Comparative transcriptomic and metabolomic analyses reveal the protective effects of silicon against low phosphorus stress in tomato plants

Zhang, Yi; Chen, Haoting; Liang, Ying; Lu, Tao; Liu, Zhiqian; Jin, Xin; Hou, Leiping; Xu, Jin; Zhao, Hailiang; Shi, Yu; Ahammed, Golam Jalal

doi:10.1016/j.plaphy.2021.05.043

Cited by 25 publications

(11 citation statements)

References 39 publications

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“…Tanzania. A previous study showed that Si has a significant role in the alleviation of abiotic stress in plants ( Zhang et al., 2021 ). In our study, as a result of exogenous SiNPs, wheat seedlings were able to grow more rapidly and had increased chlorophyll contents.…”

Section: Discussionmentioning

confidence: 99%

Effect of nano-silicon on the regulation of ascorbate-glutathione contents, antioxidant defense system and growth of copper stressed wheat (Triticum aestivum L.) seedlings

et al. 2022

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Copper (Cu2+) toxicity can inhibit plant growth and development. It has been shown that silicon (Si) can relieve Cu2+ stress. However, it is unclear how Si-nanoparticles (SiNPs) relieve Cu2+ stress in wheat seedlings. Therefore, the current study was conducted by setting up four treatments: CK, SiNP: (2.5 mM), Cu2+: (500 µM), and SiNP+Cu2+: (2.5 mM SiNP+500 µM Cu2+) to explore whether SiNPs can alleviate Cu2+ toxicity in wheat seedlings. The results showed that Cu2+ stress hampered root and shoot growth and accumulated high Cu2+ concentrations in roots (45.35 mg/kg) and shoots (25.70 mg/kg) of wheat as compared to control treatment. Moreover, Cu2+ treatment inhibited photosynthetic traits and chlorophyll contents as well as disturbed the antioxidant defense system by accumulating malondialdehyde (MDA) and hydrogen peroxidase (H2O2) contents. However, SiNPs treatment increased root length and shoot height by 15.1% and 22%, respectively, under Cu2+ toxicity. Moreover, SiNPs application decreased MDA and H2O2 contents by 31.25% and 19.25%, respectively. SiNPs increased non-enzymatic compounds such as ascorbic acid-glutathione (AsA-GSH) and enhanced superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbic peroxidase (APX) activities by 77.5%, 141.7%, 68%, and 80%, respectively. Furthermore, SiNPs decreased Cu2+ concentrations in shoots by 26.2%, as compared to Cu2+ treatment alone. The results concluded that SiNPs could alleviate Cu2+ stress in wheat seedlings. The present investigation may help to increase wheat production in Cu2+ contaminated soils.

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Section: Discussionmentioning

confidence: 99%

Effect of nano-silicon on the regulation of ascorbate-glutathione contents, antioxidant defense system and growth of copper stressed wheat (Triticum aestivum L.) seedlings

et al. 2022

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“…N transporters (NRT) and P transporters (PHT) play important roles in plant uptake and transport of N and P, respectively ( Zhang et al., 2021c ; Chai et al., 2022 ). NRTs in higher plants include low affinity transporters encoded by some members of the NRT1/PTR (also named as NPF ) family and high affinity transporters encoded by the NRT2 gene family ( Tahir et al., 2021c ; Misawa et al., 2022 ), as well as NRT1.1, a dual-affinity

transporter ( Ye et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of apple dwarfing rootstock root morphogenesis under nitrogen and/or phosphorus deficient conditions

et al. 2023

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Nitrogen (N) and phosphorus (P) are essential phytomacronutrients, and deficiencies in these two elements limit growth and yield in apple (Malus domestica Borkh.). The rootstock plays a key role in the nutrient uptake and environmental adaptation of apple. The objective of this study was to investigate the effects of N and/or P deficiency on hydroponically-grown dwarfing rootstock ‘M9-T337’ seedlings, particularly the roots, by performing an integrated physiological, transcriptomics-, and metabolomics-based analyses. Compared to N and P sufficiency, N and/or P deficiency inhibited aboveground growth, increased the partitioning of total N and total P in roots, enhanced the total number of tips, length, volume, and surface area of roots, and improved the root-to-shoot ratio. P and/or N deficiency inhibited NO3 − influx into roots, and H+ pumps played a important role in the response to P and/or N deficiency. Conjoint analysis of differentially expressed genes and differentially accumulated metabolites in roots revealed that N and/or P deficiency altered the biosynthesis of cell wall components such as cellulose, hemicellulose, lignin, and pectin. The expression of MdEXPA4 and MdEXLB1, two cell wall expansin genes, were shown to be induced by N and/or P deficiency. Overexpression of MdEXPA4 enhanced root development and improved tolerance to N and/or P deficiency in transgenic Arabidopsis thaliana plants. In addition, overexpression of MdEXLB1 in transgenic Solanum lycopersicum seedlings increased the root surface area and promoted acquisition of N and P, thereby facilitating plant growth and adaptation to N and/or P deficiency. Collectively, these results provided a reference for improving root architecture in dwarfing rootstock and furthering our understanding of integration between N and P signaling pathways.

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“…An integrated analysis of the rice transcriptome and metabolome revealed the upregulation of six OsWRKYs in response to low N supply ( Xin et al, 2019 ). The protective effects of silicon against low phosphorus stress in tomato plants might affect the expression of WRKY TFs ( Zhang et al, 2021 ). Notably, recent observations support the involvement of WRKY-mediated crosstalk between abiotic and biotic stress responses ( Wani et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and Expression Profiling of WRKY Family Genes in Sugarcane in Response to Bacterial Pathogen Infection and Nitrogen Implantation Dosage

Javed

Zhou

et al. 2022

Front. Plant Sci.

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WRKY transcription factors (TFs) are essential players in different signaling cascades and regulatory networks involved in defense responses to various stressors. This study systematically analyzed and characterized WRKY family genes in the Saccharum spp. hybrid R570 and their expression in two sugarcane cultivars LCP85-384 (resistant to leaf scald) and ROC20 (susceptible to leaf scald) in response to bacterial pathogen infection and nitrogen implantation dosage. A total of 53 ShWRKY genes with 66 alleles were systematically identified in R570 based on the query sequence SsWRKY in S. spontaneum AP85-441. All ShRWKY alleles were further classified into four groups with 11 (16.7%) genes in group I, 36 (54.5%) genes in group II, 18 (27.3%) genes in group III, and 1 (1.5%) gene in group IV. Among them, 4 and 11 ShWRKY gene pairs displayed tandem and segmental duplication events, respectively. The ShWRKY genes exhibited conserved DNA-binding domains, which were accompanied by variations in introns, exons, and motifs. RT-qPCR analysis of two sugarcane cultivars triggered by Xanthomonas albilineans (Xa) revealed that four genes, ShWRKY13-2/39-1/49-3/125-3, exhibited significant upregulation in leaf scald-resistant LCP85-384. These WRKY genes were downregulated or unchanged in ROC20 at 24–72 h post-inoculation, suggesting that they play an important role in defense responses to Xa infection. Most of the 12 tested ShWRKYs, ShWRKY22-1/49-3/52-1 in particular, functioned as negative regulators in the two cultivars in response to a range of nitrogen (N) implantation doses. A total of 11 ShWRKY proteins were predicted to interact with each other. ShWRKY43 and ShWRKY49-3 are predicted to play core roles in the interaction network, as indicated by their interaction with six other ShWRKY proteins. Our results provide important candidate gene resources for the genetic improvement of sugarcane and lay the foundation for further functional characterization of ShWRKY genes in response to coupling effects of Xa infection and different N levels.

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Comparative transcriptomic and metabolomic analyses reveal the protective effects of silicon against low phosphorus stress in tomato plants

Cited by 25 publications

References 39 publications

Effect of nano-silicon on the regulation of ascorbate-glutathione contents, antioxidant defense system and growth of copper stressed wheat (Triticum aestivum L.) seedlings

Effect of nano-silicon on the regulation of ascorbate-glutathione contents, antioxidant defense system and growth of copper stressed wheat (Triticum aestivum L.) seedlings

Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of apple dwarfing rootstock root morphogenesis under nitrogen and/or phosphorus deficient conditions

Identification and Expression Profiling of WRKY Family Genes in Sugarcane in Response to Bacterial Pathogen Infection and Nitrogen Implantation Dosage

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