Comparative transcriptome profiling reveals differentially expressed genes involved in flavonoid biosynthesis between biennial and triennial Sophora flavescens

Lei, Haiying; Niu, Tianzeng; Song, Huifang; Bai, Bianxia; Han, Peng; Wang, Zhijun; Liu, Ake

doi:10.1016/j.indcrop.2020.113217

Cited by 9 publications

(8 citation statements)

References 54 publications

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“…For example, environmental stressors such as drought, salt and low temperature induce endogenous abscisic acid (ABA) accumulation [ 10 – 12 ], and the application of exogenous ABA improves the cold resistance of plants [ 13 – 15 ]. To date, transcriptomic analyses in various plant species have identified several stress-responsive metabolites [ 4 , 16 – 18 ], such as chlorophyll, osmoprotectants and plant endogenous hormones [ 19 – 25 ]. ABA is produced via the carotenoid pathway in plastids.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional and physiological data revealed cold tolerance in a photo-thermo sensitive genic male sterile line Yu17S

et al. 2022

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Background Rice is highly sensitive to chilling stress during the seedling stage. However, the adaptable photo-thermo sensitive genic male sterile (PTGMS) rice line, Yu17S, exhibits tolerance to low temperatures. Currently, the molecular characteristics of Yu17S are unclear. Results To evaluate the molecular mechanisms behind cold responses in rice seedlings, a comparative transcriptome analysis was performed in Yu17S during seedling development under normal temperature and low temperature conditions. In total, 9317 differentially expressed genes were detected. Gene ontology and pathway analyses revealed that these genes were involved mostly in photosynthesis, carotenoid biosynthesis, carbohydrate metabolism and plant hormone signal transduction. An integrated analysis of specific pathways combined with physiological data indicated that rice seedlings improved the performance of photosystem II when exposed to cold conditions. Genes involved in starch degradation and sucrose metabolism were activated in rice plants exposed to cold stress treatments, which was accompanied by the accumulation of soluble sugar, trehalose, raffinose and galactinol. Furthermore, chilling stress induced the expression of phytoene desaturase, 15-cis-ζ-carotene isomerase, ζ-carotene desaturase, carotenoid isomerase and β-carotene hydroxylase; this was coupled with the activation of carotenoid synthase activity and increases in abscisic acid (ABA) levels in rice seedlings. Conclusions Our results suggest that Yu17S exhibited better tolerance to cold stress with the activation of carotenoid synthase activity and increasing of ABA levels, and as well as the expression of photosynthesis-related genes under cold condition in rice seedlings.

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Section: Introductionmentioning

confidence: 99%

Transcriptional and physiological data revealed cold tolerance in a photo-thermo sensitive genic male sterile line Yu17S

et al. 2022

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“…CHS is another key gene in the flavonoid pathway, and it was also induced by B. siamensis in mango fruit in our study. Lei et al [ 46 ] reported that the over-expression of CHS is beneficial to the accumulation of rutin and hesperetin. This suggests that the accumulation of flavonoid compounds can be increased by up-regulating flavonoid biosynthesis genes, thus improving the resistance of fruits against pathogens.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Inducing Effect of Bacillus siamensis on Disease Resistance in Postharvest Mango Fruit

Jiang

Tang

et al. 2022

Foods

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Postharvest anthracnose, caused by the fungus Colletotrichum gloeosporioides, is one of the most important postharvest diseases of mangoes worldwide. Bacillus siamensis (B. siamensis), as a biocontrol bacteria, has significant effects on inhibiting disease and improving the quality of fruits and vegetables. In this study, pre-storage application of B. siamensis significantly induced disease resistance and decreased disease index (DI) of stored mango fruit. To investigate the induction mechanisms of B. siamensis, comparative transcriptome analysis of mango fruit samples during the storage were established. In total, 234,808 unique transcripts were assembled and 56,704 differentially expressed genes (DEGs) were identified by comparative transcriptome analysis. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of DEGs showed that most of the DEGs involved in plant-pathogen interaction, plant hormone signal transduction, and biosynthesis of resistant substances were enriched. Fourteen DEGs related to disease-resistance were validated by qRT-PCR, which well corresponded to the FPKM value obtained from the transcriptome data. These results indicate that B. siamensis treatment may act to induce disease resistance of mango fruit by affecting multiple pathways. These findings not only reveal the transcriptional regulatory mechanisms that govern postharvest disease, but also develop a biological strategy to maintain quality of post-harvest mango fruit.

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“…TFs are proteins that bind DNA in a sequence-specific manner and regulate transcription by acting as recruitment required for RNA polymerase during transcription initiation [ 16 , 48 ]. In plants, TFs can improve disease and stress resistance, mediate growth and development, affect intercellular signaling, regulate the synthesis and accumulation of secondary metabolites, as well as influence plant evolution [ 10 , 16 , 49 , 50 ]. Several studies have demonstrated that the MYB-bHLH-WD40 complex in plants can regulate the biosynthesis of flavonoids, changing the types and contents of metabolites [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the biosynthesis of catechin and gallic acid of our concern is a complex process involving the shikimate pathway, phenylpropanoid biosynthetic pathway, and flavonoid biosynthetic pathway [ 9 ]. Secondary metabolites are usually differentially distributed in diverse tissues of higher plants, which are associated with the tissue-specific expression of biosynthetic enzyme genes and transcript factors (TFs) related to the corresponding biosynthetic pathways [ 10 , 11 , 12 ]. Therefore, elaborating the expression differences of key enzyme genes in different tissues would deepen our understanding of the molecular mechanisms underlying the tissue specificity of the active compounds [ 5 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analyses of Different Rheum officinale Tissues Reveal Differentially Expressed Genes Associated with Anthraquinone, Catechin, and Gallic Acid Biosynthesis

Zhou

Sun

Zhang

et al. 2022

Genes

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Rheum officinale Baill. is an important traditional Chinese medicinal herb, its dried roots and rhizomes being widely utilized to cure diverse diseases. However, previous studies mainly focused on the active compounds and their pharmacological effects, and the molecular mechanism underlying the biosynthesis of these ingredients in R. officinale is still elusive. Here, we performed comparative transcriptome analyses to elucidate the differentially expressed genes (DEGs) in the root, stem, and leaf of R. officinale. A total of 236,031 unigenes with N50 of 769 bp was generated, 136,329 (57.76%) of which were annotated. A total of 5884 DEGs was identified after the comparative analyses of different tissues; 175 and 126 key enzyme genes with tissue-specific expression were found in the anthraquinone, catechin/gallic acid biosynthetic pathway, respectively, and some of these key enzyme genes were verified by qRT-PCR. The phylogeny of the PKS III family in Polygonaceae indicated that probably only PL_741 PKSIII1, PL_11549 PKSIII5, and PL_101745 PKSIII6 encoded PKSIII in the polyketide pathway. These results will shed light on the molecular basis of the tissue-specific accumulation and regulation of secondary metabolites in R. officinale, and lay a foundation for the future genetic diversity, molecular assisted breeding, and germplasm resource improvement of this essential medicinal plant.

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Comparative transcriptome profiling reveals differentially expressed genes involved in flavonoid biosynthesis between biennial and triennial Sophora flavescens

Cited by 9 publications

References 54 publications

Transcriptional and physiological data revealed cold tolerance in a photo-thermo sensitive genic male sterile line Yu17S

Transcriptional and physiological data revealed cold tolerance in a photo-thermo sensitive genic male sterile line Yu17S

Transcriptome Analysis Reveals the Inducing Effect of Bacillus siamensis on Disease Resistance in Postharvest Mango Fruit

Comparative Transcriptome Analyses of Different Rheum officinale Tissues Reveal Differentially Expressed Genes Associated with Anthraquinone, Catechin, and Gallic Acid Biosynthesis

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