Comparative toxicity of oleic acid and linoleic acid on Jurkat cells

Cury-Boaventura, Maria Fernanda; Pompéia, Celine; Curi, Rui

doi:10.1016/j.clnu.2003.12.004

Cited by 147 publications

(107 citation statements)

References 40 publications

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“…The cytotoxic activity of the -lactoglobulin and paralbumin complexes even exceeded that of HAMLET, indicating that the proteinaceous component of the complexes is of less importance than the OA component in relation to the cytotoxic activity [40]. The potent toxicity of free oleic acid against Jurkat and HL-60 tumor cells has been shown, supporting the proposal that the fatty acid could be the cytotoxic component of the HAMLET complex [54,55]. However, in contrast with this proposal, -LA has previously been found to be cytotoxic in the absence of oleic acid [56][57][58][59][60].…”

Section: Characteristics Of Hamletsupporting

confidence: 54%

The Alpha-Lactalbumin/Oleic Acid Complex and Its Cytotoxic Activity

Jøhnke¹,

Petersen²

2012

Milk Protein

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Section: Characteristics Of Hamletsupporting

confidence: 54%

The Alpha-Lactalbumin/Oleic Acid Complex and Its Cytotoxic Activity

Jøhnke¹,

Petersen²

2012

Milk Protein

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“…Early apoptosis is characterized by the translocation of PS from the inner layer of the plasma membrane to the outer surface (Cury-Boaventura et al, 2004). Apoptotic cells are reflected by quantification of Annexin V-FITC binding to externalized PS.…”

Section: E Hirta Extract Induced Apoptosis In Mcf-7 Cellsmentioning

confidence: 99%

Evaluation of the cytotoxicity, cell-cycle arrest, and apoptotic induction byEuphorbia hirtain MCF-7 breast cancer cells

Kwan

Saito

Demirtaş

et al. 2015

Pharmaceutical Biology

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Context: Euphorbia hirta L. (Euphorbiaceae) has been used as a folk remedy in Southeast Asia for the treatment of various ailments. Objective: The current study evaluates the cytotoxicity, cell-cycle arrest, and apoptotic induction by E. hirta in MCF-7 breast cancer cells. Materials and methods: Cytotoxic activity of methanol extract of whole part of E. hirta was determined by the MTT assay at various concentrations ranging from 1.96 to 250.00 mg/mL in MCF-7 cells. Cell morphology was assessed by light and fluorescence microscopy. Apoptosis and cell-cycle distribution were determined by annexin V staining and flow cytometry. DNA fragmentation, caspase activity, and reactive oxygen species (ROS) assays were performed using the commercially available kits. To identify the cytotoxic fraction, E. hirta extract was subjected to bioassay-guided fractionation. Results: Euphorbia hirta exhibited significant inhibition of the survival of MCF-7 cells and the half inhibitory concentration (IC 50 ) values was 25.26 mg/mL at 24 h. Microscopic studies showed that E. hirta-treated cells exhibited marked morphological features characteristic of apoptosis. Euphorbia hirta extract also had an ignorable influence on the LDH leakage and generating intracellular ROS. The flow cytometry study confirmed that E. hirta extract induced apoptosis in MCF-7 cells. Euphorbia hirta also resulted in DNA fragmentation in MCF-7 cells. Moreover, E. hirta treatment resulted in the accumulation of cells at the S and G 2 /M phases as well as apoptosis. The caspase activity study revealed that E. hirta extract induced apoptosis through the caspase-3-independent pathway by the activation of caspase-2, 6, 8, and 9. Euphorbia hirta hexane fraction, namely HFsub4 fraction, demonstrated highest activity among all the fractions tested with an IC 50 value of 10.01 mg/mL at 24 h. Discussion and conclusion: This study revealed that E. hirta induced apoptotic cell death and suggests that E. hirta could be used as an apoptosis-inducing anticancer agent for breast cancer treatment with further detailed studies.

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“…Consequently, investigators have focused on anticancer constituents in supercritical CO 2 extracts. Accumulating evidence suggests that these extracts have several possible anticancer agents, including caryophyllene oxide (Park et al, 2011), β-caryophyllene (Jun et al, 2011), limonene (Crowell et al, 1994), nobiletin (Yoshimizu et al, 2004), tangeretin (Chaumontet et al, 1997), squalene (Reddy et al, 2009), hexadecanoic acid (Ji et al, 2005, (Z)-9-octadecenoic acid (Cury-Boaventura et al, 2004), alpha-terpineol (Hassan et al, 2010), and γ-sitosterol (Khan and Mlungwana, 1999). Additionally, phytosterols were also detected in comparatively good amounts (3.34 − 8.48 %).…”

Section: Gc-ms Analysis Chromatographic Analysis Was Carried Out Usinmentioning

confidence: 99%

“…A one-way analysis of variance (ANOVA) and LSD were conducted using the Statistical Package for the Social Sciences software (SPSS 17.0 for Windows, 2008, SPSS Inc., Chicago, IL, USA). A p < 0.05 Unsaturated ω 3 and ω 6 series fatty acids and several saturated fatty acids play an important role modulating human metabolism (Cury-Boaventura et al, 2004, Lee et al, 2001, Ji et al, 2005. Thus, several studies concerning lipid compounds of citrus have been carried out (Saidani et al, 2004, Pellegrini et al, 2003, Tounsi et al, 2011.…”

Section: Gc-ms Analysis Chromatographic Analysis Was Carried Out Usinmentioning

confidence: 99%

Chemical Composition and Antiproliferative Activity of Supercritical CO2 Extracts from Citrus Fruits

Gyawali

Moon

Jeon

et al. 2012

FSTR

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Supercritical CO 2 extract from fruits of Citrus hassaku Hort ex Tanaka, Citrus iyo Hort. ex Tanaka, and Citrus grandis Osbeck, locally known as phalsak, yeagam, and dangyuja, respectively, in Korea, were analyzed by gas chromatography-mass spectrometry (GC-MS). In total, 70, 79, and 76 compounds were identified in fruits of phalsak, yeagam, and dangyuja respectively. (Z)-9-Octadecenoic acid was the major compound in phalsak, whereas limonene prevailed among the others. α-Terpineol, (E,E)-2,4-decadienal, hexadecanoic acid, pentacosane, stigmasterol, and γ-sitosterol were prominent in all species. Antiproliferative activity of the fruit extract was revealed through the colorimetric MTT (tetrazolium) assay against human glioblastoma U373MG, SNU-16 gastric cancer cells, HeLa cervix adenocarcinoma cells and AGS gastric adenocarcinoma cells. Although all tested fruit extracts exhibited antiproliferative effects toward cancer cells, different cell lines varied in their sensitivity to the same plant fruit extract.

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Comparative toxicity of oleic acid and linoleic acid on Jurkat cells

Cited by 147 publications

References 40 publications

The Alpha-Lactalbumin/Oleic Acid Complex and Its Cytotoxic Activity

The Alpha-Lactalbumin/Oleic Acid Complex and Its Cytotoxic Activity

Evaluation of the cytotoxicity, cell-cycle arrest, and apoptotic induction byEuphorbia hirtain MCF-7 breast cancer cells

Chemical Composition and Antiproliferative Activity of Supercritical CO2 Extracts from Citrus Fruits

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