Comparative Transcriptome-Based Mining of Senescence-Related MADS, NAC, and WRKY Transcription Factors in the Rapid-Senescence Line DLS-91 of Brassica rapa

Yi, So Young; Rameneni, Jana Jeevan; Lee, Myungjin; Song, Seul Gi; Choi, Yuri; Lu, Lu; Lee, Hyeokgeun; Lim, Yong Pyo

doi:10.3390/ijms22116017

Cited by 2 publications

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References 113 publications

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“…MADS-box is a conserved gene family found in plants as well as animals. Many studies have presented evidence for linkages between MADS-box members and senescence in different plant species; for example, Arabidopsis thaliana ( Fang and Fernandez, 2002 ), Solanum lycopersicum ( Xie et al., 2014 ), Medicago truncatula ( De Michele et al., 2009 ), and Brassica rapa ( Yi et al., 2021 ). The overexpression of MADS-box genes usually results in a delayed senescence process in different organs, which demonstrates the role of negative regulators of senescence.…”

Section: Introductionmentioning

confidence: 99%

The MADS-box gene EjAGL15 positively regulates lignin deposition in the flesh of loquat fruit during its storage

2023

Front. Plant Sci.

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IntroductionLignification of fruit flesh is a common physiological disorder that occurs during post-harvest storage, resulting in the deterioration of fruit quality. Lignin deposition in loquat fruit flesh occurs due to chilling injury or senescence, at temperatures around 0°C or 20°C, respectively. Despite extensive research on the molecular mechanisms underlying chilling-induced lignification, the key genes responsible for the lignification process during senescence in loquat fruit remain unknown. MADS-box genes, an evolutionarily conserved transcription factor family, have been suggested to play a role in regulating senescence. However, it is still unclear whether MADS-box genes can regulate the lignin deposition that arises from fruit senescence.MethodsBoth senescence- and chilling-induced flesh lignification were simulated by applying temperature treatments on loquat fruits. The flesh lignin content during the storage was measured. Transcriptomic, quantitative reverse transcription PCR and correlation analysis were employed to identify key MADS-box genes that may be involved in flesh lignification. The Dual-luciferase assay was utilized to identify the potential interactions between MADS-box members and genes in phenylpropanoid pathway. Results and DiscussionThe lignin content of the flesh samples treated at 20°C or 0°C increased during storage, but at different rates. Results from transcriptome analysis, quantitative reverse transcription PCR, and correlation analysis led us to identify a senescence-specific MADS-box gene, EjAGL15, which correlated positively with the variation in lignin content of loquat fruit. Luciferase assay results confirmed that EjAGL15 activated multiple lignin biosynthesis-related genes. Our findings suggest that EjAGL15 functions as a positive regulator of senescence-induced flesh lignification in loquat fruit.

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Section: Introductionmentioning

confidence: 99%

The MADS-box gene EjAGL15 positively regulates lignin deposition in the flesh of loquat fruit during its storage

2023

Front. Plant Sci.

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MADS-Box Subfamily Gene GmAP3 from Glycine max Regulates Early Flowering and Flower Development

Zhang

et al. 2023

IJMS

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AP3 has been studied and is reported to affect structural changes in floral organs in various plants. However, the function of the soybean AP3 genes in flower development is unknown. Here, the full-length cDNA sequence of GmAP3 was obtained by RACE and it was verified that it belongs to the MADS-box subfamily by a bioinformatics analysis. The expression of GmAP3 is closely related to the expression of essential enzyme genes related to flower development. Yeast two-hybrid assays demonstrated that GmAP3 interacts with AP1 to determine the identity of flower organ development. A follow-up analysis showed that overexpression of the GmAP3 gene advanced flowering time and resulted in changes in floral organ morphology. The average flowering time of overexpressed soybean and tobacco plants was 6–8 days earlier than that of wild-type plants, and the average flowering time of gene-edited soybean and tobacco plants was 6–11 days later than that of wild-type plants. In conclusion, GmAP3 may directly or indirectly affect the flower development of soybean. The results of this study lay the foundation for further research on the biological functions of MADS transcriptional factors in soybeans.

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Comparative Transcriptome-Based Mining of Senescence-Related MADS, NAC, and WRKY Transcription Factors in the Rapid-Senescence Line DLS-91 of Brassica rapa

Cited by 2 publications

References 113 publications

The MADS-box gene EjAGL15 positively regulates lignin deposition in the flesh of loquat fruit during its storage

The MADS-box gene EjAGL15 positively regulates lignin deposition in the flesh of loquat fruit during its storage

MADS-Box Subfamily Gene GmAP3 from Glycine max Regulates Early Flowering and Flower Development

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