Comparative Transcript Levels of Sugar Metabolism Genes Between the Canary Melon and a Vietnamese Non-Sweet Melon Cultivar

Abstract

Two different melon cultivars, Canary and Vietnamese non-sweet melons, are used to compare the fruit's sweetness levels. The results indicate that the Canary melon is much sweeter than the non-sweet melon. The transcript levels of the sugar metabolism genes, including <em>Cucumis melo ACID INVERTASE 2</em> (<em>CmAIN2</em>) and <em>SUCROSE SYNTHASE 1</em> (<em>CmSUS1</em>), are examined in two fruit tissues. PCR using cDNA and the electrophoresis assays indicate that the <em>CmAIN2</em> and <em>CmSUS1 </em>primer sets are specific, and only one band of PCR product is obtained from all tested samples. The quantitative reverse transcription PCR (RT-qPCR) assay is applied to compare the transcript levels of the <em>CmAIN2</em> and <em>CmSUS1 </em>genes in fruit tissues of the Canary and the Vietnamese non-sweet melons. Consistent with the sweetness levels, the <em>CmAIN2 </em>and <em>CmSUS1 </em>transcript levels are higher in the Canary melon than those in the non-sweet melon. These results imply that the local sugar metabolism in the fruits may also play an essential role in determining fruit sweetness. In addition, practically, the transcript levels of the <em>CmAIN2 </em>and <em>CmSUS1 </em>genes can be accessed and used to predict the sweetness of melon fruits early.