Comparative Transcriptome Analysis Revealed the Freezing Tolerance Signaling Events in Winter Rapeseed (Brassica rapa L.)

Abstract

Data Availability Statement:The raw transcriptome reads have been deposited into the NCBI Short Read Archive (SRA) under accession number PRJNA782399. Conflicts of Interest:The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.Author Contributions: WW and YW conceived and designed this study. YW, HY, JS, YD, PX, and JH performed the experiments. WW, LD, GW and SZ analyzed the data. YW performed the bioinformatics analysis. WW and YW wrote the manuscript. All authors contributed to the article and approved the submitted version.

Effect of shading on physiological attributes and comparative transcriptome analysis of Camellia sinensis cultivar reveals tolerance mechanisms to low temperatures

“…According to various transcriptome data, several genes and transcription factors play an essential role in photosynthesis, plant hormone signal transduction, and mitogen-activated protein kinase signal cascades under different abiotic stresses ( Li et al., 2019 ; Ermilova, 2020 ; Ritonga and Chen, 2020 ; Aslam et al., 2022 ). In this study, the annotation of Gene Ontology and KEGG enrichment analysis demonstrated that low temperatures regulated the expression of several DEGs in the photosynthetic pathway of photosynthesis ( Wu et al., 2020 ) and activated numerous DEGs and transcription factors in signal transduction pathways ( Wang et al., 2018 ; Wu, 2022 ) under shade and no-shade control plants in November and December, respectively.…”

“…This indicates that under different shade groups, the higher chlorophyll concentration and more pigment-binding proteins are associated with their photons collected by photosystem II per unit. The light-harvesting complex of photosystem II is mainly composed of chlorophyll b ( Wu, 2022 ). In contrast, the low PAR, photochemical efficiency, and lower chlorophyll concentration in no-shade control plants indicate that cold temperatures might disrupt the membrane permeability in the cell compartment of tea leaves exposed to low temperatures.…”

“…Among them, plant hormone signal transduction is a crucial pathway for activating DEGs and transcription factors under different shading conditions ( Lorrain et al., 2008 ; Gommers et al., 2017 ). These DEGs and transcription factors are active during low temperatures ( Wu, 2022 ). In the present study, several critical DEGs and transcription factors were regulated in three shading groups compared with no-shade control plants in November and December during low temperatures.…”

“…Recently, a few studies were published on plant response to the shading on gene and transcriptional expression levels (Wu et al, 2020;Fang et al, 2022). The study of Wu et al (2022) revealed that under three shading conditions compared to no-shade control plants, the phenotypic, physiological, and photosynthetic DEGs were enhanced in Sambucus canadensis (Wu et al, 2020). The authors also observed that the DEGs linked with photosynthesis, secondary metabolites, and other hormones were affected in shading groups compared to no-shade control plants.…”

“…Recent research has shown that the MAPK cascade is also important in the biotic and abiotic stress responses of horticulture plants, including low temperatures. The genes and transcription factors are triggered in plant hormone signal transduction and mitogen-activated protein kinase signaling pathways ( Wu, 2022 ) to enhance plant immunity ( Li et al., 2019 ) and increase tolerance. Mitogen-activated protein kinases are required to integrate diverse intracellular signals transmitted by stress-induced secondary messengers.…”