Comparative toxicity of the plasticizer dibutyl phthalate to two freshwater algae

Gu, Shurui; Zheng, Hao; Xu, Qingqing; Sun, Cuizhu; Shi, Mei; Wang, Zhenyu; Li, Fengmin

doi:10.1016/j.aquatox.2017.08.007

Cited by 92 publications

(27 citation statements)

References 36 publications

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“…The median effective concentration (EC 50 ) of DBP for C. vulgaris was 4.9 mg L −1 . The latest study indicated the 96-hour median effective concentration values (96h-EC 50 ) of DBP on two typical freshwater algae (Scenedesmus obliquus and Chlorella pyrenoidosa) were 15.3 mg L −1 and 3.14 mg L −1 , respectively [55]. Figure 3 shows the percentage of chlorophyll a of C. vulgaris compared with the blank after DBP phytoremediation.…”

Section: Algal Biotoxicity Of Dbpmentioning

confidence: 98%

Characterization of Di-n-Butyl Phthalate Phytoremediation by Garden Lettuce (Lactuca sativa L. var. longifolia) through Kinetics and Proteome Analysis

2019

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Di-n-dutyl phthalate (DBP), an endocrine disruptor, is one of the most widely used phthalate esters (PAEs) in the world. It can be accumulated in seafood or agricultural products and represents a substantial risk to human health via the food chain. Thus, finding a plant which can remediate DBP but have no effects on growth is the main topic of the development of DBP phytoremediation. This study used garden lettuce (Lactuca sativa L. var. longifolia), which has a significant DBP absorption capability, as a test plant to measure phytoremediation kinetics and proteome changes after being exposed to DBP. The results show that DBP accumulated in different parts of the garden lettuce but the physiological status and morphology showed no significant changes following DBP phytoremediation. The optimal condition for the DBP phytoremediation of garden lettuce is one critical micelle concentration (CMC) of non-ionic surfactant Tween 80 and the half-life (t1/2, days), which calculated by first-order kinetics, was 2.686 days for 5 mg L−1 of DBP. This result indicated that the addition of 1 CMC of Tween 80 could enhance the efficiency of DBP phytoremediation. In addition, the results of biotoxicity showed that the median effective concentration (EC50) of DBP for Chlorella vulgaris is 4.9 mg L−1. In this case, the overall toxicity markedly decreased following phytoremediation. In the end, the result of proteome analysis showed six protein spots, revealing significant alterations. According to the information of these proteomes, DBP potentially causes osmotic and oxidative stress in garden lettuce. In addition, since DBP had no significant effects on the morphology and physiological status of garden lettuce, garden lettuce can be recommended for use in the plant anti-DBP toxicity test, and also as the candidate plant for DBP phytoremediation. We hope these findings could provide valuable information for DBP-contaminated water treatment in ecological engineering applications or constructed wetlands.

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Section: Algal Biotoxicity Of Dbpmentioning

confidence: 98%

Characterization of Di-n-Butyl Phthalate Phytoremediation by Garden Lettuce (Lactuca sativa L. var. longifolia) through Kinetics and Proteome Analysis

2019

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“…Our previous report demonstrated that the acute biotoxicity of DBP to C. vulgaris after 24 h of exposure could decrease the chlorophyll a content [ 41 ]. Another study indicated that DBP caused growth inhibition and the 96h-EC 50 of DBP on two typical freshwater algae ( Scenedesmus obliquus and Chlorella pyrenoidosa ) was 15.3 and 3.14 mg L −1 , respectively [ 42 ] . The other research study demonstrated that DBP impacted growth rate and chlorophyll concentration in three algae [ 32 ] .…”

Section: Resultsmentioning

confidence: 99%

Impacts of Endocrine Disruptor di-n-Butyl Phthalate Ester on Microalga Chlorella vulgaris Verified by Approaches of Proteomics and Gene Ontology

et al. 2020

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Di-n-butyl phthalate (DBP) is an extensively used plasticizer. Most investigations on DBP have been concentrated on its environmental distribution and toxicity to humans. However, information on the effects of plasticizers on algal species is scarce. This study verified the impacts of endocrine disruptor di-n-butyl phthalate ester on microalga Chlorella vulgaris by approaches of proteomics and gene ontology. The algal acute biotoxicity results showed that the 24h-EC50 of DBP for C. vulgaris was 4.95 mg L−1, which caused a decrease in the chlorophyll a content and an increase in the DBP concentration of C. vulgaris. Proteomic analysis led to the identification of 1257 C. vulgaris proteins. Sixty-one more proteins showed increased expression, compared to proteins with decreased expression. This result illustrates that exposure to DBP generally enhances protein expression in C. vulgaris. GO annotation showed that both acetolactate synthase (ALS) and GDP-L-fucose synthase 2 (GER2) decreased more than 1.5-fold after exposure to DBP. These effects could inhibit both the valine biosynthetic process and the nucleotide-sugar metabolic process in C. vulgaris. The results of this study demonstrate that DBP could inhibit growth and cause significant changes to the biosynthesis-relevant proteins in C. vulgaris.

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“…Saarma et al (2003) reported the toxic effect of DEP in retarding the growth of radish (Raphanus sativus). Gu et al (2017) reported inhibition of algal growth under DBP stress.…”

Section: Discussionmentioning

confidence: 99%

Elucidating physiological and biochemical alterations in giant duckweed (Spirodela polyrhizaL. Schleiden) under diethyl phthalate stress: insights into antioxidant defence system

Sharma

Kaur

2020

PeerJ

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Background The emollient properties of phthalates have led to their extensive production and intense use in plastic products. Owing to their weak covalent bonding with the plastic polymers, phthalates enter into the environment during their manufacturing, processing, disposal, consequently found their way directly into water sources, soil, and sediments. Methods The present study envisaged the toxic effects of diethyl phthalate (DEP) on physiological and biochemical attributes of Spirodela polyrhiza, when exposed to various concentrations of DEP (0, 10, 20, 40, 80, 100, 200, and 400 ppm) for short term exposure period of seven days. Results Plants of S. polyrhiza accumulated significant amount of DEP (112 mg kg−1 fw) when exposed to various concentrations of DEP for seven days. Results depicted that DEP toxicity significantly (p ≤ 0.05) affected growth parameters and pigments in treated S. polyrhiza as compared to control. Further, high doses of DEP (400 ppm) caused significant decrement in carbohydrate (86%), protein (76%) and elevation in MDA content (42%). Meanwhile, DEP altered the activities of antioxidant enzymes (SOD, CAT, APX, GPX and GR) along with the induction of enhanced levels of proline, electrolyte leakage and phenolic content. Scanning electron microscopic and confocal studies also confirmed oxidative stress in plants under DEP stress. Conclusions Present findings will help understand the accumulation, tolerance, and detoxification mechanisms of DEP by S. polyrhiza to counteract the effects of reactive oxygen species (ROS), along with the evaluation of environmental threat for aquatic plants in aquatic ecosystems.

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Comparative toxicity of the plasticizer dibutyl phthalate to two freshwater algae

Cited by 92 publications

References 36 publications

Characterization of Di-n-Butyl Phthalate Phytoremediation by Garden Lettuce (Lactuca sativa L. var. longifolia) through Kinetics and Proteome Analysis

Characterization of Di-n-Butyl Phthalate Phytoremediation by Garden Lettuce (Lactuca sativa L. var. longifolia) through Kinetics and Proteome Analysis

Impacts of Endocrine Disruptor di-n-Butyl Phthalate Ester on Microalga Chlorella vulgaris Verified by Approaches of Proteomics and Gene Ontology

Elucidating physiological and biochemical alterations in giant duckweed (Spirodela polyrhizaL. Schleiden) under diethyl phthalate stress: insights into antioxidant defence system

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