Comparative transcriptome analysis of two sugarcane varieties in response to diazotrophic plant growth promoting endophyte<i>Enterobacter roggenkampii</i>ED5

Guo, Dao-Jun; Li, Dong-Ping; Singh, Rajesh Kumar; Singh, Pratiksha; Sharma, Anjney; Qin, Ying; Khan, Qaisar; Song, Xiu–Peng; Malviya, Mukesh Kumar; Xing, Yong‐Xiu; Li, Yang–Rui

doi:10.1080/17429145.2021.2012608

Cited by 11 publications

(11 citation statements)

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“…Burkholderia anthina MYSP13 strain was also found in lateral root emergence locations indicating that colonization occurred in sugarcane root and shoot (Malviya et al, 2019). Recently endophytic plant growth promoting strain Enterobacter roggenkampii ED5 labeled with GFP had been effectively colonized the sugarcane tissues (Guo et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Sugarcane Root Transcriptome Analysis Revealed the Role of Plant Hormones in the Colonization of an Endophytic Diazotroph

et al. 2022

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Some sugarcane germplasms can absorb higher amounts of nitrogen via atmospheric nitrogen fixation through the bacterial diazotrophs. Most endophytic diazotrophs usually penetrate through the root, colonize inside the plant, and fix the nitrogen. To assess the plant’s bacterial association during root colonization, strain GXS16 was tagged with a plasmid-bear green fluorescent protein (GFP) gene. The results demonstrated that the strain can colonize roots all the way to the maturation zone. The strain GXS16 showed maximum nitrogenase enzyme activity at pH 8 and 30°C, and nitrogenase activity is less affected by different carbon sources. Further, strain GXS16 colonization response was investigated through plant hormones analysis and RNAseq. The results showed that the bacterial colonization gradually increased with time, and the H2O2 and malondialdehyde (MDA) content significantly increased at 1 day after inoculation. There were no substantial changes noticed in proline content, and the ethylene content was detected initially, but it decreased with time. The abscisic acid (ABA) content showed significant increases of 91.9, 43.9, and 18.7%, but conversely, the gibberellin (GA3) content decreased by 12.9, 28.5, and 45.2% at 1, 3, and 5 days after inoculation, respectively. The GXS16 inoculation significantly increased the activities of catalase (CAT), superoxide dismutase (SOD), polyphenol oxidase (PPO), ascorbate peroxidase (APX), and glutathione reductase (GR) at different timepoint. In contrast, the peroxisome (POD) activity had no changes detected during the treatment. In the case of RNAseq analysis, 2437, 6678, and 4568 differentially expressed genes (DEGs) were identified from 1, 3, and 5 days inoculated root samples, and 601 DEGs were shared in all samples. The number or the expression diversity of DEGs related to ethylene was much higher than that of ABA or GA, which indicated the critical role of ethylene in regulating the sugarcane roots response to GXS16 inoculation.

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Section: Discussionmentioning

confidence: 99%

Sugarcane Root Transcriptome Analysis Revealed the Role of Plant Hormones in the Colonization of an Endophytic Diazotroph

et al. 2022

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“…A recent study has shown that maize plants inoculated with A. brasilense sp245 or H. seropedicae HRC54 have higher expression of NR and NiR genes, respectively, when compared to non-inoculated plants [ 82 ]. Sugarcane plants inoculated with Enterobacter roggenkampii ED5, as well as wheat, maize, and cucumber plants inoculated with Paenibacillus beijingensis BJ-18, have shown higher expression of NR genes, indicating that these bacteria might stimulate nitrate reductase activity in these plants [ 76 , 83 ].…”

Section: Molecular Mechanisms Involved In the Association Of Plant An...mentioning

confidence: 99%

“…However, the success of this association may be dependent on the plant and bacteria genotypes as well as the N content present in the soil [ 76 , 79 , 83 ]. Several studies have shown that inoculation with diazotrophic bacteria may not influence nitrogen metabolism, although a greater accumulation of nutrients, growth, and development of plants can be observed [ 11 , 77 , 88 , 92 ].…”

Section: Molecular Mechanisms Involved In the Association Of Plant An...mentioning

confidence: 99%

The Impact of Non-Nodulating Diazotrophic Bacteria in Agriculture: Understanding the Molecular Mechanisms That Benefit Crops

Thiebaut

Urquiaga

Rosman

et al. 2022

IJMS

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Agriculture is facing increasing challenges with regard to achieving sustainable growth in productivity without negatively impacting the environment. The use of bioinoculants is emerging as a sustainable solution for agriculture, especially bioinoculants based on diazotrophic bacteria. Brazil is at the forefront of studies intended to identify beneficial diazotrophic bacteria, as well as in the molecular characterization of this association on both the bacterial and plant sides. Here we highlight the main advances in molecular studies to understand the benefits brought to plants by diazotrophic bacteria. Different molecular pathways in plants are regulated both genetically and epigenetically, providing better plant performance. Among them, we discuss the involvement of genes related to nitrogen metabolism, cell wall formation, antioxidant metabolism, and regulation of phytohormones that can coordinate plant responses to environmental factors. Another important aspect in this regard is how the plant recognizes the microorganism as beneficial. A better understanding of plant–bacteria–environment interactions can assist in the future formulation of more efficient bioinoculants, which could in turn contribute to more sustainable agriculture practices.

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“…But, other rice cultivars, such as 9311, TKM9 and IR36, have also been used ( Chen et al, 2015 ; Xie et al, 2017 ; Rekha et al, 2018 ). Besides rice, other plants such as wheat, sugarcane, and tobacco have been used in these studies ( Camilios-Neto et al, 2014 ; Paungfoo-Lonhienne et al, 2016 ; Liu et al, 2020 ; Malviya et al, 2020 ; Guo et al, 2022 ). Compared to the hosts, there is much more variability in the PGPB used in these studies.…”

Section: Transcriptomic Studies Have Revealed Key Gene Expression Pat...mentioning

confidence: 99%

“…While results from these studies are encouraging, similar studies investigating the interactions between PGPB and different crops need to be pursued. In recent years, there have been some studies using genetic and molecular approaches in crops such as rice, wheat, and sugarcane to investigate these interactions at the host level, which is encouraging ( Chen and Zhu, 2013 ; Camilios-Neto et al, 2014 ; Chen et al, 2015 ; Paungfoo-Lonhienne et al, 2016 ; Xie et al, 2017 ; Rekha et al, 2018 ; Wu et al, 2018 ; Brusamarello-Santos et al, 2019 ; King et al, 2019 ; Thomas et al, 2019 ; Liu et al, 2020 ; Malviya et al, 2020 ; Cook et al, 2022 ; Guo et al, 2022 ; Wiggins et al, 2022 ; Xu et al, 2022 ). This mini-review focuses on key findings from some of these studies and discusses what lies ahead.…”

Section: Introductionmentioning

confidence: 99%

What do we know from the transcriptomic studies investigating the interactions between plants and plant growth-promoting bacteria?

Mukherjee

2022

Front. Plant Sci.

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Major crops such as corn, wheat, and rice can benefit from interactions with various plant growth-promoting bacteria (PGPB). Naturally, several studies have investigated the primary mechanisms by which these PGPB promote plant growth. These mechanisms involve biological nitrogen fixation, phytohormone synthesis, protection against biotic and abiotic stresses, etc. Decades of genetic and biochemical studies in the legume-rhizobia symbiosis and arbuscular mycorrhizal symbiosis have identified a few key plant and microbial signals regulating these symbioses. Furthermore, genetic studies in legumes have identified the host genetic pathways controlling these symbioses. But, the same depth of information does not exist for the interactions between host plants and PGPB. For instance, our knowledge of the host genes and the pathways involved in these interactions is very poor. However, some transcriptomic studies have investigated the regulation of gene expression in host plants during these interactions in recent years. In this review, we discuss some of the major findings from these studies and discuss what lies ahead. Identifying the genetic pathway(s) regulating these plant-PGPB interactions will be important as we explore ways to improve crop production sustainably.

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Comparative transcriptome analysis of two sugarcane varieties in response to diazotrophic plant growth promoting endophyteEnterobacter roggenkampiiED5

Cited by 11 publications

References 64 publications

Sugarcane Root Transcriptome Analysis Revealed the Role of Plant Hormones in the Colonization of an Endophytic Diazotroph

Sugarcane Root Transcriptome Analysis Revealed the Role of Plant Hormones in the Colonization of an Endophytic Diazotroph

The Impact of Non-Nodulating Diazotrophic Bacteria in Agriculture: Understanding the Molecular Mechanisms That Benefit Crops

What do we know from the transcriptomic studies investigating the interactions between plants and plant growth-promoting bacteria?

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