Comparative transcriptome profiling of chilling stress responsiveness in grafted watermelon seedlings

Xu, Jinhua; Zhang, Man; Liu, Guang; Yang, Xingping; Hou, Xilin

doi:10.1016/j.plaphy.2016.11.002

Cited by 37 publications

(35 citation statements)

References 53 publications

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“…We speculate that rootstock grafting improved the resistance of cucumber seedling to CA stress due to lower sensitivity. Similar results were also obtained by [17], where weaker response to chilling stress in rootstock grafted than self-grafted watermelon seedlings. In addition, it was found that less of DEGs in RG libraries (42.7%) were down-regulated after exposure to CA treatment compared to NG (46.4%), which indicated that rootstock grafting could keep gene expression stable under adverse condition.…”

Section: Overall Analysis Of Gene Expression Based On Rna-seqsupporting

confidence: 84%

“…Recently, transcriptomic studies have been applied as a powerful tool to better understand the molecular mechanisms involved with resistance to abiotic stress by grafting, such as low temperature [17] and drought stress [18]. A number of differentially expressed genes (DEGs) related to rootstock grafted watermelon regulated chilling tolerance were identified, which are involved in induction of protein processing, plant-pathogen interaction, the spliceosome, and suppression of photosynthesis [17]. It was also discovered based on transcriptomic analysis that grafted tomato could better adapt to drought stress by regulating ABA signal transduction and stomatal aperture [18].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis Reveals the Different Response to Toxic Stress in Rootstock Grafted and Non-Grafted Cucumber Seedlings

Xiao

Xie

et al. 2020

IJMS

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Autotoxicity of root exudates is one of the main reasons for consecutive monoculture problem (CMP) in cucumber under greenhouse cultivation. Rootstock grafting may improve the tolerance of cucumber plants to autotoxic stress. To verify the enhanced tolerance to autotoxic stress and illuminate relevant molecular mechanism, a transcriptomic comparative analysis was performed between rootstock grafted (RG) and non-grafted (NG) cucumber plants by a simulation of exogenous cinnamic acid (CA). The present study confirmed that relatively stable plant growth, biomass accumulation, chlorophyll content, and photosynthesis was observed in RG than NG under CA stress. We identified 3647 and 2691 differentially expressed genes (DEGs) in NG and RG cucumber plants when compared to respective control, and gene expression patterns of RNA-seq was confirmed by qRT-PCR. Functional annotations revealed that DEGs response to CA stress were enriched in pathways of plant hormone signal transduction, MAPK signaling pathway, phenylalanine metabolism, and plant-pathogen interaction. Interestingly, the significantly enriched pathway of photosynthesis-related, carbon and nitrogen metabolism only identified in NG, and most of DEGs were down-regulated. However, most of photosynthesis, Calvin cycle, glycolysis, TCA cycle, and nitrogen metabolism-related DEGs exhibited not or slightly down-regulated in RG. In addition, several stress-related transcription factor families of AP2/ERF, bHLH, bZIP, MYB. and NAC were uniquely triggered in the grafted cucumbers. Overall, the results of this study suggest that rootstock grafting improve the tolerance of cucumber plants to autotoxic stress by mediating down-regulation of photosynthesis, carbon, and nitrogen metabolism-related DEGs and activating the function of stress-related transcription factor. The transcriptome dataset provides an extensive sequence resource for further studies of autotoxic mechanism at molecular level.

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Section: Overall Analysis Of Gene Expression Based On Rna-seqsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Different Response to Toxic Stress in Rootstock Grafted and Non-Grafted Cucumber Seedlings

Xiao

Xie

et al. 2020

IJMS

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“…After 24 days, 19 HSPs were down-regulated in GE, while 19 were up-regulated in JS, which supports the idea that continuous high levels of HSPs may help plants adapt to stress stimuli [45]. DnaJ protein and Chaperonin have also been reported to protect plants against biotic and abiotic stress [46,47]. Herein, chaperone protein Dnaj 10 isoform X2 (XP_008453046.1) was specifically up-regulated in JS after 12 days of cold treatment.…”

Section: Structural Proteinssupporting

confidence: 68%

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

Song

Tang

Cai

et al. 2020

Preprint

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Background: Cantaloupe is susceptible to cold stress when it is stored at low temperatures, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of low temperatures resistance in cantaloupe, a cold-sensitive cultivar, Golden Empress-308 (GE) and a cold-tolerant cultivar, Jia Shi-310 (JS), were selected in parallel for iTRAQ quantitative proteomic analysis. Results: The two kinds of commercial cultivars were exposed to a temperature of 0.5℃ for 0, 12 and 24 days. We found that the cold-sensitive cultivar (GE) suffered more severe damage as the length of the cold treatment increased. Proteomic analysis of both cultivars indicated that the number of differentially expressed proteins (DEPs) changed remarkably during the chilly treatment. JS expressed cold-responsive proteins more rapidly and mobilized more groups of proteins than GE. Furthermore, metabolic analysis revealed that more amino acids were up-regulated in JS during the early phases of low temperatures stress. The DEPs we found were mainly related to carbohydrate and energy metabolism, structural proteins, reactive oxygen species scavenging, amino acids metabolism and signal transduction. The consequences of phenotype assays, metabolic analysis and q-PCR validation confirm the findings of the iTRAQ analysis. Conclusion: We found that the prompt response and mobilization of proteins in JS allowed it to maintain a higher level of cold tolerance than GE, and that the slower cold responses in GE may be a vital reason for the severe chilling injury commonly found in this cultivar. The candidate proteins we identified will form the basis of future studies and may improve our understanding of the mechanisms of cold tolerance in cantaloupe.

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“…Watermelon (Citrullus lanatus L.), a member of the Cucurbitaceae family, is one of the top five mostconsumed fresh fruits globally. In many areas of the world, chilling stress owing to early-spring low temperature is a major environmental stress threatening the watermelon industry 1,2 . Among the several techniques that are commonly used to cope with chilling stress, grafting is one of the most-effective and cost-saving approaches [3][4][5] .…”

Section: Introductionmentioning

confidence: 99%

A universal pipeline for mobile mRNA detection and insights into heterografting advantages under chilling stress

Wang

Xing

et al. 2020

Hortic Res

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Heterografting has long been used to enhance the chilling tolerance of temperature-sensitive crops, including watermelon, whose mechanism is known to involve bidirectional long-distance mRNA movements. Despite several studies reporting on mobile mRNA (mb-mRNA) profiles in plants, accurate identification of mb-mRNAs is challenging owing to an array of technical problems. Here, we developed a bioinformatical pipeline that took most of the known technical concerns into consideration and is considered to be a universal tool for mb-mRNA detection in heterografts. By applying this pipeline to a commercial watermelon-bottle gourd heterografting system, we detected 130 and 1144 mb-mRNAs upwardly and 167 and 1051 mb-mRNAs downwardly transmitted under normal and chilling-stress conditions, respectively. Quantitative real-time PCR indicated a high accuracy rate (88.2%) of mb-mRNA prediction with our pipeline. We further revealed that the mobility of mRNAs was not associated with their abundance. Functional annotation and classification implied that scions may convey the stress signal to the rootstock, subsequently triggering energy metabolism reprogramming and abscisic acid-mediated stress responses by upward movement of effective mRNAs, ultimately leading to enhanced chilling tolerance. This study provides a universal tool for mb-mRNA detection in plant heterografting systems and novel insights into heterografting advantages under chilling stress.

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Comparative transcriptome profiling of chilling stress responsiveness in grafted watermelon seedlings

Cited by 37 publications

References 53 publications

Transcriptome Analysis Reveals the Different Response to Toxic Stress in Rootstock Grafted and Non-Grafted Cucumber Seedlings

Transcriptome Analysis Reveals the Different Response to Toxic Stress in Rootstock Grafted and Non-Grafted Cucumber Seedlings

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

A universal pipeline for mobile mRNA detection and insights into heterografting advantages under chilling stress

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