Comparative transcriptome analysis of sex pheromone glands of two sympatric lepidopteran congener species

Jung, Chung Ryul; Kim, Yonggyun

doi:10.1016/j.ygeno.2014.02.009

Cited by 22 publications

(13 citation statements)

References 37 publications

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“…In moths, changes in enzymes involved in the synthesis pathway of pheromones do produce different pheromones in closely related species [20, 110, 111]. In L. longipalpis it is conceivable that an analogous process is occurring.…”

Section: Introductionmentioning

confidence: 99%

The Lutzomyia longipalpis complex: a brief natural history of aggregation-sex pheromone communication

et al. 2016

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In this paper we review the natural history of pheromone communication and the current diversity of aggregation-sex pheromones in the sand fly Lutzomyia longipalpis. This species complex is the main vector of Leishmania infantum, the agent of visceral leishmaniasis in the Americas. The identification of variation in pheromone chemotypes combined with molecular and sound analyses have all contributed to our understanding of the extent of divergence among cryptic members of this complex. The importance of chemical signals as pre-mating barriers and drivers of speciation is discussed. Moreover, the importance of aggregation-sex pheromones as sexually selected signals is highlighted with evidence from the literature suggesting their potential role in species and mate recognition as well as mate assessment. The distinct evolutionary forces possibly involved are briefly reviewed and discussed in the context of this intriguing insect.

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Section: Introductionmentioning

confidence: 99%

The Lutzomyia longipalpis complex: a brief natural history of aggregation-sex pheromone communication

et al. 2016

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“…Despite the economic importance of G. molesta , at the time of this research, only a limited number of studies had been conducted at the molecular level, including the sequencing of the mitochondrial genome (Gong et al 2012), characterization of two general odorant binding protein genes (Zhang et al 2012), population genetic structure (Kirk et al 2013), transcriptome analysis of sex pheromone glands (Jung and Kim 2014), and characterization of two heat shock proteins (Chen et al 2014). Although these studies generated rich genomic and EST sequence data for G. molesta , it lacked sequence information related to detoxification or other insecticide related genes.…”

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confidence: 99%

Transcriptome Analysis and Identification of Major Detoxification Gene Families and Insecticide Targets in Grapholita Molesta (Busck) (Lepidoptera: Tortricidae)

Guo¹,

Chai²,

Zhang³

et al. 2017

Journal of Insect Science

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The oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae), is an important pest of most stone and pome fruits and causes serious damage to the fruit industry worldwide. This insect pest has been primarily controlled through the application of insecticides; as a result, G. molesta has developed resistance to many different types of insecticides. To identify detoxification genes, we have, de novo, sequenced the transcriptome of G. molesta (Lepidoptera: Tortricidae) and yielded 58,970 unigenes of which 26,985 unigenes matched to known proteins. In total, 2,040 simple sequence repeats have been identified. The comprehensive transcriptome data set has permitted us to identify members of important gene families related to detoxification in G. molesta, including 77 unigenes of putative cytochrome P450s, 28 of glutathione S-transferases, 46 of Carboxylesterases, and 31 of insecticide targets. Orthologs of some of these unigenes have shown to play a pivotal role in insecticide resistance in other insect species and those unigenes likely have similar functions in G. molesta.

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“…This Transcriptome Shotgun Assembly project has been deposited at DDBJ/EMBL/GenBank under the accession GBXH00000000. In comparison with previously reported PG transcriptome/EST data [ 37 – 41 ], this pooled assembly of E. cautella PG sequences has the second greatest data volume and sequence lengths (Additional file 2 : Table S2).…”

Section: Resultsmentioning

confidence: 79%

“…These approaches are particularly relevant when no genomic data are available for the target species [ 36 ]. Over the past 5 years, RNA sequencing data on the insect pheromone gland amassed rapidly [ 37 – 41 ]. In the present study, using the Illumina sequencing approach, we constructed the transcriptome dataset of the PG of E. cautella and identified genes with putative roles in pheromone biosynthesis, transport and degradation.…”

Section: Introductionmentioning

confidence: 99%

Genes involved in sex pheromone biosynthesis of Ephestia cautella, an important food storage pest, are determined by transcriptome sequencing

et al. 2015

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BackgroundInsects use pheromones, chemical signals that underlie all animal behaviors, for communication and for attracting mates. Synthetic pheromones are widely used in pest control strategies because they are environmentally safe. The production of insect pheromones in transgenic plants, which could be more economical and effective in producing isomerically pure compounds, has recently been successfully demonstrated. This research requires information regarding the pheromone biosynthetic pathways and the characterization of pheromone biosynthetic enzymes (PBEs). We used Illumina sequencing to characterize the pheromone gland (PG) transcriptome of the Pyralid moth, Ephestia cautella, a destructive storage pest, to reveal putative candidate genes involved in pheromone biosynthesis, release, transport and degradation.ResultsWe isolated the E. cautella pheromone compound as (Z,E)-9,12-tetradecadienyl acetate, and the major pheromone precursors 16:acyl, 14:acyl, E14-16:acyl, E12-14:acyl and Z9,E12-14:acyl. Based on the abundance of precursors, two possible pheromone biosynthetic pathways are proposed. Both pathways initiate from C16:acyl-CoA, with one involving ∆14 and ∆9 desaturation to generate Z9,E12-14:acyl, and the other involving the chain shortening of C16:acyl-CoA to C14:acyl-CoA, followed by ∆12 and ∆9 desaturation to generate Z9,E12-14:acyl-CoA. Then, a final reduction and acetylation generates Z9,E12-14:OAc. Illumina sequencing yielded 83,792 transcripts, and we obtained a PG transcriptome of ~49.5 Mb. A total of 191 PBE transcripts, which included pheromone biosynthesis activating neuropeptides, fatty acid transport proteins, acetyl-CoA carboxylases, fatty acid synthases, desaturases, β-oxidation enzymes, fatty acyl-CoA reductases (FARs) and fatty acetyltransferases (FATs), were selected from the dataset. A comparison of the E. cautella transcriptome data with three other Lepidoptera PG datasets revealed that 45 % of the sequences were shared. Phylogenetic trees were constructed for desaturases, FARs and FATs, and transcripts that clustered with the ∆14, ∆12 and ∆9 desaturases, PG-specific FARs and potential candidate FATs, respectively, were identified. Transcripts encoding putative pheromone degrading enzymes, and candidate pheromone carrier and receptor proteins expressed in the E. cautella PG, were also identified.ConclusionsOur study provides important background information on the enzymes involved in pheromone biosynthesis. This information will be useful for the in vitro production of E. cautella sex pheromones and may provide potential targets for disrupting the pheromone-based communication system of E. cautella to prevent infestations.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1710-2) contains supplementary material, which is available to authorized users.

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Comparative transcriptome analysis of sex pheromone glands of two sympatric lepidopteran congener species

Cited by 22 publications

References 37 publications

The Lutzomyia longipalpis complex: a brief natural history of aggregation-sex pheromone communication

The Lutzomyia longipalpis complex: a brief natural history of aggregation-sex pheromone communication

Transcriptome Analysis and Identification of Major Detoxification Gene Families and Insecticide Targets in Grapholita Molesta (Busck) (Lepidoptera: Tortricidae)

Genes involved in sex pheromone biosynthesis of Ephestia cautella, an important food storage pest, are determined by transcriptome sequencing

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