Comparative testing of HPV L1 protein monoclonal antibody panel for the detection of HPV in cervical exfoliated cells

Wang, Le; Hu, Yongfeng; Changyi, Xiao; Wang, Fei; Liu, Yu-Fei; Tang, Li-Hua; Xiao, Rong-Shuang

doi:10.1016/j.jviromet.2018.03.012

Cited by 7 publications

(2 citation statements)

References 28 publications

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“…For instance, a mouse mAb panel against a C-terminal conserved sequence polypeptides of human papillomavirus (HPV) L1, a major capsid protein, by using the IHC method used for the detection of HPV in exfoliated cervical cells. The detection rate was comparable to that obtained using the commercial HPV genotyping kit currently used in clinical practice (36). Another study also showed that HPV L1 capsid detection in cervical exfoliated cells is useful for screening cervical lesions in high-risk HPV-positive women, and it may be a promising triage for high-risk women with HPV-positive atypical squamous cell of undetermined significance (ASCUS) (37).…”

Section: Detection Of Hpv Protein Antigens In Tumor Tissues and Cellsmentioning

confidence: 62%

Immunodiagnosis and Immunotherapeutics Based on Human Papillomavirus for HPV-Induced Cancers

Dong

et al. 2021

Front. Immunol.

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Infection with human papillomavirus (HPV) is one of the main causes of malignant neoplasms, especially cervical, anogenital, and oropharyngeal cancers. Although we have developed preventive vaccines that can protect from HPV infection, there are still many new cases of HPV-related cancers worldwide. Early diagnosis and therapy are therefore important for the treatment of these diseases. As HPVs are the major contributors to these cancers, it is reasonable to develop reagents, kits, or devices to detect and eliminate HPVs for early diagnosis and therapeutics. Immunological methods are precise strategies that are promising for the accurate detection and blockade of HPVs. During the last decades, the mechanism of how HPVs induce neoplasms has been extensively elucidated, and several oncogenic HPV early proteins, including E5, E6, and E7, have been shown to be positively related to the oncogenesis and malignancy of HPV-induced cancers. These oncoproteins are promising biomarkers for diagnosis and as targets for the therapeutics of HPV-related cancers. Importantly, many specific monoclonal antibodies (mAbs), or newly designed antibody mimics, as well as new immunological kits, devices, and reagents have been developed for both the immunodiagnosis and immunotherapeutics of HPV-induced cancers. In the current review, we summarize the research progress in the immunodiagnosis and immunotherapeutics based on HPV for HPV-induced cancers. In particular, we depict the most promising serological methods for the detection of HPV infection and several therapeutical immunotherapeutics based on HPV, using immunological tools, including native mAbs, radio-labelled mAbs, affitoxins (affibody-linked toxins), intracellular single-chain antibodies (scFvs), nanobodies, therapeutical vaccines, and T-cell-based therapies. Our review aims to provide new clues for researchers to develop novel strategies and methods for the diagnosis and treatment of HPV-induced tumors.

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Section: Detection Of Hpv Protein Antigens In Tumor Tissues and Cellsmentioning

confidence: 62%

Immunodiagnosis and Immunotherapeutics Based on Human Papillomavirus for HPV-Induced Cancers

Dong

et al. 2021

Front. Immunol.

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“…The antigen-capture enzyme linked immunosorbent assay (AC-ELISA) and colloidal gold immunochromatographic test (ICT) strip approaches have been established using MAbs [28,29]. Many viruses can be detected using detection methods involving MAbs, such as rabies virus, human papillomavirus, and human enterovirus [30][31][32]. In the eld of in uenza virus detection, AC-ELISA can be developed to evaluate vaccine e cacy and to diagnose infections, and ICT strips have also been used to detect the AIVs, showing good speci city and sensitivity for samples from patients and poultry infected with AIVs [33][34][35].…”

Section: Introductionmentioning

confidence: 99%

Antigen-Capture ELISA And Immunochromatographic Test Strip To Detect The H9N2 Subtype Avian Influenza Virus Rapidly Based On Monoclonal Antibodies

Xiao

Yang

Liu

et al. 2021

Preprint

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Background: The H9N2 subtype of avian influenza virus (AIV) has become the most widespread subtype of AIV among birds in Asia, which threatens the poultry industry and human health. Therefore, it is important to establish methods for the rapid diagnosis and continuous surveillance of H9N2 subtype AIV.Methods: In this study, an antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) and a colloidal gold immunochromatographic test (ICT) strip using monoclonal antibodies (MAbs) 3G4 and 2G7 were established to detect H9N2 subtype AIV. Results: The AC-ELISA method and ICT strip can detect H9N2 subtype AIV quickly, and do not cross-react with other subtype AIVs or other viruses. The detection limit of AC-ELISA was a hemagglutinin (HA) titer of 4 for H9N2 subtype AIV per 100 μl sample, and the limit of detection of the HA protein of AIV H9N2 was 31.5 ng/ml. The ICT strip detection limit was an HA titer of 4 for H9N2 subtype AIV per 100 μl sample. Moreover, both detection methods exhibited good reproducibility and repeatability, with coefficients of variation < 5%. For detection in 200 actual poultry samples, the sensitivities and specificities of AC-ELISA were determined as 93.2% and 98.1%, respectively. The sensitivities and specificities of the ICT strips were determined as 90.9% and 97.4%, respectively. Conclusions: The developed AC-ELISA and ICT strips displayed high specificity, sensitivity, and stability, making them suitable for rapid diagnosis and field investigation of H9N2 subtype AIV.

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An update on antiviral antibody-based biopharmaceuticals

Ahangarzadeh

Payandeh

Arezumand

et al. 2020

International Immunopharmacology

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Due to the vastness of the science virology, it is no longer an offshoot solely of the microbiology. Viruses have become as the causative agents of major epidemics throughout history. Many therapeutic strategies have been used for these microorganisms, and in this way the recognizing of potential targets of viruses is of particular importance for success. For decades, antibodies and antibody fragments have occupied a significant body of the treatment approaches against infectious diseases. Because of their high affinity, they can be designed and engineered against a variety of purposes, mainly since antibody fragments such as scFv, nanobody, diabody, and bispecific antibody have emerged owing to their small size and interesting properties. In this review, we have discussed the antibody discovery and molecular and biological design of antibody fragments as inspiring therapeutic and diagnostic agents against viral targets.

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Comparative testing of HPV L1 protein monoclonal antibody panel for the detection of HPV in cervical exfoliated cells

Cited by 7 publications

References 28 publications

Immunodiagnosis and Immunotherapeutics Based on Human Papillomavirus for HPV-Induced Cancers

Immunodiagnosis and Immunotherapeutics Based on Human Papillomavirus for HPV-Induced Cancers

Antigen-Capture ELISA And Immunochromatographic Test Strip To Detect The H9N2 Subtype Avian Influenza Virus Rapidly Based On Monoclonal Antibodies

An update on antiviral antibody-based biopharmaceuticals

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