Comparative transcriptome analysis of the ovary and testis of the lacustrine goby (Gobiopterus lacustris)

Abstract

ABSTRCTLacustrine goby (Gobiopterus lacustris) belongs to a genus of gobies that are small in size and endemic to freshwater, brackish waters or coastal environments around the Indian and Pacific oceans. To date, there are no genomic or transcriptomic studies on G. lacustris. Here, we constructed gonadal transcriptomes of G. lacustris for the first time and identified genes that may be involved in gonadal development and reproduction. In total, 60,657,644 and 52,016,136 clean reads were obtained from ovary and testis, respectively, using Illumina sequencing. Reads were assembled into 62,573 unigenes with N50 value of 3,082 bp and a mean length of 1,869 bp. A total of 47,891 (76.53%) unigenes were annotated in at least one of the seven databases that were used in this study. In addition, 38,550 SSRs (simple sequence repeat, microsatellite) were identified from 20,517 SSR containing sequences. Gene expression patterns in the testis and ovary were compared, and 10,954 DEGs (differentially expressed genes) were identified. Of these genes, 2,383 were up-regulated in the testis and 8,571 were up-regulated in the ovary. RT-qPCR analysis of 14 selected genes showed patterns consistent with the transcriptome results. Numerous DEGs involved in gonadal development and gametogenesis were identified, including foxl2, dmrt1, cyp19a1a, inha, inhb, sycp2, zglp1, tdrp, zps and esra. Using GO and KEGG enrichment analyses, pathways involving regulation of gonadal development and gametogenesis also identified. This work represents the first gonadal transcriptomic analysis of G. lacustris and provides a valuable dataset for future research on the genes involved in reproduction of G. lacustris.

Morphological characteristics of sex dimorphism in Gobiopterus sp. (Gobiiformes: Oxudercidae) from Ranu Grati Lake, Pasuruan District, East Java, Indonesia

“…The differences in the morphology of male and female fish can be seen in Figure 2. This is in accordance with the statement of Dong et al (2019), that gobiopterus fish can be differentiated by sex through their morphological characteristics. Observation of fish sexual dimorphism can be done by observing the shape of the caudal fin, body shape, and body abdominal shape.…”

Transcriptomics‐based systematic identification and tissue‐specific distribution of cytochrome P450 genes in Carassius auratus

“…However, there is little information on the tissue‐specific role of CYP27 in fish. The newest finding has shown that cyp27a1 is highly expressed in the sexually mature testis of the lacustrine goby ( Gobiopterus lacustris ), and cyp27c1 is highly expressed in the ovary (Dong et al, 2021). As for cyp46a1, it has been demonstrated that its encoded enzyme is responsible for cholesterol elimination from the brain in hamster (Mast et al, 2010) and cavefish (Meng, Wang, et al, 2018; Meng, Zhao, et al, 2018).…”