Comparative transcriptome analysis reveals differentially expressed genes in the Asian citrus psyllid (Diaphorina citri) upon heat shock

Xiong, Ying; Liu, Xiaoqiang; Xiao, Ping-An; Tang, Guanghui; Liu, Shi-Huo; Lou, Binghai; Wang, Jinjun; Jiang, Hong‐Bo

doi:10.1016/j.cbd.2019.03.009

Cited by 23 publications

(20 citation statements)

References 29 publications

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“…It is interesting to note that HSPs participated in almost all the treatment but were heavily involved in IM25. HSPs are a family of proteins that can help to protect organisms from induced environmentally cellular damage induced by thermal stress and insecticides exposure [45][46][47]. Up-regulation of HSPs in this study corroborates these earlier findings, with only an exception in BCP35, where most HSPs were down-regulated.…”

Section: Discussionsupporting

confidence: 91%

Analysis of Differentially Expressed Transcripts in Apolygus lucorum (Meyer-Dür) Exposed to Different Temperature Coefficient Insecticides

Liu

Dou

et al. 2020

IJMS

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The existence of a temperature effect of insecticides frustrated the control of the green plant bug Apolygus lucorum (Meyer-Dür). Previous studies mostly focused on the application of insecticides, but the underlying mechanism remains incompletely understood. Here, we report a transcriptome profiling of A. lucorum treated by three kinds of temperature coefficient insecticides (TCIs) (positive TCI: imidacloprid, negative TCI: b-cypermethrin and non-effect TCI: phoxim) at 15 °C, 25 °C and 35 °C by using next- and third-generation RNA-Seq methods. A total of 34,739 transcripts were annotated from 277.74 Gb of clean data. There were more up-regulated transcripts than down-regulated transcripts in all three kinds of TCI treatments. Further Venn diagrams indicate the regulatory transcripts and regulatory modes were different at the three temperatures. The responses to imidacloprid involved more detox and stress response transcripts such as cytochrome P450 (CYP450), carboxylesterase (CarE) and catalase (CAT) at 35 °C, which was the case for beta-cypermethrin at 15 °C. UDP-glucuronyltransferase (UGT) and heat shock protein (HSP) transcripts were heavily involved, and thus deserve particular note in the temperature effect of insecticides. This high-confidence transcriptome atlas provides improved gene information for further study on the insecticide temperature effect related physiological and biochemical processes of A. lucorum.

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Section: Discussionsupporting

confidence: 91%

Analysis of Differentially Expressed Transcripts in Apolygus lucorum (Meyer-Dür) Exposed to Different Temperature Coefficient Insecticides

Liu

Dou

et al. 2020

IJMS

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“…We consider enrichment significant at P ≤ 0.05, as we were primarily interested in examining functional information for genes that were already stringently selected following conservative FDR correction, and GO enrichment P values do not conform to distributions typically required for FDR correction 96 . For ease of visualizing GO terms in presented results, GO lists were simplified using ReViGO 97 (allowed similarity = small), and then filtered for terms (with parent and child terms) previously identified as enriched in thermal stress studies in other organisms 58 , 98 – 100 . However, complete unfiltered GO results can be found in Datasets S1 E and S2 C, and intermediate datasets and R code can be found on DRYAD (10.5061/dryad.tmpg4f4wx).…”

Section: Methodsmentioning

confidence: 99%

Biogeographic parallels in thermal tolerance and gene expression variation under temperature stress in a widespread bumble bee

Pimsler

Oyen

Herndon

et al. 2020

Sci Rep

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Global temperature changes have emphasized the need to understand how species adapt to thermal stress across their ranges. Genetic mechanisms may contribute to variation in thermal tolerance, providing evidence for how organisms adapt to local environments. We determine physiological thermal limits and characterize genome-wide transcriptional changes at these limits in bumble bees using laboratory-reared Bombus vosnesenskii workers. We analyze bees reared from latitudinal (35.7–45.7°N) and altitudinal (7–2154 m) extremes of the species’ range to correlate thermal tolerance and gene expression among populations from different climates. We find that critical thermal minima (CTMIN) exhibit strong associations with local minimums at the location of queen origin, while critical thermal maximum (CTMAX) was invariant among populations. Concordant patterns are apparent in gene expression data, with regional differentiation following cold exposure, and expression shifts invariant among populations under high temperatures. Furthermore, we identify several modules of co-expressed genes that tightly correlate with critical thermal limits and temperature at the region of origin. Our results reveal that local adaptation in thermal limits and gene expression may facilitate cold tolerance across a species range, whereas high temperature responses are likely constrained, both of which may have implications for climate change responses of bumble bees.

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“…In fact, many studies have used RNAsequencing (RNA-Seq) to solve a wide variety of problems. Some studies focused on insect resistance to insecticide [30][31][32][33] , some on insect adaptability to extreme environments [34,35] , and others on selected genes, like chemosensory genes [36] . But there have been few studies on insect diapause using RNA-Seq.…”

Section: Introductionmentioning

confidence: 99%

High-throughput profiling of diapause regulated genes from Trichogramma dendrolimi, an important egg parasitoid

Zhang¹,

Du²,

Zhang³

et al. 2020

Preprint

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Background The parasitoid insect Trichogramma dendrolimi can successfully enter diapause at the prepupal stage. Thus, diapause is an efficient preservation method during the mass production of T. dendrolimi. Previous studies on diapause have focused mostly on ecological characteristics, so the molecular mechanism of diapause in T. dendrolimi is mostly unknown. In this study, we compared transcriptomes of diapause and non-diapause T. dendrolimi to identify genes involved in the development of diapause. Results Transcriptome sequencing was performed using different samples, including diapause prepupae, pupae after diapause, normal prepupae, and pupae. This initially yielded a total of 87,022 unigenes with an average length of 1,604 bp. By removing redundant sequences and those without significant BLAST hits, a non-redundant dataset was generated, containing 7,593 sequences with an average length of 3351 bp. Among the differentially expressed genes, 1,204 were specifically expressed during the diapause stage, and 820 were significantly up-regulated. The result of GO enrichment analysis revealed that binding, oxidation−reduction process, and ribosome biogenesis were significantly affected. Ten genes were selected for validation using a quantitative real-time PCR (qPCR). The changes showed the same trend between the qPCR and RNA-Seq results. Based on previous studies and our data, several genes were identified to be involved in diapause, including UDP-glucuronosyltransferase; Glutathione-S-transferase, ATGs, zinc finger protein genes, cell cycle-related genes, Trehalase, trehalose transporter, hsp68, p53, and DNA damage-regulated gene 1 (pdrg1), and genes related to lipid metabolism were also included. Conclusions In this study, we generated a great amount of transcriptome data from T. dendrolimi, providing a resource for gene function research. The diapause-related genes that we identified establish a valuable basis for future studies on the molecular mechanisms of diapause, not only for T. dendrolimi, but for other species as well.

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Comparative transcriptome analysis reveals differentially expressed genes in the Asian citrus psyllid (Diaphorina citri) upon heat shock

Cited by 23 publications

References 29 publications

Analysis of Differentially Expressed Transcripts in Apolygus lucorum (Meyer-Dür) Exposed to Different Temperature Coefficient Insecticides

Analysis of Differentially Expressed Transcripts in Apolygus lucorum (Meyer-Dür) Exposed to Different Temperature Coefficient Insecticides

Biogeographic parallels in thermal tolerance and gene expression variation under temperature stress in a widespread bumble bee

High-throughput profiling of diapause regulated genes from Trichogramma dendrolimi, an important egg parasitoid

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