Comparative suppression of NLRP3 inflammasome activation with LPS-induced inflammation by blueberry extracts (Vaccinium spp.)

Wang, Huailing; Guo, Xinbo; Liu, Jie; Li, Tong; Fu, Xiong; Liu, Rui Hai

doi:10.1039/c7ra02562a

Cited by 18 publications

(19 citation statements)

References 46 publications

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“…Our data evidenced the remarkable activity of BB in preventing the increased aforementioned mRNA levels and the oligomerization of the proteins NLRP1 and ASC, upon O 3 exposure. These data confirmed the study by Wang et al which demonstrated reduced gene expression levels of NLRP, Caspase 1, ASC, and proinflammatory cytokines IL-1β, TNF-α, IL-6, and iNOS in macrophages pretreated with BB extracts and then challenged with lipopolysaccharide [91].…”

supporting

confidence: 91%

Blueberry Extracts as a Novel Approach to Prevent Ozone-Induced Cutaneous Inflammasome Activation

Pambianchi

Ferrara

Pecorelli

et al. 2020

Oxidative Medicine and Cellular Longevity

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The World Health Organization estimates that 7 million people die every year due to pollution exposure. Among the different pollutants to which living organism are exposed, ozone (O3) represents one of the most toxic, because its location which is the skin is one of the direct tissues exposed to the outdoor environment. Chronic exposure to outdoor stressors can alter cutaneous redox state resulting in the activation of inflammatory pathways. Recently, a new player in the inflammation mechanism was discovered: the multiprotein complex NLRP1 inflammasome, which has been shown to be also expressed in the skin. The topical application of natural compounds has been studied for the last 40 years as a possible approach to prevent and eventually cure skin conditions. Recently, the possibility to use blueberry (BB) extract to prevent pollution-induced skin toxicity has been of great interest in the cosmeceutical industry. In the present study, we analyzed the cutaneous protective effect of BB extract in several skin models (2D, 3D, and human skin explants). Specifically, we observed that in the different skin models used, BB extracts were able to enhance keratinocyte wound closure and normalize proliferation and migration responses previously altered by O3. In addition, pretreatment with BB extracts was able to prevent ozone-induced ROS production and inflammasome activation measured as NRLP1-ASC scaffold formation and also prevent the transcripts of key inflammasome players such as CASP1 and IL-18, suggesting that this approach as a possible new technology to prevent cutaneous pollution damage. Our data support the hypothesis that BB extracts can effectively reduce skin inflammation and be a possible new technology against cutaneous pollution-induced damage.

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supporting

confidence: 91%

Blueberry Extracts as a Novel Approach to Prevent Ozone-Induced Cutaneous Inflammasome Activation

Pambianchi

Ferrara

Pecorelli

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…The anti-inflammatory mechanism of blueberry in LPSstimulated RAW 264.7 macrophages was hypothesized to be due to suppression of oxidative stress and reduced expression of proinflammatory cytokines (Watson & Preedy, 2014). In addition to the above mentioned pro-inflammatory markers, blueberry extracts significantly inhibited the gene expression of TNF-a and IL-12 in mononuclear macrophage model, and MMP-2, MMP-9 in human corneal epithelial cells (Cheng et al, 2014;Wang et al, 2017a).…”

Section: Effect Of Blueberry Extracts On Inflammatory Markers-exposurementioning

confidence: 95%

Simultaneous LC-MS quantification of anthocyanins and non-anthocyanin phenolics from blueberries with widely divergent profiles and biological activities

et al. 2019

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A sensitive and straightforward LC-IT-TOF-MS method was validated for the profiling and simultaneous quantification of anthocyanins, flavan-3-ols, flavonols, phenolic acids, and resveratrol in blueberry genotypes with fruit color ranging from deep purple (Vaccinium angustifolium) to various shades of pink (crosses of V. corymbosum, V. darrowii, and V. ashei). Standard calibration curves were linear for all analytes with correlation coefficients >0.99. The relative standard deviation for intra-and inter-day precision was lower than 10%. The method allowed an easy and selective identification and quantification of phenolics in blueberries with divergent profiles. The in vitro antioxidant assay results were strongly correlated with total phenolics and total anthocyanin content. Lowbush blueberry extracts (50 μg/mL) reduced ROS and NO production, and inhibited the transcription of the proinflammatory cytokines IL-6β, COX2, iNOS, and IL-6 in the in vitro assays at much lower concentrations than pink fruited berries (250 μg/mL).

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“…Within a plant extract, there can be hundreds of molecules, some of which are active individually or act synergistically against a given disease. In most of the cases, major compounds are responsible of the activity of the extract though it is not always the case [ 35 , 36 ]. In this study, an important question was to determine whether the inhibition of NLRP3 inflammasome resulted from a single compound or from a combined effect of different compounds.…”

Section: Discussionmentioning

confidence: 99%

“…Such reduction suggested the presence of an inhibitor of caspase-1 in the plant extract. Indeed, caffeic acid, purified from H. noldeae in this work, was reported to interact with and inhibits Caspase-1 and ASC activation [ 36 ]. The plateau could be explained by the fact that a saturated quantity of caffeic acid is already in the lowest sample concentration used (5 µg/mL).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have reported in vivo anti-inflammatory properties of caffeic acid and isoquercetin as well as several corresponding derivatives [ 36 , 38 , 39 ]. In this study, as suggested by our results, several polyphenolic compounds detected in the plant extracts of H. noldeae likely contribute to the inhibitory property of the plant on the inflammasome expression.…”

Section: Discussionmentioning

confidence: 99%

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The Inhibition of NLRP3 Inflammasome and IL-6 Production by Hibiscus noldeae Baker f. Derived Constituents Provides a Link to Its Anti-Inflammatory Therapeutic Potentials

et al. 2020

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The activation of NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome and/or its components is associated with the physio-pathogenesis of many respiratory diseases including asthma, COPD (chronic obstructive pulmonary disease), SARS Cov-2 (severe acute respiratory syndrome coronavirus 2), and in several autoimmune diseases. Hibiscus noldeae Baker f. has been widely reported to be traditionally used in the treatment of different ailments, some of which are of inflammatory background such as asthma, wounds, headache, etc. However, the claims have not been supported by evidence at the molecular and functional levels. Here, we report on the bio-guided fractionation of H. noldeae and assessment of the inhibitory properties of some fractions and purified compounds on NLRP3 inflammasome and Interleukin 6 (IL-6). The activation of the NLRP3 inflammasome was determined by detecting the activity of caspase-1 and the production of Interleukin 1β (IL-1β) in Lipopolysaccharide (LPS) and ATP-stimulated Tamm-Horsfall Protein 1 (THP-1) macrophages, while the production of IL-6 was studied in LPS-stimulated RAW264.7 mouse macrophages. It was observed that hexane and ethyl acetate fractions of the crude extract of the aerial parts of H. noldeae, as well as caffeic acid, isoquercetin, and ER2.4 and ER2.7 fractions revealed significant inhibitory effects on Caspase-1 activities, and on IL-1β and IL-6 production. The ER2.4 and ER2.7 fractions downregulated the production of IL-1β and IL-6, in a similar range as the caspase-1 inhibitor AC-YVAD-CHO and the drug Dexamethasone, both used as controls, respectively. Overall, our work does provide the very first scientific based evidence for Hibiscus noldeae anti-inflammatory effects and widespread use by traditional healers in Rwanda for a variety of ailments.

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Comparative suppression of NLRP3 inflammasome activation with LPS-induced inflammation by blueberry extracts (Vaccinium spp.)

Abstract: The aim of this study was to evaluate the anti-inflammation effects of blueberry extracts through NLRP3 inflammasome.

Cited by 18 publications

References 46 publications

Blueberry Extracts as a Novel Approach to Prevent Ozone-Induced Cutaneous Inflammasome Activation

Blueberry Extracts as a Novel Approach to Prevent Ozone-Induced Cutaneous Inflammasome Activation

Simultaneous LC-MS quantification of anthocyanins and non-anthocyanin phenolics from blueberries with widely divergent profiles and biological activities

The Inhibition of NLRP3 Inflammasome and IL-6 Production by Hibiscus noldeae Baker f. Derived Constituents Provides a Link to Its Anti-Inflammatory Therapeutic Potentials

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