Comparative transcriptome analysis in Triticum aestivum infecting wheat dwarf virus reveals the effects of viral infection on phytohormone and photosynthesis metabolism pathways

Abstract

Wheat dwarf virus (WDV), a mastrevirus transmitted by the leafhopper Psammotettix alienus, causes a severe disease in cereal crops. Typical symptoms of wheat plants infected by WDV are yellowing and severe dwarfing. In this present study, RNA-Seq was used to perform gene expression analysis in wheat plants in response to WDV infection. Comparative transcriptome analysis indicated that a total of 1042 differentially expressed genes (DEGs) were identified in the comparison between mock and WDV-inoculated wheat plants. Genomes ontology (GO) annotation revealed a number of DEGs associated with different biological processes, such as phytohormone metabolism, photosynthesis, DNA metabolic process, response to biotic stimulus and defense response. Among these, DEGs involved in phytohormone and photosynthesis metabolism and response pathways were further enriched and analyzed, which indicated that hormone biosynthesis, signaling and chloroplast photosynthesis-related genes might play an important role in symptom development after WDV infection. These results illustrate the dynamic nature of the wheat-WDV interaction at the transcriptome level and confirm that symptom development is a complex process, providing a solid foundation to elucidate the pathogenesis of WDV.

Plant responses to geminivirus infection: guardians of the plant immunity

“…The downregulation of SAUR72 suggested the possible roles of auxins in antiviral response. Similarly, two-component response regulator encoding ORR22 and ORR4 genes that are involved in cytokinin signalling pathways were upregulated and downregulated, respectively [142]. TGMV TrAP protein and spinach curly top virus (SCTV) C2-encoded TrAP protein expression resulted in inhibition of activity of ADK, a regulator of primary cytokinin responsive genes.…”

Comparative Transcriptome Analysis of CMV or 2b-Deficient CMV-Infected dcl2dcl4 Reveals the Effects of Viral Infection on Symptom Induction in Arabidopsis thaliana

“…Systematical infection with CMV or CMV mutants brings about some common physiological changes in host plants. Previous studies revealed that the virus infection can modify photosynthesis and disturb chloroplast components and functions [ 49 , 50 , 51 , 52 ], and coat protein of CMV is responsible for the chlorosis in infected leaves [ 51 ]. Indeed, we found numerous genes implicated in photosynthesis and chloroplast organization reduced expression pattern in CMV-infected plants ( Figures S2–S7 ).…”

OMICs, Epigenetics, and Genome Editing Techniques for Food and Nutritional Security

“…This approach is essential for characterizing the plant’s physiological response to pathogens and elucidating the signaling events during pathosystem development. For example, NGS revealed a candidate gene network underlying the resistance of barley to Blumeria graminis [ 113 ], unraveled the broad spectrum of potential molecular events associated with plant susceptible response to Pectobacterium atrosepticum -caused infection [ 114 ], elucidated gene expression patterns that are characteristic of compatible and incompatible interactions between potato plants and Phytophthora infestans [ 115 ], and pointed to the effects of viral infection on phytohormone metabolism [ 116 ], etc. In addition, NGS is also used to study non-infectious diseases and plant adaptation to unfavorable abiotic conditions.…”