Comparative transcriptome analysis reveals the regulatory effects of exogenous auxin on lateral root development and tanshinone accumulation in Salvia miltiorrhiza

Zhang, Shuncang; Lin, Qing; Zheng, Yufeng; Wang, Wei; Zhao, Hongguang; Yang, Dongfeng

doi:10.1007/s00425-023-04193-1

Cited by 8 publications

(6 citation statements)

References 51 publications

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“…Cryptotanshinone (CTN) is a diterpenoid monomer and a lipophilic component extracted from the dried roots and rhizomes of the traditional Chinese herb S. miltiorrhiza ( Li et al, 2021 ; Zhang et al, 2023 ). CTN has been proven to possess various biological activities, including antioxidant ( Guo et al, 2022 ), anti-tumor ( Dalil et al, 2022 ), antibacterial ( Zhong et al, 2021 ), and anti-inflammatory effects ( Wu et al, 2020 ).…”

Section: Natural Products Modulating Ferroptosis For Intervention In ...mentioning

confidence: 99%

Induction of ferroptosis by natural products in non-small cell lung cancer: a comprehensive systematic review

Zhang,

Xia,

Wang

et al. 2024

Front. Pharmacol.

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Lung cancer is one of the leading causes of cancer-related deaths worldwide that presents a substantial peril to human health. Non-Small Cell Lung Cancer (NSCLC) is a main subtype of lung cancer with heightened metastasis and invasion ability. The predominant treatment approaches currently comprise surgical interventions, chemotherapy regimens, and radiotherapeutic procedures. However, it poses significant clinical challenges due to its tumor heterogeneity and drug resistance, resulting in diminished patient survival rates. Therefore, the development of novel treatment strategies for NSCLC is necessary. Ferroptosis was characterized by iron-dependent lipid peroxidation and the accumulation of lipid reactive oxygen species (ROS), leading to oxidative damage of cells and eventually cell death. An increasing number of studies have found that exploiting the induction of ferroptosis may be a potential therapeutic approach in NSCLC. Recent investigations have underscored the remarkable potential of natural products in the cancer treatment, owing to their potent activity and high safety profiles. Notably, accumulating evidences have shown that targeting ferroptosis through natural compounds as a novel strategy for combating NSCLC holds considerable promise. Nevertheless, the existing literature on comprehensive reviews elucidating the role of natural products inducing the ferroptosis for NSCLC therapy remains relatively sparse. In order to furnish a valuable reference and support for the identification of natural products inducing ferroptosis in anti-NSCLC therapeutics, this article provided a comprehensive review explaining the mechanisms by which natural products selectively target ferroptosis and modulate the pathogenesis of NSCLC.

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Section: Natural Products Modulating Ferroptosis For Intervention In ...mentioning

confidence: 99%

Induction of ferroptosis by natural products in non-small cell lung cancer: a comprehensive systematic review

Zhang,

Xia,

Wang

et al. 2024

Front. Pharmacol.

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“…Total RNA was extracted based on the instructions of FastPure® Universal Plant Total RNA Isolation Kit (Vazyme Biotech, China), and then converted into cDNA using a HiScript® III RT SuperMix for qPCR Kit (Vazyme Biotech, China). Gene expression was monitored via real-time quantitative PCR (qRT-PCR) followed the methods described in the previous study (Zhang et al 2023). Three biological replicates were set for each sample and relative expression was analyzed according to the 2 −△△Ct method and represented by their means ± SD.…”

Section: Plant Materialsmentioning

confidence: 99%

“…The determination of bioactive compounds content was conducted followed the methods described in our previous study (Zhang et al 2023). Brie y, the sample was dried at 45℃ to constant weight.…”

Section: Determination Of Bioactive Compounds Content With Hplcmentioning

confidence: 99%

“…The roots of RNAi-12, RNAi-18, and WT control were used for the RNA-seq analysis. The extraction of total RNA and construction of cDNA library for RNA-seq analysis were conducted according to the methods described in our previous study (Zhang et al 2023). Differential expression analysis between the control samples and RNAi samples (RNAi-12 and RNAi-18) was performed using the DESeq R package (1.10.1).…”

Section: Rna-seq Analysismentioning

confidence: 99%

“…Auxin is the rst discovered phytohormone and is relevant to various biological processes in plants (Perez-Henriquez and Yang 2023). Our previous study revealed that exogenous auxin in a suitable concentration (10 µΜ indole-3-aceticacid (IAA)) promoted the lateral root development of S. miltiorrhiza, and application of N-1-naphthylphthalamic acid (NPA, 20 µΜ) suppressed the growth and root development of S. miltiorrhiza (Zhang et al 2023). Besides, several transcription factors have been reported to be relevant to the auxin mediated root development in S. miltiorrhiza.…”

Section: Introductionmentioning

confidence: 99%

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SmTIR1 positively regulates the growth and root development of Salvia miltiorrhiza

Qiu,

Zheng,

Wang

et al. 2023

Plant Cell Tiss Organ Cult

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The roots of Salvia miltiorrhiza are widely used as medicinal materials in China and other East Asian countries. Root morphology and development are important factors that affect the quality and yield of this herb, but the molecular mechanism underlying root morphogenesis in S. miltiorrhiza remains elusive. Auxin is one of the main phytohormones that regulate root development in plants, and auxin receptor transport inhibitor response 1 (TIR1) plays pivotal roles in this biological process. In this study, SmTIR1 gene was cloned and transgenic S. miltiorrhiza plantlets were obtained to investigate the function of SmTIR1 in root development of S. miltiorrhiza. As the results shown, SmTIR1 positively regulated the growth and root development in S. miltiorrhiza. The SmTIR1 overexpression lines showed increased leaf number, root biomass, lateral root number, and lateral root diameter. The SmTIR1 RNAi lines showed the opposite morphological indices compared with the SmTIR1 overexpression lines. Besides, the contents of phenolic acids in leaves of SmTIR1 overexpression lines were remarkedly increased, but accumulation of bioactive compounds in roots of SmTIR1 transgenic S. miltiorrhiza was did not altered compared with the control. The RNA-seq analysis was performed and a total of 26 differential expressed genes in the auxin signaling pathway were identi ed in SmTIR1 RNAi lines, these genes may be relevant to the SmTIR1 regulated growth and root development in this species. Our results shed new light on the molecular mechanism of root morphogenesis of S. miltiorrhiza and is helpful to improve the quality of this herb with biological method in the production practice. Key MessageSmTIR1 showed essential roles in root development and the accumulation of phenolic acids in leaves of Salvia miltiorrhiza. It was a pivotal regulator of yield and quality of this herb.

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Genome-wide identification of the SAUR gene family and screening for SmSAURs involved in root development in Salvia miltiorrhiza

Wang,

Zheng,

Qiu

et al. 2024

Plant Cell Rep

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Comparative transcriptome analysis reveals the regulatory effects of exogenous auxin on lateral root development and tanshinone accumulation in Salvia miltiorrhiza

Cited by 8 publications

References 51 publications

Induction of ferroptosis by natural products in non-small cell lung cancer: a comprehensive systematic review

Induction of ferroptosis by natural products in non-small cell lung cancer: a comprehensive systematic review

SmTIR1 positively regulates the growth and root development of Salvia miltiorrhiza

Genome-wide identification of the SAUR gene family and screening for SmSAURs involved in root development in Salvia miltiorrhiza

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