Comparative Transcriptome Analysis of the Skin-Specific Accumulation of Anthocyanins in Black Peanut (<i>Arachis hypogaea</i> L.)

Huang, Jinyong; Xing, Minghui; Li, Yan; Cheng, F. H.; Gu, Huihui; Yue, Caipeng; Zhang, Yanjie

doi:10.1021/acs.jafc.8b05915

Cited by 26 publications

(27 citation statements)

References 64 publications

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“…Anthocyanins are the main water-soluble pigments widespread in many flowering plants. The red, blue, and purple colors found in various plant tissues, including flowers, leaves, fruits, and seeds, are mostly attributed to the accumulation of anthocyanins 1–3 . In addition to their well-known physiological functions of serving as pollinator and seed disperser attractants, anthocyanins also play essential roles in protecting plant tissues from being damaged by high light, UV radiation, cold, drought, nutrient deficiency, and pathogen attack 4 ; anthocyanins also act as important health-promoting supplements in the human daily diet.…”

Section: Introductionmentioning

confidence: 99%

Metabolic and molecular analysis of nonuniform anthocyanin pigmentation in tomato fruit under high light

Zhang

et al. 2019

Hortic Res

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Pigment intensity and patterns are important factors that determine the nutritional and market values of tomato fruits. The acropetal manner of light-dependent anthocyanin accumulation with the highest levels at the stem end of the fruit makes Pro35S:BrTT8 tomato plants an ideal system for investigating the effects of light intensity on anthocyanin biosynthesis. Extensive transcript analyses indicate that anthocyanin pigmentation in Pro35S:BrTT8 plants under high light might be coordinately regulated by the exogenous protein BrTT8 and endogenous proteins SlAN2 and SlMYBL2. Furthermore, yeast two-hybrid assays showed that BrTT8 could interact efficiently with SlAN2, SlMYBL2, and SlAN11. Moreover, the physical interaction between BrTT8 and SlAN2 was validated by FRET. Simultaneous overexpression of SlAN2 and BrTT8 activated significant anthocyanin biosynthesis in infiltrated tobacco leaves. In addition, the ability of SlMYBL2 to suppress anthocyanin accumulation was also demonstrated in infiltrated tobacco leaves. Altogether, these results prove that tissue-specific assemblage of the heterogeneous MYB-bHLH-WD40 complex consisting of SlAN2, BrTT8 and SlAN11 triggers nonuniform anthocyanin accumulation in tomato fruit under high light. Additionally, it is proposed that a negative-feedback loop fulfilled by SlMYBL2 also participates in the regulation of anthocyanin production.

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Section: Introductionmentioning

confidence: 99%

Metabolic and molecular analysis of nonuniform anthocyanin pigmentation in tomato fruit under high light

Zhang

et al. 2019

Hortic Res

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“…The RNA samples of drought-stressed leaf were used for qRT-PCR analysis following the method described in previous studies [24]. Gene-specific qRT-PCR primers were designed using the Primer 5 software and listed in Additional file 1: Table S1.…”

Section: Methodsmentioning

confidence: 99%

Physiological and transcriptome analyses of Opisthopappus taihangensis in response to drought stress

Yang

Xing

et al. 2019

Cell Biosci

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Background Water scarcity is considered to be a severe environmental constraint to plant survival and productivity. Studies on drought-tolerant plants would definitely promote a better understanding of the regulatory mechanism lying behind the adaptive response of plants to drought. Opisthopappus taihangensis (ling) shih is a typical drought-tolerant perennial plant species endemically distributed across the Taihang Mountains in China, but the underlying mechanism for drought tolerance of this particular species remains elusive. Results To mimic natural drought stress, O. taihangensis plants were treated with two different concentrations (25% and 5%) of polyethylene glycol (PEG6000), which represent the H group (high salinity) and the L group (low salinity), respectively. The physiological characteristics of these two groups of plants, including relative water content maintenance (RWC), proline content and chlorophyll content were assessed and compared with plants in the control group (CK), which had normal irrigation. There was not a significant difference in RWC when comparing plants in the L group with the control group. Proline was accumulated to a higher level, and chlorophyll content was decreased slightly in plants under low drought stress. In plants from the H group, a lower RWC was observed. Proline was accumulated to an even higher level when compared with plants from the L group, and chlorophyll content was further reduced in plants under high drought stress. Transcriptomic analysis was carried out to look for genes that are differentially expressed (DEGs) in O. taihangensis plants coping adaptively with the two levels of drought stress. A total of 23,056 genes are differentially expressed between CK and L, among which 12,180 genes are up-regulated and 10,876 genes are down-regulated. Between H and L, 6182 genes are up-regulated and 1850 genes are down-regulated, which gives a total of 8032 genes. The highest number of genes, that are differentially expressed, was obtained when a comparison was made between CK and H. A total of 43,074 genes were found to be differentially expressed with 26,977 genes up-regulated and 16,097 genes down-regulated. Further analysis of these genes suggests that many of the up-regulated genes are enriched in pathways involved in amino acid metabolism. Besides, 39 transcription factors (TFs) were found to be continuously up-regulated with the increase of drought stress level. Conclusion Taken together, the results indicate that O. taihangensis plants are able to live adaptively under drought stress by responding physiologically and regulating the expression of a substantial number of drought-responsive genes and TFs to avoid adverse effects. Electronic supplementary material The online version of this article (10.1186/s13578-019-0318-7) contains supplementary materia...

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“…This shows that more delphinium is produced in the black fruit than cyanidin. Previous studies have shown that LrDFR and LrFLS share the same upstream substrates (e.g., DHM, DHQ, and DHK) and take a large proportion of total flavonoids in plants (Huang et al, 2019). In black fruits, the rate-limiting enzyme LrDFR specifically binds DHQ to synthesize delphinidin and its derivatives and has little binding to other substrates.…”

Section: Degs Involved In Flavonoid Metabolism Between Two Fruit Varimentioning

confidence: 99%

Transcriptome and Flavonoids Metabolomic Analysis Identifies Regulatory Networks and Hub Genes in Black and White Fruits of Lycium ruthenicum Murray

Fan

Qin

et al. 2020

Front. Plant Sci.

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Lycium ruthenicum Murry. is a highly nutritional cash crop due to its fruit abundant anthocyanins. To understand the complex metabolic networks underlying the color formation in black and white fruits of L. ruthenicum, we conducted transcriptome and flavonoid metabolic profiling to identify the candidate genes possibly involved in flavonoid biosynthesis. As a result, 147 flavonoids were identified and there was almost no anthocyanin in white fruits, while luteolin, kaempferol, and quercetin derivatives showed markedly higher abundance. Furthermore, applying weighted gene co-expression network analyses, 3 MYB, 2 bHLH, 1WRKY and 1 NAC transcription factor, associated with anthocyanin biosynthesis were identified. A bHLH transcription factor, LrAN1b showed the greatest correlations with anthocyanin accumulation with no expression in white fruits. In addition, gene function analysis and qRT-PCR experiments identified a new activated anthocyanin MYB transcription factor designed as LrAN2-like. Yeast two-hybrid and transient tobacco overexpression experiments showed that LrAN1b could interact with LrAN2-like and LrAN11 to form MBW complex to activate the anthocyanin pathway. The yeast one-hybrid experiment indicated that LrAN2-like bonded anthocyanin structural gene LrDFR and LrANS promoters. Heterologous expression of LrAN1b in tobacco can significantly increase the anthocyanin content of tobacco florals and capsules, and activate anthocyanin synthesis related genes. Taken together, an anthocyanin regulatory network model in L. ruthenicum fruit was proposed firstly and we speculate that the white fruit phenotype was due to abnormal expression of LrAN1b. The findings provide new insight into the underlying mechanism of flavonoids, laying the foundation for future functional and molecular biological research in L. ruthenicum.

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Comparative Transcriptome Analysis of the Skin-Specific Accumulation of Anthocyanins in Black Peanut (Arachis hypogaea L.)

Cited by 26 publications

References 64 publications

Metabolic and molecular analysis of nonuniform anthocyanin pigmentation in tomato fruit under high light

Metabolic and molecular analysis of nonuniform anthocyanin pigmentation in tomato fruit under high light

Physiological and transcriptome analyses of Opisthopappus taihangensis in response to drought stress

Transcriptome and Flavonoids Metabolomic Analysis Identifies Regulatory Networks and Hub Genes in Black and White Fruits of Lycium ruthenicum Murray

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