Comparative transcriptomic analysis reveals novel insights into the response to Cr(VI) exposure in Cr(VI) tolerant ectomycorrhizal fungi Pisolithus sp. 1 LS-2017

Shi, Liang; Dong, Pengcheng; Song, Wuyu; ChenXi, Li; Lü, Haining; Wen, Zhugui; Wang, Chunchun; Shen, Zhenguo; Chen, Yahua

doi:10.1016/j.ecoenv.2019.109935

Cited by 10 publications

(8 citation statements)

References 72 publications

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“…It suggests that niaD is potentially a novel Cr(VI) reductase in EMF. In our previous study, the relative transcription of niaD significantly increased after Pisolithus sp.1 was treated by Cr(VI), which indicated that niaD was an Cr(VI)-inducible reducing enzyme ( Shi et al, 2020 ).…”

Section: Discussionmentioning

confidence: 87%

“…Based on the results of transcriptome sequencing ( Shi et al, 2020 ; accession number: SRR8837356 - SRR8837367 ), compared with the control group [without Cr(VI)], the differentially expressed genes in Pisolithus sp.1 were only significantly enriched in the nitrogen metabolism pathway but did not significantly enrich in Pisolithus sp.2, and the relative expression levels of the NR gene niaD and NiR gene niiA in Pisolithus sp.1 were higher in the presence of 10 mg/L Cr(VI) treatment. The 5′ race and 3′ race primers of niaD and niiA genes were designed based on the sequencing results of niaD and niiA ( Table 1 and Supplementary Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…In liquid medium, 75% of Cr(VI) can be reduced to Cr(III), and extracellular reduction of Cr(VI) can be accelerated by hydrogen ions (H + ) generated by Pisolithus sp.1 by reducing the pH in the medium within 12 days ( Shi et al, 2018 ). In addition, through the transcriptome sequencing of Pisolithus sp.1 [Cr(VI)-tolerant strain] and Pisolithus sp.2 [Cr(VI)-sensitive strain] before and after Cr(VI) treatment, the results of comparative transcriptome analysis found that compared with the control group [without Cr(VI)], the differentially expressed genes in Pisolithus sp.1 were only significantly enriched in the nitrogen metabolism pathway but did not significantly enrich in Pisolithus sp.2, and the relative expression levels of the genes encoding nitrate reductase (NR) ( niaD ) and nitrite reductase (NiR) ( niiA ) in Pisolithus sp.1 were higher in the presence of 10 mg/L Cr(VI) treatment ( Shi et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…1 . The cloned niaD and niiA were analyzed to predict the amino acid sequences using the DNAMAN software package(version 7.0.2.176, Lynnon BioSoft, Canada).…”

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confidence: 99%

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Cloning of Nitrate Reductase and Nitrite Reductase Genes and Their Functional Analysis in Regulating Cr(VI) Reduction in Ectomycorrhizal Fungus Pisolithus sp.1

et al. 2022

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Assimilatory-type nitrate reductase (NR) and nitrite reductase (NiR) are the key enzymes that involve in nitrate assimilation and nitrogen cycling in microorganisms. NR and NiR with NADH or NADPH and FMN or FAD domains could be coupled to the reduction process of hexavalent chromium [Cr(VI)] in microorganisms. A new assimilatory-type NR gene (named niaD) and a new assimilatory-type NiR gene (named niiA) are cloned, identified, and functionally characterized by 5′ and 3′ RACE, alignment, annotation, phylogenetic tree, and yeast mutant complementation analyses from Pisolithus sp.1, a dominant symbiotic ectomycorrhizal fungi (EMF) that can assist in phytoremediation. Assimilatory-type niaD and niiA were 2,754 bp and 3,468 bp and encode a polypeptide with 917 and 1,155 amino acid residues, respectively. The isoelectric points of NR (Pisolithus sp.1 NR) and NiR (Pisolithus sp.1 NiR) of Pisolithus sp.1 are 6.07 and 6.38, respectively. The calculated molecular mass of Pisolithus sp.1 NR and Pisolithus sp.1 NiR is 102.065 and 126.914 kDa, respectively. Yeast mutant complementation analysis, protein purification, and activities of NR and NiR under Cr treatment suggest that Pisolithus sp.1 NR is a functional NR that mediates Cr(VI) tolerance and reduction. The multiple alignment demonstrates that Pisolithus sp.1 NR is potentially a nicotinamide adenine dinucleotide phosphate-dependent flavin mononucleotide reductase and also Class II chromate reductase. Our results suggest that Pisolithus sp.1 NR plays a key role in Cr(VI) reduction in the EMF Pisolithus sp.1.

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Section: Discussionmentioning

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…1 . The cloned niaD and niiA were analyzed to predict the amino acid sequences using the DNAMAN software package(version 7.0.2.176, Lynnon BioSoft, Canada).…”

mentioning

confidence: 99%

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Cloning of Nitrate Reductase and Nitrite Reductase Genes and Their Functional Analysis in Regulating Cr(VI) Reduction in Ectomycorrhizal Fungus Pisolithus sp.1

et al. 2022

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“…It is well-known fact that ECM fungal symbiosis improves their host plants tolerance against metal stress ( Jourand et al, 2010 , 2014 ; Sun et al, 2021 ) and that ECM symbiosis application in plant regeneration on metal contaminated land has received considerable focus in global research ( Wen et al, 2017 ; Shi et al, 2019 ; Dagher et al, 2020 ). Various reports concerning HM defense mechanisms in ECM fungi ( Kalsotra et al, 2018 ; Khullar and Reddy, 2018 ; Shi et al, 2018 , 2020 ) or plants ( Hasan et al, 2017 ; Li et al, 2019 ; Schat et al, 2020 ) are available separately. Thus, understanding how ECM symbiosis affects and regulates the different plant defense mechanisms against metal stress has prime importance.…”

Section: Introductionmentioning

confidence: 99%

Role of Ectomycorrhizal Symbiosis Behind the Host Plants Ameliorated Tolerance Against Heavy Metal Stress

Chot

Reddy

2022

Front. Microbiol.

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Soil heavy metal (HM) pollution, which arises from natural and anthropogenic sources, is a prime threat to the environment due to its accumulative property and non-biodegradability. Ectomycorrhizal (ECM) symbiosis is highly efficient in conferring enhanced metal tolerance to their host plants, enabling their regeneration on metal-contaminated lands for bioremediation programs. Numerous reports are available regarding ECM fungal potential to colonize metal-contaminated lands and various defense mechanisms of ECM fungi and plants against HM stress separately. To utilize ECM–plant symbiosis successfully for bioremediation of metal-contaminated lands, understanding the fundamental regulatory mechanisms through which ECM symbiosis develops an enhanced metal tolerance in their host plants has prime importance. As this field is highly understudied, the present review emphasizes how plant’s various defense systems and their nutrient dynamics with soil are affected by ECM fungal symbiosis under metal stress, ultimately leading to their host plants ameliorated tolerance and growth. Overall, we conclude that ECM symbiosis improves the plant growth and tolerance against metal stress by (i) preventing their roots direct exposure to toxic soil HMs, (ii) improving plant antioxidant activity and intracellular metal sequestration potential, and (iii) altering plant nutrient uptake from the soil in such a way to enhance their tolerance against metal stress. In some cases, ECM symbiosis promotes HM accumulation in metal stressed plants simultaneous to improved growth under the HM dilution effect.

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Comparative transcriptome analysis of ectomycorrhizal fungus Pisolithus albus in response to individual and combined stress of copper and cadmium

Chot,

Medicherla,

Reddy

2023

Environ Sci Pollut Res

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Comparative transcriptomic analysis reveals novel insights into the response to Cr(VI) exposure in Cr(VI) tolerant ectomycorrhizal fungi Pisolithus sp. 1 LS-2017

Cited by 10 publications

References 72 publications

Cloning of Nitrate Reductase and Nitrite Reductase Genes and Their Functional Analysis in Regulating Cr(VI) Reduction in Ectomycorrhizal Fungus Pisolithus sp.1

Cloning of Nitrate Reductase and Nitrite Reductase Genes and Their Functional Analysis in Regulating Cr(VI) Reduction in Ectomycorrhizal Fungus Pisolithus sp.1

Role of Ectomycorrhizal Symbiosis Behind the Host Plants Ameliorated Tolerance Against Heavy Metal Stress

Comparative transcriptome analysis of ectomycorrhizal fungus Pisolithus albus in response to individual and combined stress of copper and cadmium

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