Comparative Transcriptome Analysis Provides Novel Insights into the Effect of Lipid Metabolism on Laying of Geese

Abstract

The lower egg production of geese (20~60 eggs per year) compared with chicken and duck limits the development of the industry, while the yolk weight and fatty liver susceptibility of geese was higher than that of other poultry. Therefore, the relationship between lipid metabolism and the laying performance of geese remains to be explored. Phenotypically, we observed that the liver fat content of the high-, low-, and no-egg production groups decreased in turn, while the abdominal fat weight increased in turn. For transcriptional regulation, the KEGG pathways related to lipid metabolism were enriched in all pairwise comparisons of abdominal fat and liver through functional analysis. However, some KEGG pathways related to inflammation and the circadian rhythm pathway were enriched by DEGs only in abdominal fat and the liver, respectively. The DEGs in ovarian stroma among different groups enriched some KEGG pathways related to ovarian steroidogenesis and cell adhesion. Our research reveals that lipid metabolism regulated by the circadian rhythm of the liver may directly or indirectly affect ovarian function through the inflammation and hormone secretion of abdominal fat. These results offer new insights into the regulation mechanisms of goose reproductive traits.

Molecular Mechanisms of circRNA–miRNA–mRNA Interactions in the Regulation of Goose Liver Development

“…Although there are numerous reports on goose liver, most of these studies have focused on fatty liver [ 24 , 25 , 26 ], with few reports on the different developmental stages of goose liver. This study used RNA-seq technology to systematically identify the expression profiles of circRNAs and miRNAs in goose liver at three developmental stages (posthatching day 0, 10 weeks old, and 30 weeks old).…”

Role of SNPs located in the exon 9 of ATAPA1 gene on goose egg production

“…On the other hand, the researchers found some candidate genes were related to goose egg production by used transcriptome sequencing technology to compare the tissue at different laying levels ( Wu, et al, 2020 ; Ouyang et al, 2022 ), physiological stages ( Ouyang et al, 2020 ; Qin et al, 2021 ), and follicles development stages. However, whether these candidate genes have reliable molecular markers is unknown.…”