Comparative transcriptome analysis reveals the immune response of turbot (Scophthalmus maximus) induced by inactivated bivalent vaccine

Zhang, Jian; Zhang, Shuning; Sun, Xiangyi; Xu, Xiudan

doi:10.1016/j.fsi.2022.108461

Cited by 1 publication

(2 citation statements)

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“…To date, transcriptome analysis has been commonly employed to investigate immune responses during various pathogenic infections and vaccination trials in several fish species and even in Nile tilapia [ 28 , 50 , 51 , 52 ]. However, the adjuvant effects of On -CC1 during S. agalactiae vaccination of Nile tilapia are still unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the objective of the present research was to evaluate the effectiveness of the On -CC1 chemokine protein as an adjuvant for enhancing immune defense mechanisms and disease resistance against pathogenic S. agalactiae infection in Nile tilapia. Transcriptome analysis is a powerful tool for describing gene expression responses under various conditions at the molecular level [ 22 , 23 , 24 , 25 ], including vaccination-induced responses in various fish species [ 26 , 27 , 28 ]. Similarly, transcriptome analysis of the head kidney of control and vaccinated fish clearly revealed the upregulation of vital immune-related genes induced by a whole-cell inactivated vaccine (WC) and enhanced induction by the WC On -CC1 adjuvant plus (WC+CC) vaccine.…”

Section: Introductionmentioning

confidence: 99%

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Adjuvant Effects of a CC Chemokine for Enhancing the Efficacy of an Inactivated Streptococcus agalactiae Vaccine in Nile Tilapia (Oreochromis niloticus)

Soontara,

Uchuwittayakul,

Kayansamruaj

et al. 2024

Vaccines

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In this study, the ability of a CC chemokine (On-CC1) adjuvant to enhance the efficacy of a formalin-killed Streptococcus agalactiae vaccine (WC) in inducing immune responses against S. agalactiae in Nile tilapia was investigated through immune-related gene expression analysis, enzyme-linked immunosorbent assay (ELISA), transcriptome sequencing, and challenge tests. Significantly higher S. agalactiae-specific IgM levels were detected in fish in the WC+CC group than in the WC alone or control groups at 8 days postvaccination (dpv). The WC vaccine group exhibited increased specific IgM levels at 15 dpv, comparable to those of the WC+CC group, with sustained higher levels observed in the latter group at 29 dpv and after challenge with S. agalactiae for 14 days. Immune-related gene expression analysis revealed upregulation of all target genes in the control group compared to those in the vaccinated groups, with notable differences between the WC and WC+CC groups at various time intervals. Additionally, transcriptome analysis revealed differential gene expression profiles between the vaccinated (24 and 96 hpv) and control groups, with notable upregulation of immune-related genes in the vaccinated fish. Differential gene expression (DGE) analysis revealed significant upregulation of immunoglobulin and other immune-related genes in the control group compared to those in the vaccinated groups (24 and 96 hpv), with distinct patterns observed between the WC and WC+CC vaccine groups. Finally, challenge with a virulent strain of S. agalactiae resulted in significantly higher survival rates for fish in the WC and WC+CC groups compared to fish in the control group, with a notable increase in survival observed in fish in the WC+CC group.

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