Comparative transcriptome analysis of Yersinia pestis in response to hyperosmotic and high-salinity stress

Han, Yanping; Zhou, Dongsheng; Pang, Xin; Zhang, Ling; Song, Yajun; Tong, Zongzhong; Bao, Jingyue; Dai, Erfu; Wang, Jin; Guo, Zhaobiao; Zhai, Jing; Du, Zongmin; Wang, Xiaoyi; Wang, Jian; Huang, Peitang; Yang, Ruifu

doi:10.1016/j.resmic.2004.10.004

Cited by 45 publications

(44 citation statements)

References 30 publications

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“…This glucosecontaining polysaccharide may be utilized as a source of energy during stationary phase to prolong viability. On the other hand, induction of the operon responsible for glycogen accumulation in Yersinia pestis by hyperosmotic stress led to the proposal that the accumulation of glycogen could assist in restoring the cytoplasmic volume after an osmotic shock (20). We found that there was a high level of induction of glgA2, glgB2, and glgX2, three genes involved in glycogen metabolism located on the pSymB plasmid of S. meliloti; however, the corresponding chromosomal alleles were not affected.…”

Section: Resultsmentioning

confidence: 68%

“…Undecaprenyl pyrophosphate is required as a lipid carrier of glycosyl transfer in the biosynthesis of a variety of cell wall polysaccharides in bacteria, including LPS. This gene is also downregulated by hyperosmotic stress but not by NaCl in Y. pestis (20).…”

Section: Resultsmentioning

confidence: 94%

“…Although these stresses are similar, it has been shown that they can be recognized as different environmental stimuli by some organisms (20,27). On the other hand, the response to any stress condition can be divided into two phases: the initial reaction to sudden exposure and the subsequent cellular adaptation to prolonged growth under that condition.…”

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confidence: 99%

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Transcriptome Profiling Reveals the Importance of Plasmid pSymB for Osmoadaptation of Sinorhizobium meliloti

et al. 2006

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In this work, DNA microarrays were used to investigate genome-wide transcriptional responses of Sinorhizobium meliloti to a sudden increase in external osmolarity elicited by addition of either NaCl or sucrose to exponentially growing cultures. A time course of the response within the first 4 h after the osmotic shock was established. We found that there was a general redundancy in the differentially expressed genes after NaCl or sucrose addition. Both kinds of stress resulted in induction of a large number of genes having unknown functions and in repression of many genes coding for proteins with known functions. There was a strong replicon bias in the pattern of the osmotic stress response; whereas 64% of the upregulated genes had a plasmid localization, 85% of the downregulated genes were chromosomal. Among the pSymB osmoresponsive genes, 83% were upregulated, suggesting the importance of this plasmid for S. meliloti osmoadaptation. Indeed, we identified a 200-kb region in pSymB needed for adaptation to saline shock which has a high density of osmoregulated genes.

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Section: Resultsmentioning

confidence: 68%

Section: Resultsmentioning

confidence: 94%

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Transcriptome Profiling Reveals the Importance of Plasmid pSymB for Osmoadaptation of Sinorhizobium meliloti

et al. 2006

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“…Several transcriptome studies have shown that higBA2 transcription is induced by various stress conditions, hypersaline shock, the presence of streptomycin or chloramphenicol, and amino acid starvation (19,20,41,42), or during the stationary growth phase (5). HigB2 may thus participate in the adaptation of Y. pestis to its environment (19).…”

Section: Discussionmentioning

confidence: 99%

The Yersinia pestis Chromosome Encodes Active Addiction Toxins

et al. 2010

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Toxin-antitoxin (TA) loci consist of two genes in an operon, encoding a stable toxin and an unstable antitoxin. The expression of toxin leads to cell growth arrest and sometimes bacterial death, while the antitoxin prevents the cytotoxic activity of the toxin. In this study, we show that the chromosome of Yersinia pestis, the causative agent of plague, carries 10 putative TA modules and two solitary antitoxins that belong to five different TA families (HigBA, HicAB, RelEB, Phd/Doc, and MqsRA). Two of these toxin genes (higB2 and hicA1) could not be cloned in Escherichia coli unless they were coexpressed with their cognate antitoxin gene, indicating that they are highly toxic for this species. One of these toxin genes (higB2) could, however, be cloned directly and expressed in Y. pestis, where it was highly toxic, while the other one (hicA1) could not, probably because of its extreme toxicity. All eight other toxin genes were successfully cloned into the expression vector pBAD-TOPO. For five of them (higB1, higB3, higB5, hicA2, and tox), no toxic activity was detected in either E. coli or Y. pestis despite their overexpression. The three remaining toxin genes (relE1, higB4, and doc) were toxic for E. coli, and this toxic activity was abolished when the cognate antitoxin was coexpressed, showing that these three TA modules are functional in E. coli. Curiously, only one of these three toxins (RelE1) was active in Y. pestis. Cross-interaction between modules of the same family was observed but occurred only when the antitoxins were almost identical. Therefore, our study demonstrates that of the 10 predicted TA modules encoded by the Y. pestis chromosome, at least 5 are functional in E. coli and/or in Y. pestis. This is the first demonstration of active addiction toxins produced by the plague agent.

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“…If this hypothesis is true, then the specific internalin genes that are induced by salt stress might be important during gastrointestinal infection. In support of the importance of salt and osmotic stress as a stimulus that induces virulence gene expression, transcriptome analysis of Yersinia pestis identified a number of virulence genes that are up-regulated in response to salt and/or hyperosmotic stress conditions (26). Further, Heusipp et al reported that transcription of Yersinia enterocolitica rpoE, which encodes the alternative sigma factor E , is induced in vivo in infected mice and by osmotic stress, further supporting a link between salt stress and virulence, particularly as E has been shown to contribute to virulence for a number of bacteria (27).…”

Section: Discussionmentioning

confidence: 99%

The Alternative Sigma Factor σ^Band the Virulence Gene Regulator PrfA Both Regulate Transcription ofListeria monocytogenesInternalins

McGann

Wiedmann

Boor

2007

Appl Environ Microbiol

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Comparative transcriptome analysis of Yersinia pestis in response to hyperosmotic and high-salinity stress

Cited by 45 publications

References 30 publications

Transcriptome Profiling Reveals the Importance of Plasmid pSymB for Osmoadaptation of Sinorhizobium meliloti

Transcriptome Profiling Reveals the Importance of Plasmid pSymB for Osmoadaptation of Sinorhizobium meliloti

The Yersinia pestis Chromosome Encodes Active Addiction Toxins

The Alternative Sigma Factor σ^Band the Virulence Gene Regulator PrfA Both Regulate Transcription ofListeria monocytogenesInternalins

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Comparative transcriptome analysis of Yersinia pestis in response to hyperosmotic and high-salinity stress

Cited by 45 publications

References 30 publications

Transcriptome Profiling Reveals the Importance of Plasmid pSymB for Osmoadaptation of Sinorhizobium meliloti

Transcriptome Profiling Reveals the Importance of Plasmid pSymB for Osmoadaptation of Sinorhizobium meliloti

The Yersinia pestis Chromosome Encodes Active Addiction Toxins

The Alternative Sigma Factor σBand the Virulence Gene Regulator PrfA Both Regulate Transcription ofListeria monocytogenesInternalins

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The Alternative Sigma Factor σ^Band the Virulence Gene Regulator PrfA Both Regulate Transcription ofListeria monocytogenesInternalins