Comparative transcriptomic analysis of gill and gonad from Mytilus under antibiotics treatment followed by different bacteria challenge

He, Zhihai; He, Jianyu; Wang, Jianxin; Zhang, Xiaolin; Fan, Meihua; Buttino, Isabella; Qi, Pengzhi; Yan, Xiaojun; Liao, Zhi

doi:10.1016/j.aquaculture.2021.737457

Cited by 7 publications

(9 citation statements)

References 74 publications

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“…Our analysis showed that the expansion and high diversity of caspases may endow M. coruscus with an enhanced capacity to handle and respond to pathogen and environmental stimuli. M. coruscus is particularly resistant to bacterial infections and shows a remarkable specificity of the immune response toward different bacteria [ 53 ]. Most caspase genes including Mc-caspase-3/6/7 were significantly induced in the gill after M. luteus (Gram-positive strains) challenge but were suppressed after V. parahemolyticus (Gram-negative strains) challenge.…”

Section: Discussionmentioning

confidence: 99%

“…These results suggesting that M. luteus activates gill cell apoptosis, while V. parahemolyticus suppresses gill cell apoptosis. Gram-negative strains are dominant in seawater, and M. coruscus has more sensitivity to Gram-positive bacteria than Gram-negative bacteria [ 53 ]. In C. gigs , caspase-3 is an LPS-specific intracellular immune receptor, and the interaction between caspase-3 and LPS specifically inhibits the apoptosis induced by caspase-3 [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…The transcriptome data of developmental stages and different tissues, [ 57 ], the samples under antibiotics, bacteria [ 53 ], copper [ 55 ], and OA stress [ 56 ] were downloaded from the NCBI database (PRJNA689255, PRJNA578350, PRJNA707226, PRJNA301064, and PRJNA543748) and then re-analyzed (Table S3 ). Raw data were filtered using the Trimmomatic software to generate clean reads [ 60 ].…”

Section: Methodsmentioning

confidence: 99%

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Expansion and diversity of caspases in Mytilus coruscus contribute to larval metamorphosis and environmental adaptation

Cao,

Xu,

Xiong

et al. 2024

BMC Genomics

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Background Apoptosis is involved (directly and indirectly) in several physiological processes including tissue remodeling during the development, the turnover of immune cells, and a defense against harmful stimuli. The disordered apoptotic process participates in the pathogenesis of various diseases, such as neoplasms, and chronic inflammatory or systemic autoimmune diseases, which are associated with its inadequate regulation. Caspases are vital components of the apoptotic pathway that are involved in developmental and immune processes. However, genome-wide identification and functional analysis of caspase have not been conducted in Mytilus coruscus, which is an economically important bivalve. Results Here, 47 caspase genes were identified from the genomes of M. coruscus, and the expansion of caspase-2/9 and caspase-3/6/7 genes were observed. Tandem duplication acts as an essential driver of gene expansion. The expanded caspase genes were highly diverse in terms of sequence, domain structure, and spatiotemporal expression profiles, suggesting their functional differentiation. The high expression of the expanded caspase genes at the pediveliger larvae stage and the result of apoptosis location in the velum suggest that the apoptosis mediated by them plays a critical role in the metamorphosis of M. coruscus larvae. In gill, caspase genes respond differently to the challenge of different strains, and most caspase-2/9 and caspase-3/6/7 genes were induced by copper stress, whereas caspase-8/10 genes were suppressed. Additionally, most caspase genes were upregulated in the mantle under ocean acidification which could weaken the biomineralization capacity of the mantle tissue. Conclusions These results provide a comprehensive overview of the evolution and function of the caspase family and enhanced the understanding of the biological function of caspases in M. coruscus larval development and response to biotic and abiotic challenges.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Expansion and diversity of caspases in Mytilus coruscus contribute to larval metamorphosis and environmental adaptation

Cao,

Xu,

Xiong

et al. 2024

BMC Genomics

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“…Total RNA was extracted according to the methods used in our previous study (He et al, 2022) from the mantle tissues of the four mussel groups (CN, CA, DN, and DA). In total, there were twelve samples, including three biological replicates for each group, and each sample was collected from six individuals.…”

Section: Rna Extraction and Sequencingmentioning

confidence: 99%

Transcriptomic response of Mytilus coruscus mantle to acute sea water acidification and shell damage

Liao,

Liu,

Wang

et al. 2023

Front. Physiol.

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Mytilus coruscus is an economically important marine calcifier living in the Yangtze River estuary sea area, where seasonal fluctuations in natural pH occur owing to freshwater input, resulting in a rapid reduction in seawater pH. In addition, Mytilus constantly suffers from shell fracture or injury in the natural environment, and the shell repair mechanisms in mussels have evolved to counteract shell injury. Therefore, we utilized shell-complete and shell-damaged Mytilus coruscus in this study and performed transcriptomic analysis of the mantle to investigate whether the expression of mantle-specific genes can be induced by acute seawater acidification and how the mantle responds to acute acidification during the shell repair process. We found that acute acidification induced more differentially expressed genes than shell damage in the mantle, and the biomineralization-related Gene Ontology terms and KEGG pathways were significantly enriched by these DEGs. Most DEGs were upregulated in enriched pathways, indicating the activation of biomineralization-related processes in the mussel mantle under acute acidification. The expression levels of some shell matrix proteins and antimicrobial peptides increased under acute acidification and/or shell damage, suggesting the molecular modulation of the mantle for the preparation and activation of the shell repairing and anti-infection under adverse environmental conditions. In addition, morphological and microstructural analyses were performed for the mantle edge and shell cross-section, and changes in the mantle secretory capacity and shell inner film system induced by the two stressors were observed. Our findings highlight the adaptation of M. coruscus in estuarine areas with dramatic fluctuations in pH and may prove instrumental in its ability to survive ocean acidification.

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“…The total volume of qRT-PCR is 20 µL, which contains 10 µL 2 × SYBR premix Ex Taq (TaKaRa, Dalian, China), 0.40 µmol/L and 0.1 ± 0.02 µg cDNA template for each primer. The three stage qRT-PCR response was conducted as follows: pre-denaturation at 95°C for 30 seconds, followed by denaturation at 95°C for 5 seconds, annealing at 58-60°C for 20 seconds and extension at 72°C for 20 seconds for 40 cycles, and the transcript levels of the genes were determined by the 2 -DD Ct method and standardized with the reference gene (b-actin) (He et al, 2021). Three samples were tested for each group, with three technical replicates of each test.…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Response of the metabolic and autophagy pathways in Mytilus under starvation

et al. 2022

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Being an industrially valuable species of bivalve, Mytilus is widespread in the area around the eastern coast of China. Starvation is an environmental stress that mussels often encounter in their growth. However, few report carried out on the physiological and biochemical response and molecular regulation and adaptation mechanism of mussels under starvation. To illustrate the molecular mechanism of hosts of Mytilus to starvation, the gill transcriptome was used for analysis before and after 9 days of starvation in Mytilus using the Illumina/HiSeq-2000 deep sequencing platform, accompanied by the study of differentially expressed genes (DEGs). In total, 42.137 GB of clean data were obtained from six sample libraries, the average amount of clean data of each sample is 7.023 GB, and 58,540 unigenes that average 780 bp in length were assembled. Unigenes were illustrated by commenting them against the NR, Swiss-Prot, Pfam, String, GO and KEGG databases. After 9 days of starvation, 2,188 and 2,672 genes were determined to be significantly up- or down-regulated expression genes, separately. Amongst, 4,860 genes were associated with 260 pathways, contains vital enrichment pathways, such as “Metabolic”, “PI3K-Akt signaling pathway”, “Phagosome”, “Apoptosis” and “Lysosome”. In inclusion, autophagy cells were investigated by an electron microscopy, alongside further observations of the expression of autophagy-related genes in gill tissue before and after starvation. The data indicates that the amount of autophagosomes increased and found that atg2, atg6 and atg13 were significantly up-regulated. These results indicated that Mytilus may use autophagy to cope with their damage after starvation. With our research results, we have contributed to a deeper understanding of the role of the molecular mechanisms of immune defence in Mytilus under environmental stress, which has given insights into mussel breeding and the understanding of the molecular mechanisms of Mytilus immune system.

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Comparative transcriptomic analysis of gill and gonad from Mytilus under antibiotics treatment followed by different bacteria challenge

Cited by 7 publications

References 74 publications

Expansion and diversity of caspases in Mytilus coruscus contribute to larval metamorphosis and environmental adaptation

Expansion and diversity of caspases in Mytilus coruscus contribute to larval metamorphosis and environmental adaptation

Transcriptomic response of Mytilus coruscus mantle to acute sea water acidification and shell damage

Response of the metabolic and autophagy pathways in Mytilus under starvation

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