Comparative Transcriptome and Metabolome Analysis of Resistant and Susceptible Piper Species Upon Infection by the Oomycete Phytophthora Capsici

Abstract

Phytophthora capsici is a destructive oomycete pathogen that causes devastating disease in black pepper, resulting in a significant decline in yield and economic losses. Piper nigrum (black pepper) is documented as susceptible to P. capsici, whereas its close relative Piper flaviflorum is known to be resistant. However, the molecular mechanism underlying the resistance of P. flaviflorum remains obscure. In this study, we conducted a comparative transcriptome and metabolome analysis between P. flaviflorum and P. nigrum upon P. capsici infection and found substantial differences in their gene expression profiles, with altered genes being significantly enriched in terms relating to plant-pathogen interaction, phytohormone signal transduction, and secondary metabolic pathways, including phenylpropanoid biosynthesis. Further metabolome analysis revealed the resistant P. flaviflorum to have a high background endogenous ABA reservoir and time-course-dependent accumulation of ABA and SA upon P. capsici inoculation, while the susceptible P. nigrum had a high background endogenous IAA reservoir and time-course-dependent accumulation of JA-Ile, the active form of JA. Investigation of the phenylpropanoid biosynthesis metabolome further indicated the resistant P. flaviflorum to have more accumulation of lignin precursors than the susceptible P. nigrum, resulting in a higher accumulation after inoculation. This study provides an overall characterization of biologically important pathways underlying the resistance of P. flaviflorum, which theoretically explains the advantage of using this species as rootstock for the management of oomycete pathogen in black pepper production.

Identification of Piper species that are resistant to Phytophthora capsici, Meloidogyne incognita, and waterlogging in Vietnam

“…Furthermore, grafts suffered from lower productivity and low drought tolerance (Nguyen et al, 2019). More recently, P. flaviflorum has been tested as a rootstock to create oomycete‐resistant black pepper grafts (Fan et al, 2022). Graft compatibility between resistant rootstocks and high‐yielding scions will probably determine the success of these programmes.…”

UHPLC-HRMS/MS Chemical Fingerprinting of the Bioactive Partition from Cultivated Piper aduncum L.

“…This GNPS analysis generated a family represented by prenylated derivatives of benzoic acid (Figures 3 and S5 The main fragment registered in the MS 2 spectra of this molecular family indicates the loss of the neutral fragment consisting of a prenyl group with 56 mass units. The class of the prenylated derivatives of benzoic acid is known in the species P. aduncum [13,29], including the annotated chromene 68 [10].…”

Transcriptome analysis of Ganoderma lingzhi (Agaricomycetes) response to Trichoderma hengshanicum infection

“…With the development of second-generation sequencing technology and molecular biology, many researchers have explored the molecular regulation mechanisms of rice bacterial blight (Sana et al, 2010), Phytophthora capsici disease (Fan et al, 2022), Nerium indicum witches' broom disease (Wang et al, 2022), Phytophthora sojae disease (Zhu, 2018), apple alternaria blotch disease (Zhu et al, 2017), Chrysanthemum morifolium black spot (Liu L. N. et al, 2021), and wheat leaf spot (Ye et al, 2019). Bailey et al (2013) found that the pathogen (Lecanicillium fungicola) and the host (Agaricus bisporus) changed the expression of their respective genes during the interaction through transcriptome analysis, which initially revealed the host's defense response mechanism.…”