Comparative transcriptome analysis of the calcium signaling and expression analysis of sodium/calcium exchanger in Aspergillus cristatus

Abstract

Aspergillus cristatus develops into various stages under different Na concentrations:the sexual stage in 0.5 M NaCl and asexual development stage in 3 M NaCl. In order to explore whether the Ca 2+ signaling pathway in A. cristatus responded to the changes in the salt stress, we analyzed the gene expression levels in A. cristatus respectively cultured in 0.5 M NaCl and 3 M NaCl. According to the BLAST analysis results, we identified 25 Ca 2+ -signaling proteins in A. cristatus. The expression levels of most genes involved in the Ca 2+ -signaling pathway in A. cristatus cultured in different salt concentrations showed significant differences, indicating that the Ca 2+ signaling pathway was involved in the response to the changes in the salt stress. In yeasts, only calcium ion influx proteins were reported to be involved in the response to the changes in the salt stress. So far, the protein for the exchanger of calcium/sodium ions has not been reported. Therefore, we obtained the sodium/calcium exchanger (termed NCX) proteins from the KEGG Database. The ncx gene of A. cristatus was cloned and characterized. The full length of ncx gene is 3055 bp, including a 2994-bp open reading frame encoding 994 amino acids. The expression levels of ncx in the sexual development stage and asexual development stage were respectively ∼8.94 times and ∼2.57 times of that in the hyphal formation stage. Therefore, we suggested that ncx gene was up-regulated to resist the sodium stress. The study results provide the basis for further exploring the Ca 2+ -signaling mechanism and ion exchanger mechanism.

Sequencing and phylogenetic analysis of mitochondrial genome of Aspergillus cristatus

“…Moreover, these can also be used to treat herbal medicine residues to produce anthraquinones with medicinal value (Kong et al 2019), hence, they are an important microbial resource. In addition, A. cristatus present completely different reproductive patterns under different osmotic pressures, and can be used as a good strain to study the mechanism of asexual and sexual reproduction of fungi (Ren et al 2018;Yongyi et al 2016). To date, the research on the mitochondrial genome information of A. cristatus has not been reported.…”

Caracterização de fatores de transcrição de Aspergillus fumigatus importantes para respostas a diferentes estresses

“…As células eucarióticas possuem uma gama complexa de canais permeáveis, bombas, transportadores e proteínas sinalizadoras de Ca 2+ (Matheos et al, 1997;Ren et al, 2017 (Chin et al,1998;Kramer et al, 2003;Parsons et al, 2007;Reedy et al, 2010), a memória (Mansuy et al,1998;Weitlauf and Winder, 2001;Reedy et al, 2010), a ativação de células-T (Clipstone e Crabtree, 1992;Reedy et al, 2010) e a apoptose (Krebs, 1998;Saito et al,2000;Shibasaki and McKeon, 1995;Wang et al, 1999;Reedy et al, 2010). Em Cryptococus neoformans, a atividade da calcineurina é essencial para o crescimento do fungo na temperatura corporal de um mamífero, à 37 °C (Liu et al, 2006), mas não à temperatura ambiente.…”