Comparative transcriptome analysis on candidate genes involved in lipid biosynthesis of developing kernels for three walnut cultivars in Xinjiang

Wang, Wenqiang; Wen, Hao; Jin, Qiang; Yu, Wenjun; Li, Gen; Wu, Minyu; Bai, Haotian; Shen, Lirong; Wu, Cuiyun

doi:10.1016/j.fshw.2022.04.020

Cited by 9 publications

(3 citation statements)

References 58 publications

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“…Despite having different gene expression patterns, seeds and carpels showed a synergistic relationship during seed development [105]. Similar studies have since been performed in other species [101,[106][107][108][109].…”

Section: Identification Of Differentially Expressed Genessupporting

confidence: 56%

Lipid Metabolism and Improvement in Oilseed Crops: Recent Advances in Multi-Omics Studies

Bu,

Fan,

et al. 2023

Metabolites

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Oilseed crops are rich in plant lipids that not only provide essential fatty acids for the human diet but also play important roles as major sources of biofuels and indispensable raw materials for the chemical industry. The regulation of lipid metabolism genes is a major factor affecting oil production. In this review, we systematically summarize the metabolic pathways related to lipid production and storage in plants and highlight key research advances in characterizing the genes and regulatory factors influencing lipid anabolic metabolism. In addition, we integrate the latest results from multi-omics studies on lipid metabolism to provide a reference to better understand the molecular mechanisms underlying oil anabolism in oilseed crops.

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Section: Identification Of Differentially Expressed Genessupporting

confidence: 56%

Lipid Metabolism and Improvement in Oilseed Crops: Recent Advances in Multi-Omics Studies

Bu,

Fan,

et al. 2023

Metabolites

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“…In walnut cultivar “Linzaoxiang”, the expression pattern of WRI1 aligned with the accumulation of oil, and WRI1 may play an important role in the synthesis of walnut oil [ 29 ]. Wang et al [ 54 ] identified five important TFs (WRI1, ABI3, FUS3, PKL and VAL1) which might highly regulate ACCase, KASII, LACS, FAD3 and LPAAT. In this study, the results of gene co-expression analysis showed that AP2/ERF and bHLH were the key TFs for the walnut oil accumulation during seed kernel development.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Genes Involved in Fatty Acid and Lipid Biosynthesis in Developing Walnut (Juglans regia L.) Seed Kernels from Qinghai Plateau

Shi

Zhang

2022

Plants

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Walnut (Juglans regia) is an important woody oil-bearing plant with high nutritional value. For better understanding of the underlying molecular mechanisms of its oil accumulation in the Qinghai Plateau, in this study we monitored walnut fruit development, and 15 cDNA libraries were constructed from walnut seed kernels collected at 72, 79, 93, 118 and 135 days after flowering (DAF). The candidate genes were identified using sequencing and expression analysis. The results showed that the oil content in the kernels increased dramatically in late July and reached the maximum value of 69% in mature seed. More than 90% of the oils were unsaturated fatty acids (UFAs) and linoleic acid (18:2) was the predominant UFA accumulated in mature seed. Differentially expressed genes (DEGs) in 15 KEGG pathways of lipid metabolism were detected. We identified 119 DEGs related to FA de novo biosynthesis (38 DEGs), FA elongation and desaturation (39 DEGs), triacylglycerol (TAG) assembly (24 DEGs), oil bodies (12 DEGs), and transcription factors (TFs, 6 DEGs). The abundantly expressed oleosins, caleosins and steroleosins may be important for timely energy reserve in oil bodies. Weighted gene coexpression network analysis (WGCNA) showed that AP2/ERF and bHLH were the key TFs, and were co-expressed with ACC1, α-CT, BCCP, MAT, KASII, LACS, FATA, and PDCT. Our transcriptome data will enrich public databases and provide new insights into functional genes related to the seed kernel lipid metabolism and oil accumulation in J. regia.

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“…Data shows that China’s walnut planting area and production reached 631,000 hectares and more than 2.5 million tons in 2019, respectively [ 4 ]. Walnut kernels are rich in nutrients, containing large amounts of protein, dietary fiber, carbohydrates, vitamins, fat, and amino acids, as well as trace elements such as potassium, iron, and calcium [ 5 , 6 , 7 ]. Walnut kernels have anti-inflammatory and antiseptic effects, protecting blood vessels, lowering blood pressure, and preventing cancer.…”

Section: Introductionmentioning

confidence: 99%

The Antioxidant Activities In Vitro and In Vivo and Extraction Conditions Optimization of Defatted Walnut Kernel Extract

Zhou,

Gong,

et al. 2023

Foods

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The objective of this study was to determine the antioxidant activities of defatted walnut kernel extract (DWE) and whole walnut kernel extract (WE) in vitro and in vivo. Three spectrophotometric methods, DPPH, ABTS, and FRAP, were used in in vitro experiments, and mice were used in in vivo experiments. In addition, response surface methodology (RSM) was used to optimize reflux-assisted ethanol extraction of DWE for maximum antioxidant activity and total phenolic content. The results of in vitro experiments showed that both extracts showed antioxidant activity; however, the antioxidant activity of DWE was higher than that of WE. Both extracts improved the mice’s oxidative damage status in in vivo studies. An ethanol concentration of 58%, an extraction temperature of 48 °C, and an extraction time of 77 min were the ideal parameters for reflux-assisted ethanol extraction of DWE. The results may provide useful information for further applications of defatted walnut kernels and the development of functional foods.

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Comparative transcriptome analysis on candidate genes involved in lipid biosynthesis of developing kernels for three walnut cultivars in Xinjiang

Cited by 9 publications

References 58 publications

Lipid Metabolism and Improvement in Oilseed Crops: Recent Advances in Multi-Omics Studies

Lipid Metabolism and Improvement in Oilseed Crops: Recent Advances in Multi-Omics Studies

Transcriptome Analysis of Genes Involved in Fatty Acid and Lipid Biosynthesis in Developing Walnut (Juglans regia L.) Seed Kernels from Qinghai Plateau

The Antioxidant Activities In Vitro and In Vivo and Extraction Conditions Optimization of Defatted Walnut Kernel Extract

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