Comparative transcriptome analysis two genotypes of Acer truncatum Bunge seeds reveals candidate genes that influences seed VLCFAs accumulation

Abstract

The Acer truncatum Bunge is a particular widespread forest tree species in northern China. VLCFAs are important to eukaryotes survival and play diverse roles throughout the development. So far, there are reports that the Acer truncatum seeds fatty acid (FA) rich in VLCFAs, but little is known about the physiological mechanism responsible for the biosynthesis. A total of approximately 37.07 Gbp was generated, it was comprehensive enough to determine the majority of the regulation VLCFAs biosynthesis genes. The 97,053 different unigenes were assembled and identified, and large numbers of EST-SSRs were determined. The expression profiles of crucial genes (KCS, KCR, HCD and ECR) involved in VLCFAs elongation of fatty acids were also studied. To our knowledge, the present study provides the first comprehensive of the transcriptome of Acer truncatum seeds. This transcriptome dataset have been made publicly available NCBI, we believe that it may provide new resource for future high-throughput gene expression of Acer truncatum seeds growth and development and will provide theoretical basic information for improving the yield of VLCFAs, especially nervonic acid.

Genome Survey Sequencing of Acer truncatum Bunge to Identify Genomic Information, Simple Sequence Repeat (SSR) Markers and Complete Chloroplast Genome

“…Among a wide range of plants, the assembled Acer truncatum genomic sequence had the highest number of hits against Citrus sinensis ( Figure S1). This result is consistent with previous transcriptome sequencing findings [29]. The N50 of scaffolds and contigs was calculated by ordering all sequences, before adding the lengths from the longest to shortest until the added length exceeded 50% of the total length of all sequences.…”

“…Using the MISA software (http://pgrc.ipk-gatersleben.de/misa/misa.html), 145,640 scaffolds of Acer truncatum were used for genomic SSR marker detection. The following search parameters were set for identification: Di-, Tri-, Tetra-, Penta-and Hexa-nucleotide motifs with a minimum of 6, 5, 4, 4 and 4 repeats, respectively, as previously described [29,30]. The primers for SSR markers were designed by Premier 5.0 (Premier Biosoft International, Palo Alto, CA, USA).…”

Comparative transcriptomics of an arctic foundation species, tussock cottongrass (Eriophorum vaginatum), during an extreme heat event

“…Because variation in ecotypic responses are measurable through field studies, transcriptomics should provide empirical evidence of the genes that have a potential role in ecotypic variation and adaptation while uncovering cryptic variation [31][32][33] . Genes involved in abiotic stress response and metabolic processes would be expected to show variation in expression associated with E. vaginatum ecotypes that go beyond field measurable responses [27][28][29] .…”

Transcriptome Analysis of Acer truncatum Seeds Reveals Candidate Genes Related to Oil Biosynthesis and Fatty Acid Metabolism

“…In 2011, the seed oil was approved as a New Resource Food by the National Health and Family Planning Commission of the People's Republic of China, with potentially important implications for the fields of food and medicine. Previous studies of A. truncatum have mainly focused on its seed oil (Chen et al, 2017;Liang et al, 2019;Wang et al, 2018) that contains UFAs, including oleic acid (C18:1), linoleic acid (C18:2), a-linolenic acid (C18:3), and nervonic acid (C24:1) (Liu et al, 2003;Wang et al, 2006). In particular, the proportions of C18:1 and C18:2 are higher than those in other edible oils, including peanut (Arachis hypogaea), rapeseed (Brassica napus), and sunflower (Helianthus annuus) oils (Zhao et al, 2006).…”

“…However, such research of A. truncatum is limited. The first comprehensive study characterizing the gene expression in A. truncatum seeds was conducted by Wang et al (2018). They compared the transcriptomes of two genotypes of A. truncatum seeds and identified candidate genes that influenced the verylong-chain fatty acid (VLCFA) accumulation.…”