: 99%

“…SEM was performed according to the protocol described by Yuan et al (2021) to observe morphologic changes of tested strains. Overnight cultures of S. mutans were harvested and prefixed with 2% glutaraldehyde and 2% paraformaldehyde at 4°C for 16 h then washed with 0.1 M phosphate buffer (pH 7.4), fixed with 2% osmium tetroxide for 1.5 h, and washed with 0.1 M phosphate buffer (pH 7.4).…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Cnm of Streptococcus mutans is important for cell surface structure and membrane permeability

Naka

Matsuoka

Goto

et al. 2022

Front. Cell. Infect. Microbiol.

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Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is a major pathogen of dental caries. The protein Cnm of S. mutans is involved in collagen binding, but its other biological functions are unknown. In this study, a Cnm-deficient isogenic mutant and a complementation strain were generated from a Cnm-positive S. mutans strain to help determine the properties of Cnm. Initially, comparison of the cell surface structure was performed by electron microscopy, which demonstrated that Cnm appears to be localized on the cell surface and associated with a protruding cell surface structure. Deep RNA sequencing of the strains revealed that the defect in Cnm caused upregulated expression of many genes related to ABC transporters and cell-surface proteins, while a few genes were downregulated. The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides. Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains. Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability.

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Comparative transcriptomics analysis of Streptococcus mutans with disruption of LuxS/AI-2 quorum sensing and recovery of methyl cycle

Cited by 15 publications

References 50 publications

Comparative Transcriptome Analysis Reveals Regulatory Factors Involved in Vibrio Parahaemolyticus Biofilm Formation

Comparative Transcriptome Analysis Reveals Regulatory Factors Involved in Vibrio Parahaemolyticus Biofilm Formation

Quorum quenching of Streptococcus mutans via the nano-quercetin-based antimicrobial photodynamic therapy as a potential target for cariogenic biofilm

Cnm of Streptococcus mutans is important for cell surface structure and membrane permeability

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